RNF12 (RLIM) (NM_183353) Human Tagged ORF Clone

SKU RC203442

RLIM (Myc-DDK-tagged)-Human ring finger protein, LIM domain interacting (RLIM), transcript variant 2

Product Data

• Target Symbol: RNF12
• Synonyms: MRX61; NY-REN-43; RNF12; TOKAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203442 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCAGCACAGCGCAGAAGTCAGATGG; ACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTAAATAACCTGAGTGAAGAAGATTATAGGCTTAT; GAGAGATAACAATTTGCTAGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAA; ATTAAAGAAGGCCCACCACCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTGTCTA; ATGGTGACTCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGGCAAAG; AGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGATTCAGTTTAGAG; ATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAATGAGCCATCTGCAAGACGTTCTA; GTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTGGAAAACCCACGATCTGAATCAACATCTGCAAG; GCCATCTAGATCAGAACGAAATTCAACTGAAGCGTTAACAGAGGTCCCACCTACCAGAGGTCAGAGGAGG; GCAAGAAGCAGGAGCCCAGACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCACCTCTGCATC; CAATGAGTGAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACATCCTTTGGTAAA; TGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCTGGGCCTGAGTTG; CTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAAGGAGCAGGTTCTTCAGACACAG; CTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCTCCAACCATAGTCCTTGATCTTCAAGTAAGAAG; AGTTCGTCCTGGAGAATATCGGCAGAGAGATAGCATAGCCAGCAGAACTCGGTCTAGGTCTCAGACACCA; AACAACACTGTCACCTATGAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCACGTTCTGAGCGGGCAG; GTGTGAGAACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAATACTGGTTTAAGTGAGAC; TACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGTGAGTTAAGCTATTTTATG; TACAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAATATGGAAAGGGCAGAGTCACGGA; GTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGTTCCAGTTCGAGTTCCAGTTCCAGTTCGAGTTC; CAGTTCCAGTTCAAGTTCCAGTTCCAGTCCTAGTTCCAGTTCCGGTGGTGAAAGTTCAGAAACTAGCTCA; GATTTATTTGAAGGCAGTAATGAAGGAAGCTCATCATCAGGCTCATCAGGTGCCAGGCGAGAGGGTCGAC; ATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTTAGCCTGGCTCAGTTTTTCCTCTT; AAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAGATTGACAACTTGGCAATGAGAAGT; TTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACAGAATATACAGAAGGCAACAAACTTC; GTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGCATCGATCGCTGGTTATCTGAGAATTCTACCTG; TCCTATTTGTCGCAGAGCAGTCTTAGCTTCTGGTAACAGAGAAAGTGTTGTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203442 protein sequence; Red=Cloning site Green=Tags(s); ; MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQ; IKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLE; INVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRR; ARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPEL; LSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTP; NNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFM; YSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSS; DLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRS; FGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183353
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_183353.3
• RefSeq Size: 10652 bp
• RefSeq ORF: 1875 bp
• Locus ID: 51132
• UniProt ID: Q9NVW2
• Cytogenetics: Xq13.2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 68.5 kDa
• Summary: The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]