MGC13096 (PDCD2L) (NM_032346) Human Tagged ORF Clone

CAT#: RC202997

PDCD2L (Myc-DDK-tagged)-Human programmed cell death 2-like (PDCD2L)

Product images

Western blot validation of overexpression lysate (Cat# LY410183) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202997 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PDCD2L protein (Cat# TP302997). The protein was produced from HEK293T cells transfected with PDCD2L cDNA clone (Cat# RC202997) using MegaTran 2.0 (Cat# TT210002).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDCD2L (Cat# RC202997, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD2L (Cat# TA812472)(1:500). Positive lysates LY410183 (100ug) and LC410183 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDCD2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202997 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTTCTGAAGCCGGTGCTGCTGGGCCTTCGAGATGCGCCGGTGCACGGCAGCCCCACAGGGC
  CGGGTGCCTGGACTGCTAGCAAGCTGGGCGGCATTCCGGATGCTCTGCCCACCGTGGCTGCGCCCAGGCC
  CGTGTGTCAGCGCTGCGGGCAGCCGCTCGCTCTGGTCGTGCAGGTGTATTGCCCGCTGGAAGGCTCCCCG
  TTTCACCGTCTGCTGCACGTGTTCGCGTGCGCCTGCCCCGGCTGTAGCACCGGCGGTGCGCGCAGCTGGA
  AGGTGTTCCGCTCCCAGTGCCTGCAGGTGCCAGAGAGAGAGGCGCAGGACGCTCAGAAACAGGGAAACAG
  CCTTGCAGCTGAGGACTGGTGTGAAGGTGCTGATGACTGGGGAAGTGATACTGAGGAGGGGCCTTCACCA
  CAGTTTACCTTGGATTTTGGGAATGATGCCAGCAGTGCCAAAGACGTAGACTGGACTGCTCGGCTCCAAG
  ACCTCCGCCTGCAGGATGCTGTCCTGGGTGCTGCCCATCCTGTGCCTCCTGGGCTGCCGCTCTTCCTGCC
  CTACTACATCTGTGTTGCAGATGAGGATGATTACAGGGACTTTGTCAACCTGGATCATGCCCACAGCCTT
  CTGAGGGACTATCAGCAGAGAGAAGGCATTGCCATGGATCAGTTGCTTTCCCAAAGCCTTCCTAATGATG
  GTGATGAAAAATATGAGAAGACCATAATTAAAAGTGGAGATCAGACGTTTTACAAATTCATGAAGCGAAT
  TGCTGCTTGTCAGGAGCAGATTTTGAGGTATTCCTGGAGTGGAGAGCCACTCTTTTTGACCTGCCCTACA
  TCAGAAGTCACCGAGCTCCCAGCCTGCAGCCAGTGTGGAGGCCAAAGGATATTTGAGTTTCAGCTTATGC
  CAGCACTGGTCAGCATGCTCAAGAGTGCTAATTTAGGTCTTTCTGTGGAATTTGGAACAATTCTAGTTTA
  CACATGTGAGAAGAGTTGCTGGCCCCCAAATCATCAGACTCCCATGGAAGAATTTTGTATTATACAAGAA
  GACCCAGATGAATTATTGTTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202997 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVLKPVLLGLRDAPVHGSPTGPGAWTASKLGGIPDALPTVAAPRPVCQRCGQPLALVVQVYCPLEGSP
  FHRLLHVFACACPGCSTGGARSWKVFRSQCLQVPEREAQDAQKQGNSLAAEDWCEGADDWGSDTEEGPSP
  QFTLDFGNDASSAKDVDWTARLQDLRLQDAVLGAAHPVPPGLPLFLPYYICVADEDDYRDFVNLDHAHSL
  LRDYQQREGIAMDQLLSQSLPNDGDEKYEKTIIKSGDQTFYKFMKRIAACQEQILRYSWSGEPLFLTCPT
  SEVTELPACSQCGGQRIFEFQLMPALVSMLKSANLGLSVEFGTILVYTCEKSCWPPNHQTPMEEFCIIQE
  DPDELLFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032346
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032346.2
• RefSeq Size: 1174 bp
• RefSeq ORF: 1077 bp
• Locus ID: 84306
• UniProt ID: Q9BRP1
• Cytogenetics: 19q13.11
• MW: 39.4 kDa
• Gene Summary: Over-expression suppresses AP1, CREB, NFAT, and NF-kB transcriptional activation, and delays cell cycle progression at S phase.[UniProtKB/Swiss-Prot Function]