HSD11B1 (NM_005525) Human Tagged ORF Clone
HSD11B1 (Myc-DDK-tagged)-Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1
|Type||Human Tagged ORF Clone|
|Synonyms||11-beta-HSD1; 11-DH; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC203109 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC203109 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||876 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1477 bp|
|RefSeq ORF||879 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways|
|Gene Summary||The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]|
|cDNA Clone Resources|
|RC203109L1||Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1, Myc-DDK-tagged||
|RC203109L2||Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1, mGFP tagged||
|RC203109L3||Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1, Myc-DDK-tagged||
|RC203109L4||Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1, mGFP tagged||
|RG203109||HSD11B1 (tGFP-tagged) - Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1||
|SC109325||HSD11B1 (untagged)-Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1||
|SC321065||HSD11B1 (untagged)-Human hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), transcript variant 1||