Hsp75 (TRAP1) (NM_016292) Human Tagged ORF Clone

CAT#: RC203439

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TRAP1 (Myc-DDK-tagged)-Human TNF receptor-associated protein 1 (TRAP1)



  "NM_016292" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRAP1
Synonyms HSP 75; HSP75; HSP90L; TRAP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203439 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGCGAGCTGCGGGCGCTGCTGCTGTGGGGCCGCCGCCTGCGGCCTTTGCTGCGGGCGCCGGCGC
TGGCGGCCGTGCCGGGAGGAAAACCAATTCTGTGTCCTCGGAGGACCACAGCCCAGTTGGGCCCCAGGCG
AAACCCAGCCTGGAGCTTGCAGGCAGGACGACTGTTCAGCACGCAGACCGCCGAGGACAAGGAGGAACCC
CTGCACTCGATTATCAGCAGCACAGAGAGCGTGCAGGGTTCCACTTCCAAACATGAGTTCCAGGCCGAGA
CAAAGAAGCTTTTGGACATTGTTGCCCGGTCCCTGTACTCAGAAAAAGAGGTGTTTATACGGGAGCTGAT
CTCCAATGCCAGCGATGCCTTGGAAAAACTGCGTCACAAACTGGTGTCTGACGGCCAAGCACTGCCAGAA
ATGGAGATTCACTTGCAGACCAATGCCGAGAAAGGCACCATCACCATCCAGGATACTGGTATCGGGATGA
CACAGGAAGAGCTGGTGTCCAACCTGGGGACGATTGCCAGATCGGGGTCAAAGGCCTTCCTGGATGCTCT
GCAGAACCAGGCTGAGGCCAGCAGCAAGATCATCGGCCAGTTTGGAGTGGGTTTCTACTCAGCTTTCATG
GTGGCTGACAGAGTGGAGGTCTATTCCCGCTCGGCAGCCCCGGGGAGCCTGGGTTACCAGTGGCTTTCAG
ATGGTTCTGGAGTGTTTGAAATCGCCGAAGCTTCGGGAGTTAGAACCGGGACAAAAATCATCATCCACCT
GAAATCCGACTGCAAGGAGTTTTCCAGCGAGGCCCGGGTGCGAGATGTGGTAACGAAGTACAGCAACTTC
GTCAGCTTCCCCTTGTACTTGAATGGAAGGCGGATGAACACCTTGCAGGCCATCTGGATGATGGACCCCA
AGGATGTCCGTGAGTGGCAACATGAGGAGTTCTACCGCTACGTCGCGCAGGCTCACGACAAGCCCCGCTA
CACCCTGCACTATAAGACGGACGCACCGCTCAACATCCGCAGCATCTTCTACGTGCCCGACATGAAACCG
TCCATGTTTGATGTGAGCCGGGAGCTGGGCTCCAGCGTTGCACTGTACAGCCGCAAAGTCCTCATCCAGA
CCAAGGCCACGGACATCCTGCCCAAGTGGCTGCGCTTCATCCGAGGTGTGGTGGACAGTGAGGACATTCC
CCTGAACCTCAGCCGGGAGCTGCTGCAGGAGAGCGCACTCATCAGGAAACTCCGGGACGTTTTACAGCAG
AGGCTGATCAAATTCTTCATTGACCAGAGTAAAAAAGATGCTGAGAAGTATGCAAAGTTTTTTGAAGATT
ACGGCCTGTTCATGCGGGAGGGCATTGTGACCGCCACCGAGCAGGAGGTCAAGGAGGACATAGCAAAGCT
GCTGCGCTACGAGTCCTCGGCGCTGCCCTCCGGGCAGCTAACCAGCCTCTCAGAATACGCCAGCCGCATG
CGGGCCGGCACCCGCAACATCTACTACCTGTGCGCCCCCAACCGTCACCTGGCAGAGCACTCACCCTACT
ATGAGGCCATGAAGAAGAAAGACACAGAGGTTCTCTTCTGCTTTGAGCAGTTTGATGAGCTCACCCTGCT
GCACCTTCGTGAGTTTGACAAGAAGAAGCTGATCTCTGTGGAGACGGACATAGTCGTGGATCACTACAAG
GAGGAGAAGTTTGAGGACAGGTCCCCAGCCGCCGAGTGCCTATCAGAGAAGGAGACGGAGGAGCTCATGG
CCTGGATGAGAAATGTGCTGGGGTCGCGTGTCACCAACGTGAAGGTGACCCTCCGACTGGACACCCACCC
TGCCATGGTCACCGTGCTGGAGATGGGGGCTGCCCGCCACTTCCTGCGCATGCAGCAGCTGGCCAAGACC
CAGGAGGAGCGCGCACAGCTCCTGCAGCCCACGCTGGAGATCAACCCCAGGCACGCGCTCATCAAGAAGC
TGAATCAGCTGCGCGCAAGCGAGCCTGGCCTGGCTCAGCTGCTGGTGGATCAGATATACGAGAACGCCAT
GATTGCTGCTGGACTTGTTGACGACCCTAGGGCCATGGTGGGCCGCTTGAATGAGCTGCTTGTCAAGGCC
CTGGAGCGACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203439 protein sequence
Red=Cloning site Green=Tags(s)

MARELRALLLWGRRLRPLLRAPALAAVPGGKPILCPRRTTAQLGPRRNPAWSLQAGRLFSTQTAEDKEEP
LHSIISSTESVQGSTSKHEFQAETKKLLDIVARSLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPE
MEIHLQTNAEKGTITIQDTGIGMTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFM
VADRVEVYSRSAAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNF
VSFPLYLNGRRMNTLQAIWMMDPKDVREWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNIRSIFYVPDMKP
SMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPLNLSRELLQESALIRKLRDVLQQ
RLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATEQEVKEDIAKLLRYESSALPSGQLTSLSEYASRM
RAGTRNIYYLCAPNRHLAEHSPYYEAMKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYK
EEKFEDRSPAAECLSEKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKT
QEERAQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRLNELLVKA
LERH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016292
ORF Size 2112 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016292.3
RefSeq Size 2310 bp
RefSeq ORF 2115 bp
Locus ID 10131
UniProt ID Q12931
Cytogenetics 16p13.3
Domains HSP90, HATPase_c
Protein Families Druggable Genome
MW 80.1 kDa
Gene Summary This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

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