HSD17B2 (NM_002153) Human Tagged ORF Clone

CAT#: RC203293

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HSD17B2 (Myc-DDK-tagged)-Human hydroxysteroid (17-beta) dehydrogenase 2 (HSD17B2)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HSD17B2
Synonyms EDH17B2; HSD17; SDR9C2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203293 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCACTTTCTTCTCGGACACAGCATGGATCTGCCTGGCTGTCCCCACAGTACTATGTGGGACAGTAT
TTTGCAAATACAAGAAGAGCTCAGGGCAGCTGTGGAGCTGGATGGTCTGCCTGGCAGGCCTCTGTGCAGT
CTGCCTGCTCATCCTGTCCCCTTTTTGGGGCTTGATCCTCTTCTCGGTGTCATGCTTCCTCATGTATACT
TACTTATCTGGCCAAGAATTGTTACCTGTGGATCAGAAGGCAGTCCTGGTGACAGGTGGTGATTGCGGGC
TTGGCCATGCTTTGTGCAAGTATCTGGATGAGCTGGGCTTCACGGTATTTGCCGGAGTTTTGAATGAAAA
TGGCCCAGGAGCTGAGGAATTGCGAAGAACCTGCTCTCCGCGCCTCTCGGTGCTCCAAATGGACATCACG
AAGCCAGTGCAGATAAAAGATGCTTACAGCAAGGTTGCAGCAATGCTGCAGGACAGAGGACTGTGGGCTG
TGATCAACAATGCTGGGGTGCTTGGCTTTCCAACTGATGGGGAGCTTCTTCTTATGACTGACTACAAACA
ATGCATGGCCGTGAACTTCTTTGGAACTGTGGAGGTCACAAAGACGTTTTTGCCTCTTCTTAGAAAATCC
AAAGGGAGGCTGGTGAATGTCAGCAGCATGGGAGGAGGGGCCCCAATGGAAAGGCTGGCATCTTATGGCT
CATCAAAGGCGGCTGTGACCATGTTCTCATCAGTTATGAGACTGGAGCTTTCCAAGTGGGGAATTAAAGT
TGCTTCCATCCAACCTGGAGGCTTCCTAACAAATATCGCAGGCACCAGTGACAAGTGGGAAAAGCTGGAG
AAGGACATTCTGGACCACCTCCCCGCTGAGGTACAGGAAGACTACGGCCAGGACTACATCTTAGCACAGC
GGAATTTCCTCCTATTGATCAACTCGTTAGCCAGCAAGGACTTCTCTCCGGTGCTGCGGGACATCCAGCA
TGCTATCTTGGCGAAGAGCCCTTTTGCCTATTACACGCCAGGGAAAGGCGCTTACTTGTGGATCTGCCTT
GCTCACTATTTGCCTATTGGCATATATGATTACTTTGCTAAAAGACATTTTGGCCAAGACAAGCCCATGC
CCAGAGCTCTAAGAATGCCTAACTACAAGAAAAAGGCCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203293 protein sequence
Red=Cloning site Green=Tags(s)

MSTFFSDTAWICLAVPTVLCGTVFCKYKKSSGQLWSWMVCLAGLCAVCLLILSPFWGLILFSVSCFLMYT
YLSGQELLPVDQKAVLVTGGDCGLGHALCKYLDELGFTVFAGVLNENGPGAEELRRTCSPRLSVLQMDIT
KPVQIKDAYSKVAAMLQDRGLWAVINNAGVLGFPTDGELLLMTDYKQCMAVNFFGTVEVTKTFLPLLRKS
KGRLVNVSSMGGGAPMERLASYGSSKAAVTMFSSVMRLELSKWGIKVASIQPGGFLTNIAGTSDKWEKLE
KDILDHLPAEVQEDYGQDYILAQRNFLLLINSLASKDFSPVLRDIQHAILAKSPFAYYTPGKGAYLWICL
AHYLPIGIYDYFAKRHFGQDKPMPRALRMPNYKKKAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002153
ORF Size 1161 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002153.3
RefSeq Size 1451 bp
RefSeq ORF 1164 bp
Locus ID 3294
UniProt ID P37059
Cytogenetics 16q23.3
Domains adh_short
Protein Families Druggable Genome, Transmembrane
Protein Pathways Androgen and estrogen metabolism, Metabolic pathways
MW 42.8 kDa
Gene Summary Capable of catalyzing the interconversion of testosterone and androstenedione, as well as estradiol and estrone. Also has 20-alpha-HSD activity. Uses NADH while EDH17B3 uses NADPH.[UniProtKB/Swiss-Prot Function]
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.