MUS81 (NM_025128) Human Tagged ORF Clone

CAT#: RC203373

MUS81 (Myc-DDK-tagged)-Human MUS81 endonuclease homolog (S. cerevisiae) (MUS81)

Product Images

Circular map for RC203373

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MUS81 (Cat# RC203373, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MUS81 antibody (Cat# TA890060). Positive lysates LY403051 (100ug) and LC403051 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403051) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203373 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MUS81 protein (Cat# TP303373). The protein was produced from HEK293T cells transfected with MUS81 cDNA clone (Cat# RC203373) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MUS81
• Synonyms: SLX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203373 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCGGTCCGCCTGGGCCGGAAGCGCCCGCTGCCTGCCTGTCCCAACCCGCTCTTCGTTCGCT
  GGCTGACCGAGTGGCGGGACGAGGCGACCCGCAGCAGGCACCGCACGCGCTTCGTATTTCAGAAGGCGCT
  GCGTTCCCTCCGACGGTACCCACTGCCGCTGCGCAGCGGGAAGGAAGCTAAGATCCTACAGCACTTCGGA
  GACGGGCTCTGCCGGATGCTGGACGAGCGGCTGCAGCGGCACCGAACATCGGGCGGTGACCATGCCCCGG
  ACTCACCATCTGGAGAGAACAGTCCAGCCCCGCAGGGGCGACTTGCGGAAGTCCAGGACTCTTCCATGCC
  AGTTCCTGCCCAGCCCAAAGCGGGAGGCTCTGGCAGCTACTGGCCAGCTCGGCACTCAGGAGCCCGAGTG
  ATACTGCTGGTGCTCTACCGGGAGCACCTGAATCCTAATGGTCACCACTTCTTAACCAAGGAGGAGCTGC
  TGCAGAGGTGTGCTCAGAAGTCCCCCAGGGTAGCCCCTGGGAGTGCCCCACCCTGGCCAGCCCTCCGCTC
  CCTCCTTCACAGGAACCTGGTCCTCAGGACACACCAGCCAGCCAGGTACTCATTGACCCCAGAGGGCCTG
  GAGCTGGCCCAGAAGTTGGCCGAGTCAGAAGGCCTGAGCTTGCTGAATGTGGGCATCGGGCCCAAGGAGC
  CCCCTGGGGAGGAGACAGCAGTGCCAGGAGCAGCTTCAGCAGAGCTTGCCAGTGAAGCAGGGGTCCAGCA
  GCAGCCACTGGAGCTGAGGCCTGGAGAGTACAGGGTGCTGTTGTGTGTGGACATTGGCGAGACCCGGGGG
  GGCGGGCACAGGCCGGAGCTGCTCCGAGAGCTACAGCGGCTGCACGTGACCCACACGGTGCGCAAGCTGC
  ACGTTGGAGATTTTGTGTGGGTGGCTCAGGAGACCAATCCTAGAGACCCAGCAAACCCTGGGGAGTTGGT
  ACTGGATCACATTGTGGAGCGCAAGCGACTGGATGACCTTTGCAGCAGCATCATCGACGGCCGCTTCCGG
  GAGCAGAAGTTCCGACTGAAGCGCTGTGGTCTGGAGCGCCGGGTATACCTGGTGGAAGAGCATGGTTCCG
  TCCACAACCTCAGCCTTCCTGAGAGCACACTGCTGCAGGCTGTCACCAACACTCAGGTCATTGATGGCTT
  TTTTGTGAAGCGCACAGCAGACATTAAGGAGTCAGCCGCCTACCTGGCCCTCTTGACTCGGGGCCTGCAG
  AGACTCTACCAGGGCCACACCCTACGCAGCCGCCCCTGGGGAACCCCTGGGAACCCTGAATCAGGGGCCA
  TGACCTCTCCAAACCCTCTCTGCTCACTCCTCACCTTCAGTGACTTCAACGCAGGAGCCATCAAGAATAA
  GGCCCAGTCGGTGCGAGAAGTGTTTGCCCGGCAGCTGATGCAGGTGCGCGGAGTGAGTGGGGAGAAGGCA
  GCAGCCCTGGTGGATCGATACAGCACCCCTGCCAGCCTCCTGGCCGCCTATGATGCCTGTGCCACCCCCA
  AGGAACAAGAGACACTGCTGAGCACCATTAAGTGTGGGCGTCTACAGAGGAATCTGGGGCCTGCTCTGAG
  CAGGACCTTATCCCAGCTCTACTGCAGCTACGGCCCCTTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPVRLGRKRPLPACPNPLFVRWLTEWRDEATRSRHRTRFVFQKALRSLRRYPLPLRSGKEAKILQHFG
  DGLCRMLDERLQRHRTSGGDHAPDSPSGENSPAPQGRLAEVQDSSMPVPAQPKAGGSGSYWPARHSGARV
  ILLVLYREHLNPNGHHFLTKEELLQRCAQKSPRVAPGSAPPWPALRSLLHRNLVLRTHQPARYSLTPEGL
  ELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRG
  GGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFR
  EQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQ
  RLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKA
  AALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025128
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_025128.3, NP_079404.2
• RefSeq Size: 2406 bp
• RefSeq ORF: 1656 bp
• Locus ID: 80198
• UniProt ID: Q96NY9
• Cytogenetics: 11q13.1
• Domains: ERCC4
• Protein Pathways: Homologous recombination
• MW: 61.1 kDa
• Gene Summary: This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks. [provided by RefSeq, Jun 2017]