XTP4 (MIEN1) (NM_032339) Human Tagged ORF Clone

CAT#: RC203346

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MIEN1 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 37 (C17orf37)



Reconstitution Protocol

USD 150.00

USD 225.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MIEN1
Synonyms C17orf37; C35; ORB3; RDX12; XTP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203346 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGGGAGCCGGGGCAGACGTCCGTAGCGCCCCCTCCCGAGGAGGTCGAGCCGGGCAGTGGGGTCC
GCATCGTGGTGGAGTACTGTGAACCCTGCGGCTTCGAGGCGACCTACCTGGAGCTGGCCAGTGCTGTGAA
GGAGCAGTATCCGGGCATCGAGATCGAGTCGCGCCTCGGGGGCACAGGTGCCTTTGAGATAGAGATAAAT
GGACAGCTGGTGTTCTCCAAGCTGGAGAATGGGGGCTTTCCCTATGAGAAAGATCTCATTGAGGCCATCC
GAAGAGCCAGTAATGGAGAAACCCTAGAAAAGATCACCAACAGCCGTCCTCCCTGCGTCATCCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203346 protein sequence
Red=Cloning site Green=Tags(s)

MSGEPGQTSVAPPPEEVEPGSGVRIVVEYCEPCGFEATYLELASAVKEQYPGIEIESRLGGTGAFEIEIN
GQLVFSKLENGGFPYEKDLIEAIRRASNGETLEKITNSRPPCVIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032339
ORF Size 345 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_032339.5
RefSeq Size 781 bp
RefSeq ORF 348 bp
Locus ID 84299
UniProt ID Q9BRT3
Cytogenetics 17q12
MW 12.4 kDa
Gene Summary Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.[UniProtKB/Swiss-Prot Function]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.