PSMD2 (NM_002808) Human Tagged ORF Clone

CAT#: RC203204

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PSMD2 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2)



Reconstitution Protocol

USD 831.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PSMD2
Synonyms P97; RPN1; S2; TRAP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203204 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGGAGGCCGGGACAAGGCGCCGGTGCAGCCCCAGCAGTCTCCAGCGGCGGCCCCCGGCGGCA
CGGACGAGAAGCCGAGCGGCAAGGAGCGGCGGGATGCCGGGGACAAGGACAAAGAACAGGAGCTGTCTGA
AGAGGATAAACAGCTTCAAGATGAACTGGAGATGCTCGTGGAACGACTAGGGGAGAAGGATACATCCCTG
TATCGACCAGCGCTGGAGGAATTGCGAAGGCAGATTCGTTCTTCTACAACTTCCATGACTTCAGTGCCCA
AGCCTCTCAAATTTCTGCGTCCACACTATGGCAAACTGAAGGAAATCTATGAGAACATGGCCCCTGGGGA
GAATAAGCGTTTTGCTGCTGACATCATCTCCGTTTTGGCCATGACCATGAGTGGGGAGCGTGAGTGCCTC
AAGTATCGGCTAGTGGGCTCCCAGGAGGAATTGGCATCATGGGGTCATGAGTATGTCAGGCATCTGGCAG
GAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAGCGGGAGCCTCTGCTCACTCT
GGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCAGAGCATGAGGCTTGCGACCTGCTTATGGAA
ATTGAGCAGGTGGACATGCTGGAGAAGGACATTGATGAAAATGCATATGCAAAGGTCTGCCTTTATCTCA
CCAGTTGTGTGAATTACGTGCCTGAGCCTGAGAACTCAGCCCTACTGCGTTGTGCCCTGGGTGTGTTCCG
AAAGTTTAGCCGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGACATGGAGTTGGTAGAAGAC
ATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATGCTAGGCCGGCATGGGGTGT
TCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATCATGTCCAATGTACAGCTCAA
CAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATGGAGCCCAAGGTGCCTGATGACATCTACAAA
ACCCACCTAGAGAACAACAGGTTTGGGGGCAGTGGCTCTCAGGTGGACTCTGCCCGCATGAACCTGGCCT
CCTCTTTTGTGAATGGCTTTGTGAATGCAGCTTTTGGCCAAGACAAGCTGCTAACAGATGATGGCAACAA
ATGGCTTTACAAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCATCTCTTGGGATGATTCTGCTGTGG
GATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCCTCTGAGGACTACATTAAGTCAGGAG
CTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAGTGTGACCCTGCTCTGGCACTGCTCTC
AGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGTTCCATCTTTGGGCTAGGCTTGGCTTATGCT
GGCTCAAATCGTGAAGATGTCCTAACACTGCTGCTGCCTGTGATGGGAGATTCAAAGTCCAGCATGGAGG
TGGCAGGTGTCACAGCTTTAGCCTGTGGAATGATAGCAGTAGGGTCCTGCAATGGAGATGTAACTTCCAC
TATCCTTCAGACCATCATGGAGAAGTCAGAGACTGAGCTCAAGGATACTTATGCTCGTTGGCTTCCTCTT
GGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATCGAGGCAATCCTGGCTGCACTGGAGGTTGTGT
CAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGATGTGTGTGCATATGCAGGCTCTGGGAATGTGCT
GAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACACTTTGACTCCAAAGAGAAGGAGGAAGACAAAGAC
AAGAAGGAAAAGAAAGACAAGGACAAGAAGGAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTGGCTG
TTCTGGGGATTGCCCTTATTGCTATGGGGGAGGAGATTGGTGCAGAGATGGCATTACGAACCTTTGGCCA
CTTGCTGAGATATGGGGAGCCTACACTCCGGAGGGCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCA
AATCCACGACTCAACATCCTGGATACCCTAAGCAAATTCTCTCATGATGCTGATCCAGAAGTTTCCTATT
ACTCCATTTTTGCCATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGTCTGGCTGCAATGCTGCGCCA
GTTAGCTCAATATCATGCCAAGGACCCAAACAACCTCTTCATGGTGCGCTTGGCACAGGGCCTGACACAT
TTAGGGAAGGGCACCCTTACCCTCTGCCCCTACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGG
CTGGACTGCTCACTGTGCTTGTCTCTTTCCTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGT
ATTGTATGGGCTGGTGGCTGCCATGCAGCCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTG
CCAGTGTCTGTCCGTGTGGGCCAGGCAGTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAGACTATCACAG
GGTTCCAGACGCATACAACCCCAGTGTTGTTGGCCCACGGGGAACGGGCAGAATTGGCCACTGAGGAGTT
TCTTCCTGTTACCCCCATTCTGGAAGGTTTTGTTATCCTTCGGAAGAACCCCAATTATGATCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203204 protein sequence
Red=Cloning site Green=Tags(s)

MEEGGRDKAPVQPQQSPAAAPGGTDEKPSGKERRDAGDKDKEQELSEEDKQLQDELEMLVERLGEKDTSL
YRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKRFAADIISVLAMTMSGERECL
KYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLME
IEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVED
IFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYK
THLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYA
GSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPL
GLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKD
KKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVS
NPRLNILDTLSKFSHDADPEVSYYSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTH
LGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002808
ORF Size 2724 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002808.2
RefSeq Size 3076 bp
RefSeq ORF 2727 bp
Locus ID 5708
UniProt ID Q13200
Cytogenetics 3q27.1
Domains PC_rep
Protein Families Druggable Genome
Protein Pathways Proteasome
MW 100.2 kDa
Gene Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
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