FMO2 (NM_001460) Human Tagged ORF Clone

CAT#: RC203237

FMO2 (Myc-DDK-tagged)-Human flavin containing monooxygenase 2 (non-functional) (FMO2)

Product Images

Circular map for RC203237

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  Western blot validation of overexpression lysate (Cat# LY400568) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203237 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FMO2 protein (Cat# TP303237). The protein was produced from HEK293T cells transfected with FMO2 cDNA clone (Cat# RC203237) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FMO2
• Synonyms: FMO1B1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203237 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGCTGTGTGGATG
  AGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTGTGGAGGTTCAAAGAGAATGT
  GGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACCAACACCAGCAAAGAAATGTCCTGTTTCAGT
  GACTTTCCAATGCCTGAAGATTTTCCAAACTTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTT
  TTGCTAAAAAATTTGATCTGCTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCC
  AGATTTCTCATCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT
  GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTTCCAGGTATGG
  AGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGATGGATTTGAGGGAAAACGCAT
  CCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCTGTTGAGCTGAGTAAGAATGCTGCTCAGGTT
  TTTATCAGCACCAGGCATGGCACCTGGGTCATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAG
  TGTTCCACACCCGGTTTCGTTCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGA
  ACAACAGATGAATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG
  GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAATCTACAGTGA
  AAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAGAACATTGATGTCATCATTTT
  TGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGATTCACTCGTTAAAGTAGAGAATAATATGGTC
  TCACTGTATAAATACATATTCCCCGCTCACCTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGC
  CCCTAGGTTCCATTTTCCCAACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTG
  TAGCCTGCCCTCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT
  GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCCTTAGAGATAG
  GTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCTGTGAGACTCTATTTCGGACC
  CTGCAACTCCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203237 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNTSKEMSCFS
  DFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFSSSGQWKVVTQSNGKEQSAVF
  DAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPDGFEGKRILVIGMGNSGSDIAVELSKNAAQV
  FISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMK
  EPVLNDDVPSRLLCGAIKVKSTVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMV
  SLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERTMMMDIIKRNEKRIDLF
  GESQSQTLQTNYVDYLDELALEIGAKPDFCSLLFKDPKLAVRLYFGPCNSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001460
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001460.3
• RefSeq Size: 5304 bp
• RefSeq ORF: 1608 bp
• Locus ID: 2327
• UniProt ID: Q99518
• Cytogenetics: 1q24.3
• Protein Pathways: Drug metabolism - cytochrome P450
• MW: 53.6 kDa
• Gene Summary: This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]