GAL3ST4 (NM_024637) Human Tagged ORF Clone

CAT#: RC203401

GAL3ST4 (Myc-DDK-tagged)-Human galactose-3-O-sulfotransferase 4 (GAL3ST4)

Product Images

Circular map for RC203401

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  Western blot validation of overexpression lysate (Cat# LY411104) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203401 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GAL3ST4 protein (Cat# TP303401). The protein was produced from HEK293T cells transfected with GAL3ST4 cDNA clone (Cat# RC203401) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAL3ST4
• Synonyms: GAL3ST-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203401 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCTCTCTCTCCTGCCAGGACGCTGCGGCTCTGGGGACCTCGGAGCCTGGGGGTGGCTCTGGGAG
  TCTTCATGACCATTGGCTTTGCACTCCAGCTCTTGGGAGGGCCCTTCCAGAGGAGGCTACCTGGGCTACA
  GCTCCGACAGCCCTCGGCCCCATCCCTACGACCAGCCCTTCCGTCCTGCCCACCCCGGCAGCGACTGGTG
  TTCCTGAAGACACATAAATCCGGGAGCAGCTCTGTGCTGAGCCTGCTTCACCGCTATGGGGACCAGCACG
  GGCTGCGCTTCGCCCTCCCTGCCCGCTACCAGTTTGGCTACCCAAAGCTCTTCCAGGCCTCTAGGGTAAA
  AGGCTACCGCCCACAGGGTGGAGGCACCCAGCTCCCCTTCCACATCCTCTGTCACCACATGAGGTTCAAC
  CTGAAAGAGGTACTTCAGGTCATGCCTTCTGACAGCTTCTTTTTTTCCATTGTCCGAGACCCAGCGGCTC
  TGGCTCGCTCTGCCTTCTCCTACTATAAATCCACCTCATCAGCCTTCCGCAAGTCACCATCTTTGGCTGC
  CTTCCTGGCCAATCCTCGAGGCTTCTACAGGCCTGGGGCCCGTGGGGACCACTACGCTCGCAACTTACTA
  TGGTTTGACTTTGGCCTGCCCTTTCCCCCAGAGAAGAGGGCCAAGAGAGGGAATATTCATCCCCCCAGAG
  ACCCCAACCCCCCACAGCTGCAGGTCTTGCCTTCTGGTGCTGGCCCTCGAGCCCAAACCCTCAATCCCAA
  TGCCCTCATCCATCCTGTTTCCACTGTTACTGATCATCGCAGCCAGATATCAAGCCCTGCCTCTTTCGAT
  TTGGGGTCTTCATCCTTCATCCAGTGGGGTCTGGCCTGGCTGGACTCTGTCTTTGACCTGGTCATGGTGG
  CTGAGTACTTCGATGAGTCATTGGTTCTGCTGGCAGATGCCCTGTGCTGGGGTCTAGATGACGTGGTGGG
  CTTCATGCACAATGCCCAGGCTGGACATAAGCAGGGCCTCAGCACTGTCAGCAACAGTGGACTGACTGCG
  GAGGACCGGCAGCTGACTGCACGGGCCCGAGCCTGGAACAACCTGGACTGGGCTCTCTATGTCCACTTCA
  ACCGCAGTCTCTGGGCACGGATAGAGAAATACGGCCAGGGCCGGCTGCAGACAGCTGTGGCCGAGCTCCG
  GGCTCGCCGAGAGGCCCTAGCGAAACATTGTCTGGTAGGGGGTGAGGCTTCTGACCCCAAATACATCACT
  GATCGCCGGTTCCGCCCCTTCCAGTTTGGGTCAGCTAAGGTTTTGGGCTATATACTTCGGAGTGGATTGA
  GCCCCCAAGACCAAGAGGAATGTGAGCGCCTAGCTACCCCTGAGCTCCAGTACAAGGACAAGCTGGATGT
  CAAGCAGTTCCCCCCTACCGTCTCACTGCCCCTCAAGACTTCAAGGCCACTCTCCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203401 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPLSPARTLRLWGPRSLGVALGVFMTIGFALQLLGGPFQRRLPGLQLRQPSAPSLRPALPSCPPRQRLV
  FLKTHKSGSSSVLSLLHRYGDQHGLRFALPARYQFGYPKLFQASRVKGYRPQGGGTQLPFHILCHHMRFN
  LKEVLQVMPSDSFFFSIVRDPAALARSAFSYYKSTSSAFRKSPSLAAFLANPRGFYRPGARGDHYARNLL
  WFDFGLPFPPEKRAKRGNIHPPRDPNPPQLQVLPSGAGPRAQTLNPNALIHPVSTVTDHRSQISSPASFD
  LGSSSFIQWGLAWLDSVFDLVMVAEYFDESLVLLADALCWGLDDVVGFMHNAQAGHKQGLSTVSNSGLTA
  EDRQLTARARAWNNLDWALYVHFNRSLWARIEKYGQGRLQTAVAELRARREALAKHCLVGGEASDPKYIT
  DRRFRPFQFGSAKVLGYILRSGLSPQDQEECERLATPELQYKDKLDVKQFPPTVSLPLKTSRPLSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024637
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024637.2
• RefSeq Size: 2561 bp
• RefSeq ORF: 1461 bp
• Locus ID: 79690
• UniProt ID: Q96RP7
• Cytogenetics: 7q22.1
• Protein Families: Transmembrane
• MW: 54.2 kDa
• Gene Summary: This gene encodes a member of the galactose-3-O-sulfotransferase protein family. The product of this gene catalyzes sulfonation by transferring a sulfate to the C-3' position of galactose residues in O-linked glycoproteins. This enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6)GalNAc being good substrates. [provided by RefSeq, Jul 2008]