ACAD9 (NM_014049) Human Tagged ORF Clone

CAT#: RC203235

ACAD9 (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase family, member 9 (ACAD9), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ACAD9 (Cat# RC203235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAD9 (Cat# TA811802)(1:2000). Positive lysates LY402280 (100ug) and LC402280 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402280) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203235 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACAD9 protein (Cat# TP303235). The protein was produced from HEK293T cells transfected with ACAD9 cDNA clone (Cat# RC203235) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACAD9
• Synonyms: MC1DN20; NPD002
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203235 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCTGCGGGCTCTTCCTGCGCACCACGGCTGCGGCTCGTGCCTGCCGGGGTCTGGTGGTCTCTA
  CCGCGAACCGGCGGCTACTGCGCACCAGCCCGCCTGTACGAGCTTTCGCCAAAGAGCTTTTCCTAGGCAA
  AATCAAGAAGAAAGAAGTTTTCCCATTTCCAGAAGTTAGCCAAGATGAACTTAATGAAATCAATCAGTTC
  TTGGGACCCGTGGAAAAATTCTTCACTGAAGAGGTGGACTCCCGAAAAATTGACCAGGAAGGGAAAATCC
  CAGATGAAACTTTGGAGAAATTGAAGAGCCTAGGGCTTTTTGGGCTGCAAGTCCCAGAAGAATATGGTGG
  CCTGGGCTTCTCCAACACCATGTACTCACGACTAGGGGAGATCATCAGCATGGATGGGTCCATCACTGTG
  ACCCTGGCAGCGCACCAGGCTATTGGCCTCAAGGGGATCATCTTGGCTGGCACTGAGGAGCAGAAAGCCA
  AATACTTGCCTAAACTGGCGTCCGGGGAGCACATTGCAGCCTTCTGCCTCACGGAGCCAGCCAGTGGGAG
  CGATGCAGCCTCAATCCGGAGCAGAGCCACACTAAGTGAAGACAAGAAGCACTACATCCTCAATGGCTCC
  AAGGTCTGGATTACTAATGGAGGACTGGCCAATATTTTTACTGTGTTTGCAAAGACTGAGGTCGTTGATT
  CTGATGGATCAGTGAAAGACAAAATCACAGCATTCATAGTAGAAAGAGACTTTGGTGGAGTCACTAATGG
  GAAACCCGAAGATAAATTAGGCATTCGGGGCTCCAACACTTGTGAAGTCCATTTTGAAAACACCAAGATA
  CCTGTGGAAAACATCCTTGGAGAGGTCGGAGATGGGTTTAAGGTGGCCATGAACATCCTCAACAGCGGCC
  GGTTCAGCATGGGCAGCGTCGTGGCTGGGCTGCTCAAGAGATTGATTGAAATGACTGCTGAGTACGCCTG
  CACAAGGAAACAGTTTAACAAGAGGCTCAGTGAATTTGGATTGATTCAGGAGAAATTTGCACTGATGGCT
  CAGAAGGCTTACGTCATGGAGAGTATGACCTACCTCACAGCAGGGATGCTGGACCAACCTGGCTTTCCCG
  ACTGCTCCATCGAGGCAGCCATGGTGAAGGTGTTCAGCTCCGAGGCCGCCTGGCAGTGTGTGAGTGAGGC
  GCTGCAGATCCTCGGGGGCTTGGGCTACACAAGGGACTATCCGTACGAGCGCATACTGCGTGACACCCGC
  ATCCTCCTCATCTTCGAGGGAACCAATGAGATTCTCCGGATGTACATCGCCCTGACGGGTCTGCAGCATG
  CCGGCCGCATCCTGACTACCAGGATCCATGAGCTTAAACAGGCCAAAGTGAGCACAGTCATGGATACCGT
  TGGCCGGAGGCTTCGGGACTCCCTGGGCCGAACTGTGGACCTGGGGCTGACAGGCAACCATGGAGTTGTG
  CACCCTAGTCTTGCGGACAGTGCCAACAAGTTTGAGGAGAACACCTACTGCTTCGGCCGGACCGTGGAGA
  CACTGCTGCTCCGCTTTGGCAAGACCATCATGGAGGAGCAGCTGGTACTGAAGCGGGTGGCCAACATCCT
  CATCAACCTGTATGGCATGACGGCCGTGCTGTCGCGGGCCAGCCGCTCCATCCGCATTGGGCTCCGCAAC
  CACGACCACGAGGTTCTCTTGGCCAACACCTTCTGCGTGGAAGCTTACTTGCAGAATCTCTTCAGCCTCT
  CTCAGCTGGACAAGTATGCTCCAGAAAACCTAGATGAGCAGATTAAGAAAGTGTCCCAGCAGATCCTTGA
  GAAGCGAGCCTATATCTGTGCCCACCCTCTGGACAGGACATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203235 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGCGLFLRTTAAARACRGLVVSTANRRLLRTSPPVRAFAKELFLGKIKKKEVFPFPEVSQDELNEINQF
  LGPVEKFFTEEVDSRKIDQEGKIPDETLEKLKSLGLFGLQVPEEYGGLGFSNTMYSRLGEIISMDGSITV
  TLAAHQAIGLKGIILAGTEEQKAKYLPKLASGEHIAAFCLTEPASGSDAASIRSRATLSEDKKHYILNGS
  KVWITNGGLANIFTVFAKTEVVDSDGSVKDKITAFIVERDFGGVTNGKPEDKLGIRGSNTCEVHFENTKI
  PVENILGEVGDGFKVAMNILNSGRFSMGSVVAGLLKRLIEMTAEYACTRKQFNKRLSEFGLIQEKFALMA
  QKAYVMESMTYLTAGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGLGYTRDYPYERILRDTR
  ILLIFEGTNEILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVV
  HPSLADSANKFEENTYCFGRTVETLLLRFGKTIMEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLRN
  HDHEVLLANTFCVEAYLQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014049
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014049.3, NP_054768.2
• RefSeq Size: 2608 bp
• RefSeq ORF: 1866 bp
• Locus ID: 28976
• UniProt ID: Q9H845
• Cytogenetics: 3q21.3
• Domains: Acyl-CoA_dh, Acyl-CoA_dh_M, Acyl-CoA_dh_N
• MW: 68.8 kDa
• Gene Summary: This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]