ZNF16 (NM_006958) Human Tagged ORF Clone

CAT#: RC203436

ZNF16 (Myc-DDK-tagged)-Human zinc finger protein 16 (ZNF16), transcript variant 1

Product Images

Circular map for RC203436

• 

  Western blot validation of overexpression lysate (Cat# LY422273) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221669 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF16
• Synonyms: HZF1; KOX9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGCCTCAGAACTCGCCGTGAGGAGGCAGAGATGGAGCTCTCAGTTCCAGGACCATCCCCCTGGA
  CCCCTGCAGCCCAGGCCCGTGTGAGAGATGCTCCTGCTGTGACCCACCCTGGATCTGCAGCCTGTGGTAC
  CCCCTGCTGTAGTGATACTGAGCTGGAAGCCATCTGCCCTCACTATCAGCAGCCAGATTGTGACACCAGG
  ACTGAAGACAAGGAGTTTCTTCACAAGGAAGACATTCATGAAGATTTGGAATCACAGGCAGAAATATCAG
  AAAACTATGCTGGTGATGTTTCCCAGGTACCCGAGCTTGGAGATCTGTGTGATGATGTATCAGAAAGAGA
  CTGGGGAGTCCCCGAAGGCAGGAGGCTGCCACAGTCCCTCTCCCAGGAGGGGGACTTCACACCAGCTGCC
  ATGGGGCTCCTTAGGGGCCCCTTAGGGGAGAAAGATCTGGACTGTAATGGTTTTGACAGTCGCTTCAGTC
  TGAGCCCAAACCTGATGGCATGTCAGGAAATCCCTACAGAAGAGAGGCCACATCCATATGACATGGGTGG
  CCAGAGTTTCCAGCACAGTGTGGACCTAACTGGTCATGAGGGGGTTCCCACAGCTGAAAGTCCACTCATA
  TGTAATGAGTGTGGGAAAACCTTCCAAGGAAATCCTGACCTTATTCAGCGTCAAATAGTCCACACTGGGG
  AGGCTTCCTTTATGTGTGATGATTGTGGGAAAACCTTCAGCCAGAACTCAGTTCTTAAAAACCGTCATCG
  ATCTCATATGAGTGAGAAAGCTTACCAGTGCAGCGAATGTGGGAAAGCCTTCCGAGGGCACTCAGACTTT
  TCTAGGCATCAGAGTCACCACAGCAGTGAGAGGCCTTATATGTGTAATGAATGTGGAAAAGCCTTCAGCC
  AGAACTCGAGCCTTAAAAAGCACCAAAAGTCTCACATGAGTGAGAAGCCCTATGAATGCAATGAATGTGG
  GAAGGCTTTTAGGCGGAGCTCAAACCTCATCCAACATCAAAGAATCCATTCTGGGGAGAAACCGTATGTG
  TGCAGTGAGTGTGGGAAGGCCTTCAGGCGAAGCTCAAACCTCATCAAACACCACAGGACTCACACAGGAG
  AGAAGCCTTTTGAGTGTGGCGAGTGTGGGAAAGCCTTCAGCCAGAGTGCACACCTGAGGAAGCACCAGAG
  GGTCCACACTGGAGAGAAGCCTTATGAGTGTAATGATTGTGGCAAGCCCTTCAGTCGGGTCTCCAACCTC
  ATTAAGCACCACAGGGTTCACACTGGAGAGAAGCCCTATAAGTGCAGTGACTGTGGGAAAGCATTTAGTC
  AGAGCTCCAGCCTTATTCAGCATCGGAGAATTCACACTGGAGAAAAGCCTCACGTGTGTAATGTATGTGG
  AAAAGCCTTTAGTTATAGCTCAGTGCTCCGAAAGCACCAGATCATCCACACGGGAGAGAAGCCGTACAGA
  TGCAGTGTCTGTGGGAAGGCCTTCAGCCACAGCTCAGCCCTCATTCAGCACCAGGGCGTGCACACAGGCG
  ACAAGCCCTACGCCTGCCACGAGTGTGGGAAGACCTTTGGTCGCAGCTCCAACCTCATCCTTCACCAGCG
  AGTCCACACTGGAGAGAAGCCCTATGAATGTACTGAATGTGGAAAAACCTTCAGCCAGAGCTCAACCCTC
  ATTCAGCATCAGAGGATTCATAATGGGCTGAAGCCCCATGAATGTAACCAGTGTGGTAAAGCCTTCAACC
  GAAGCTCAAATCTCATTCACCACCAGAAAGTTCATACTGGGGAAAAACCCTACACCTGTGTTGAATGTGG
  TAAGGGCTTCAGCCAGAGCTCACACCTCATTCAGCATCAGATAATCCACACGGGCGAGCGCCCCTACAAA
  TGCAGTGAGTGTGGGAAAGCCTTCAGTCAGCGTTCGGTCCTCATCCAGCACCAGAGGATTCACACTGGGG
  TGAAGCCCTATGACTGTGCTGCTTGTGGGAAAGCCTTCAGCCAGCGATCAAAGTTGATCAAACACCAGTT
  GATTCACACCAGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSLRTRREEAEMELSVPGPSPWTPAAQARVRDAPAVTHPGSAACGTPCCSDTELEAICPHYQQPDCDTR
  TEDKEFLHKEDIHEDLESQAEISENYAGDVSQVPELGDLCDDVSERDWGVPEGRRLPQSLSQEGDFTPAA
  MGLLRGPLGEKDLDCNGFDSRFSLSPNLMACQEIPTEERPHPYDMGGQSFQHSVDLTGHEGVPTAESPLI
  CNECGKTFQGNPDLIQRQIVHTGEASFMCDDCGKTFSQNSVLKNRHRSHMSEKAYQCSECGKAFRGHSDF
  SRHQSHHSSERPYMCNECGKAFSQNSSLKKHQKSHMSEKPYECNECGKAFRRSSNLIQHQRIHSGEKPYV
  CSECGKAFRRSSNLIKHHRTHTGEKPFECGECGKAFSQSAHLRKHQRVHTGEKPYECNDCGKPFSRVSNL
  IKHHRVHTGEKPYKCSDCGKAFSQSSSLIQHRRIHTGEKPHVCNVCGKAFSYSSVLRKHQIIHTGEKPYR
  CSVCGKAFSHSSALIQHQGVHTGDKPYACHECGKTFGRSSNLILHQRVHTGEKPYECTECGKTFSQSSTL
  IQHQRIHNGLKPHECNQCGKAFNRSSNLIHHQKVHTGEKPYTCVECGKGFSQSSHLIQHQIIHTGERPYK
  CSECGKAFSQRSVLIQHQRIHTGVKPYDCAACGKAFSQRSKLIKHQLIHTRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006958
• ORF Size: 2046 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006958.3
• RefSeq Size: 2546 bp
• RefSeq ORF: 2049 bp
• Locus ID: 7564
• UniProt ID: P17020
• Cytogenetics: 8q24.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 76.5 kDa
• Gene Summary: The protein encoded by this gene contains multiple tandem zinc finger motifs. The encoded protein is involved in the differentiation of erythroid and megakaryocytic cells. This gene is located in a cluster of related genes on chromosome 8 encoding zinc finger proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]