Nuclear Factor Erythroid Derived 2 (NFE2) (NM_006163) Human Tagged ORF Clone

CAT#: RC202924

NFE2 (Myc-DDK-tagged)-Human nuclear factor (erythroid-derived 2), 45kDa (NFE2), transcript variant 1

Product Images

Circular map for RC202924

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  Western blot validation of overexpression lysate (Cat# LY427772) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227517 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NFE2 protein (Cat# TP302924). The protein was produced from HEK293T cells transfected with NFE2 cDNA clone (Cat# RC202924) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nuclear Factor Erythroid Derived 2
• Synonyms: NF-E2; p45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202924 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCGTGTCCTCCCCAGCAGAGCAGGAACAGGGTGATACAGCTGTCCACTTCAGAGCTAGGAGAGA
  TGGAACTGACTTGGCAGGAGATCATGTCCATCACCGAGCTGCAGGGTCTGAATGCTCCAAGTGAGCCATC
  ATTTGAGCCCCAAGCCCCAGCTCCATACCTTGGACCTCCACCACCCACAACTTACTGCCCCTGCTCAATC
  CACCCAGATTCTGGCTTCCCACTTCCTCCACCACCTTATGAGCTCCCAGCATCCACATCCCATGTCCCAG
  ATCCCCCATACTCCTATGGCAACATGGCCATACCAGTCTCCAAGCCACTGAGCCTCTCAGGCCTGCTCAG
  TGAGCCGCTCCAAGACCCCTTAGCCCTCCTGGACATTGGGCTGCCAGCAGGGCCACCTAAGCCCCAAGAA
  GACCCAGAATCCGACTCAGGATTATCCCTCAACTATAGCGATGCTGAATCTCTTGAGCTGGAGGGGACAG
  AGGCTGGTCGGCGGCGCAGCGAATATGTAGAGATGTACCCAGTGGAGTACCCCTACTCACTCATGCCCAA
  CTCCTTGGCCCACTCCAACTATACCTTGCCAGCTGCTGAGACCCCCTTGGCCTTAGAGCCCTCCTCAGGC
  CCTGTGCGGGCTAAGCCCACTGCACGGGGGGAGGCAGGGAGTCGGGATGAACGTCGGGCCTTGGCCATGA
  AGATTCCTTTTCCTACGGACAAGATTGTCAACTTGCCGGTAGATGACTTTAATGAGCTATTGGCAAGGTA
  CCCGCTGACAGAGAGCCAGCTAGCGCTAGTCCGGGACATCCGACGACGGGGCAAAAACAAGGTGGCAGCC
  CAGAACTGCCGCAAGAGGAAGCTGGAAACCATTGTGCAGCTGGAGCGGGAGCTGGAGCGGCTGACCAATG
  AACGGGAGCGGCTTCTCAGGGCCCGCGGGGAGGCAGACCGGACCCTGGAGGTCATGCGCCAACAGCTGAC
  AGAGCTGTACCGTGACATTTTCCAGCACCTTCGGGATGAATCAGGCAACAGCTACTCTCCTGAAGAGTAC
  GCGCTGCAACAGGCTGCCGATGGGACCATCTTCCTTGTGCCCCGGGGGACCAAGATGGAGGCCACAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202924 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPCPPQQSRNRVIQLSTSELGEMELTWQEIMSITELQGLNAPSEPSFEPQAPAPYLGPPPPTTYCPCSI
  HPDSGFPLPPPPYELPASTSHVPDPPYSYGNMAIPVSKPLSLSGLLSEPLQDPLALLDIGLPAGPPKPQE
  DPESDSGLSLNYSDAESLELEGTEAGRRRSEYVEMYPVEYPYSLMPNSLAHSNYTLPAAETPLALEPSSG
  PVRAKPTARGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLARYPLTESQLALVRDIRRRGKNKVAA
  QNCRKRKLETIVQLERELERLTNERERLLRARGEADRTLEVMRQQLTELYRDIFQHLRDESGNSYSPEEY
  ALQQAADGTIFLVPRGTKMEATD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006163
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006163.2
• RefSeq Size: 1697 bp
• RefSeq ORF: 1122 bp
• Locus ID: 4778
• UniProt ID: Q16621
• Cytogenetics: 12q13.13
• Protein Families: Transcription Factors
• MW: 41.5 kDa
• Gene Summary: Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.[UniProtKB/Swiss-Prot Function]