BHMT (NM_001713) Human Tagged ORF Clone

CAT#: RC203148

BHMT (Myc-DDK-tagged)-Human betaine--homocysteine S-methyltransferase (BHMT)

Product Images

Circular map for RC203148

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BHMT (Cat# RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT(Cat# TA500961). Positive lysates LY400644 (100ug) and LC400644 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203148 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BHMT protein (Cat# TP303148). The protein was produced from HEK293T cells transfected with BHMT cDNA clone (Cat# RC203148) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BHMT
• Synonyms: BHMT1; HEL-S-61p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203148 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCGTTGGGGGCAAAAAGGCCAAGAAGGGCATCCTAGAACGTTTAAATGCTGGAGAGATTGTGA
  TTGGAGATGGAGGGTTTGTCTTTGCACTGGAGAAGAGGGGCTACGTAAAGGCAGGACCCTGGACTCCTGA
  AGCTGCTGTGGAGCACCCAGAAGCAGTTCGCCAGCTTCATCGAGAGTTCCTCAGAGCTGGCTCAAACGTC
  ATGCAGACCTTCACCTTCTATGCGAGTGAAGACAAGCTGGAGAACAGGGGCAACTATGTCTTAGAGAAGA
  TATCTGGGCAGGAAGTCAATGAAGCTGCTTGCGACATCGCCCGACAAGTGGCTGATGAAGGAGATGCTTT
  GGTAGCAGGAGGAGTGAGTCAGACACCTTCATACCTTAGCTGCAAGAGTGAAACTGAAGTCAAAAAAGTA
  TTTCTGCAACAGTTAGAGGTCTTTATGAAGAAGAACGTGGACTTCTTGATTGCAGAGTATTTTGAACACG
  TTGAAGAAGCTGTGTGGGCAGTTGAAACCTTGATAGCATCCGGTAAACCTGTGGCAGCAACCATGTGCAT
  TGGCCCAGAAGGAGATTTGCATGGCGTGCCCCCCGGCGAGTGTGCAGTGCGCCTGGTGAAAGCAGGAGCA
  TCCATCATTGGTGTGAACTGCCACTTTGACCCCACCATTAGTTTAAAAACAGTGAAGCTCATGAAGGAGG
  GCTTGGAGGCTGCCCGACTGAAAGCTCACCTGATGAGCCAGCCCTTGGCTTACCACACTCCTGACTGCAA
  CAAGCAGGGATTCATCGATCTCCCAGAATTCCCATTTGGACTGGAACCCAGAGTTGCCACCAGATGGGAT
  ATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGGTCAGGTACATTGGCGGGTGCTGTGGATTTGAGC
  CCTACCACATCAGGGCAATTGCAGAGGAGCTGGCCCCAGAAAGGGGCTTTTTGCCACCAGCTTCAGAAAA
  ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGGTTAGAGCAAGGGCCAGGAAGGAA
  TACTGGGAGAATCTTCGGATAGCCTCAGGCCGGCCATACAACCCTTCAATGTCAAAGCCAGATGGCTGGG
  GAGTGACCAAAGGAACAGCCGAGCTGATGCAGCAGAAAGAAGCCACAACTGAGCAGCAGCTGAAAGAGCT
  CTTTGAAAAACAAAAATTCAAATCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203148 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNV
  MQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKV
  FLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGA
  SIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWD
  IQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKE
  YWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001713
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001713.3
• RefSeq Size: 2515 bp
• RefSeq ORF: 1221 bp
• Locus ID: 635
• UniProt ID: Q93088
• Cytogenetics: 5q14.1
• Domains: S-methyl_trans
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• MW: 45 kDa
• Gene Summary: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]