CAB39L (NM_030925) Human Tagged ORF Clone

CAT#: RC203394

CAB39L (Myc-DDK-tagged)-Human calcium binding protein 39-like (CAB39L), transcript variant 1

Product Images

Circular map for RC203394

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  Western blot validation of overexpression lysate (Cat# LY421532) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211881 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CAB39L protein (Cat# TP303394). The protein was produced from HEK293T cells transfected with CAB39L cDNA clone (Cat# RC203394) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CAB39L
• Synonyms: bA103J18.3; MO2L; MO25-BETA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203394 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAAATGCCTTTGTTTAGTAAATCACACAAAAATCCAGCAGAAATTGTGAAAATCCTGAAAGACA
  ATTTGGCCATTTTGGAAAAGCAAGACAAAAAGACAGACAAGGCTTCAGAAGAAGTGTCTAAATCACTGCA
  AGCAATGAAAGAAATTCTGTGTGGTACAAACGAGAAAGAACCCCCGACAGAAGCAGTGGCTCAGCTAGCA
  CAAGAACTCTACAGCAGTGGCCTGCTGGTGACACTGATAGCTGACCTGCAGCTGATAGACTTTGAGGAAA
  AAAAAGATGTGACCCAGATATTTAACAACATCTTGAGAAGACAGATAGGCACTCGGAGTCCTACTGTGGA
  GTATATTAGTGCTCATCCTCATATCCTGTTTATGCTCCTCAAAGGATATGAAGCCCCACAGATTGCCTTA
  CGTTGTGGGATTATGCTGAGAGAATGTATTCGACATGAACCACTTGCCAAAATCATCCTCTTTTCTAATC
  AATTCAGAGATTTCTTTAAGTACGTGGAGTTGTCAACATTTGATATTGCTTCAGATGCCTTTGCTACTTT
  CAAGGATTTACTAACCAGACATAAAGTGTTGGTAGCAGACTTCTTAGAACAAAATTACGACACTATTTTT
  GAAGACTATGAGAAATTGCTTCAGTCTGAGAATTATGTTACTAAGAGACAGTCTTTAAAGCTGCTAGGGG
  AGCTGATCCTGGACCGTCACAACTTTGCCATCATGACAAAGTATATCAGCAAGCCGGAGAACCTGAAACT
  CATGATGAACCTCCTTCGGGATAAAAGTCCCAACATCCAGTTTGAAGCCTTTCATGTTTTTAAGGTGTTT
  GTGGCCAGTCCTCACAAAACACAGCCTATTGTGGAGATCCTGTTAAAAAATCAGCCCAAACTCATTGAGT
  TTCTGAGCAGCTTCCAAAAAGAAAGGACGGATGATGAGCAGTTCGCTGACGAGAAGAACTACTTGATTAA
  ACAGATCCGAGACTTGAAGAAAACGGCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203394 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKMPLFSKSHKNPAEIVKILKDNLAILEKQDKKTDKASEEVSKSLQAMKEILCGTNEKEPPTEAVAQLA
  QELYSSGLLVTLIADLQLIDFEEKKDVTQIFNNILRRQIGTRSPTVEYISAHPHILFMLLKGYEAPQIAL
  RCGIMLRECIRHEPLAKIILFSNQFRDFFKYVELSTFDIASDAFATFKDLLTRHKVLVADFLEQNYDTIF
  EDYEKLLQSENYVTKRQSLKLLGELILDRHNFAIMTKYISKPENLKLMMNLLRDKSPNIQFEAFHVFKVF
  VASPHKTQPIVEILLKNQPKLIEFLSSFQKERTDDEQFADEKNYLIKQIRDLKKTAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030925
• ORF Size: 1011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030925.4
• RefSeq Size: 3631 bp
• RefSeq ORF: 1014 bp
• Locus ID: 81617
• UniProt ID: Q9H9S4
• Cytogenetics: 13q14.2
• Domains: Mo25
• Protein Pathways: mTOR signaling pathway
• MW: 39.2 kDa
• Gene Summary: Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1 (By similarity).[UniProtKB/Swiss-Prot Function]