APIP (NM_015957) Human Tagged ORF Clone
CAT#: RC203297
APIP (Myc-DDK-tagged)-Human APAF1 interacting protein (APIP)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_015957" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | APIP |
Synonyms | APIP2; CGI-29; CGI29; hAPIP; MMRP19 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203297 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCTGTGATGCTCGGGAGGGAGACTGTTGTTCCCGGAGATGCGGCGCGCAGGACAAGGAGCGTC CAAGATACCTGATCCCAGAACTTTGCAAACAGTTTTACCATTTAGGCTGGGTCACTGGGACTGGAGGAGG AATTAGCTTGAAGCATGGCGATGAAATCTACATTGCTCCTTCAGGAGTGCAAAAGGAACGAATTCAGCCT GAAGACATGTTTGTTTGTGATATAAATGAAAAGGACATAAGTGGACCTTCGCCATCGAAGAAGCTAAAAA AAAGCCAGTGTACTCCTCTTTTCATGAATGCTTACACAATGAGAGGAGCAGGTGCAGTGATTCATACCCA CTCTAAAGCTGCTGTGATGGCCACACTTCTCTTTCCAGGACGGGAGTTTAAAATTACACATCAAGAGATG ATAAAAGGAATAAAGAAATGTACTTCCGGAGGGTATTATAGATATGATGATATGTTAGTGGTACCCATTA TTGAGAATACACCTGAGGAGAAAGACCTCAAAGATAGAATGGCTCATGCAGTGAATGAATACCCAGACTC CTGTGCAGTACTGGTCAGACGTCATGGAGTATATGTGTGGGGGGAAACATGGGAGAAGGCCAAAACCATG TGTGAGTGTTATGACTATTTATTTGATATTGCCGTATCAATGAAGAAAGTAGGACTTGATCCTTCACAGC TCCCAGTTGGAGAAAATGGAATTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203297 protein sequence
Red=Cloning site Green=Tags(s) MSGCDAREGDCCSRRCGAQDKERPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQP EDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEM IKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAVNEYPDSCAVLVRRHGVYVWGETWEKAKTM CECYDYLFDIAVSMKKVGLDPSQLPVGENGIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_015957 |
ORF Size | 726 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015957.4 |
RefSeq Size | 1285 bp |
RefSeq ORF | 729 bp |
Locus ID | 51074 |
UniProt ID | Q96GX9 |
Cytogenetics | 11p13 |
Domains | Aldolase_II |
Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
MW | 27.1 kDa |
Gene Summary | APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203297L3 | Lenti ORF clone of Human APAF1 interacting protein (APIP), Myc-DDK-tagged |
USD 600.00 |
|
RC203297L4 | Lenti ORF clone of Human APAF1 interacting protein (APIP), mGFP tagged |
USD 600.00 |
|
RG203297 | APIP (tGFP-tagged) - Human APAF1 interacting protein (APIP) |
USD 500.00 |
|
SC319694 | APIP (untagged)-Human APAF1 interacting protein (APIP) |
USD 300.00 |
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