Nuclear Matrix Protein p84 (THOC1) (NM_005131) Human Tagged ORF Clone

CAT#: RC203117

THOC1 (Myc-DDK-tagged)-Human THO complex 1 (THOC1)

Product Images

Circular map for RC203117

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  Western blot validation of overexpression lysate (Cat# LY401574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203117 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified THOC1 protein (Cat# TP303117). The protein was produced from HEK293T cells transfected with THOC1 cDNA clone (Cat# RC203117) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nuclear Matrix Protein p84
• Synonyms: HPR1; P84; P84N5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203117 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCTACCAGAGAGG
  CCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTACCTGGCAGTGAAAATGAAAA
  AAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAAGAAGAAATTATAAATCATTCATCATGTGAA
  AACGTTTTAGCTATTATTTCTCTTGCTATTGGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTT
  TTGTATTGTTGGGAGATGTTTTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGA
  AAAAAATGTTGCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
  AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAGCTCTTTTTGG
  CCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAGTTTAATCTGGAAAATGTCAC
  TGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAGAAGCACACTGAAGATAGAGAAGAAGGAATG
  GATGTAGAAGAAGGCGAAATGGGAGATGAGGAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACC
  TGTATCGAAAATTCTGGTCACTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATG
  GAAAACTTTTCTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
  GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAATTTTTAACAA
  GTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACACATCCTGTTGCAGTATCTCAT
  TTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGTTCAAACTATGTTTTAACTGATGAGCAATCA
  CTTTGGATTGAAGATACTACAAAATCAGTTTATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGAT
  TTTCAAAGATGGTAGAGCATATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCC
  AAGTTTTGTGAAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
  GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGGCTTTGGAATC
  TTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATGCCCACTTTGGAGGAATTCTT
  TGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTGGAAAATGAATATAAGGCTGTGAACAATTCA
  AATTATGGTTGGAGAGCCCTGAGACTATTAGCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGC
  AGTTTAAAAGTTTACCAGAATATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTC
  TGAAGAAATAAAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
  CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAGCTGGGTGAAC
  AATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGGCAGATTGAGTGTGACAGTGA
  AGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGGCAAGATCAAGAGGGAGTTCATGCAACACCT
  GAGAATCTGATTAATGCACTGAATAAGTCTGGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATG
  AGACAAATAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203117 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPTPPLFSLPEARTRFTKSTREALNNKNIKPLLSTFSQVPGSENEKKCTLDQAFRGILEEEIINHSSCE
  NVLAIISLAIGGVTEGICTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNVATWKSNTFYSAGKNYLLRMC
  NDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNEQESTLGQKHTEDREEGM
  DVEEGEMGDEEAPTTCSIPIDYNLYRKFWSLQDYFRNPVQCYEKISWKTFLKYSEEVLAVFKSYKLDDTQ
  ASRKKMEELKTGGEHVYFAKFLTSEKLMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQS
  LWIEDTTKSVYQLLSENPPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERTSDTKPTRIIRKRTAPE
  DFLGKGPTKKILMGNEELTRLWNLCPDNMEACKSETREHMPTLEEFFEEAIEQADPENMVENEYKAVNNS
  NYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLKENES
  PDVRRDKPVTGEQIEVFANKLGEQWKILAPYLEMKDSEIRQIECDSEDMKMRAKQLLVAWQDQEGVHATP
  ENLINALNKSGLSDLAESLTNDNETNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005131
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005131.3
• RefSeq Size: 2133 bp
• RefSeq ORF: 1974 bp
• Locus ID: 9984
• UniProt ID: Q96FV9
• Cytogenetics: 18p11.32
• Domains: DEATH
• Protein Pathways: Proteasome, Spliceosome
• MW: 75.7 kDa
• Gene Summary: HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]