Twist (TWIST1) (NM_000474) Human Tagged ORF Clone
TWIST1 (Myc-DDK-tagged)-Human twist homolog 1 (Drosophila) (TWIST1)
|Product Name||Twist (TWIST1) (NM_000474) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ACS3; bHLHa38; BPES2; BPES3; CRS; CRS1; CSO; SCS; SWCOS; TWIST|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC202920 representing NM_000474
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202920 representing NM_000474
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-RsrII Cloning Scheme for this gene Plasmid Map|
|ORF Size||606 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1669 bp|
|RefSeq ORF||609 bp|
|Protein Families||Druggable Genome|
|Gene Summary||This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]|
|The use of this cDNA Clones has been cited in the following citations:|
PLCε regulates prostate cancer mitochondrial oxidative metabolism and migration via upregulation of Twist1
,Fan, J;Fan, Y;Wang, X;Niu, L;Duan, L;Yang, J;Li, L;Gao, Y;Wu, X;Luo, C;,
J. Exp. Clin. Cancer Res. 2019
,PubMed ID 31383001
α-Parvin promotes breast cancer progression and metastasis through interaction with G3BP2 and regulation of TWIST1 signaling
,Sun, Y;Ding, Y;Guo, C;Liu, C;Ma, P;Ma, S;Wang, Z;Liu, J;Qian, T;Ma, L;Deng, Y;Wu, C;,
,PubMed ID 30804457
The FZD7-TWIST1 axis is responsible for anoikis resistance and tumorigenesis in ovarian carcinoma
,Tan, M;Asad, M;Heong, V;Wong, MK;Tan, TZ;Ye, J;Kuay, KT;Thiery, JP;Scott, C;Huang, RY;,
Mol Oncol 2018
,PubMed ID 30548372
TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes
,Hasei, J;Teramura, T;Takehara, T;Onodera, Y;Horii, T;Olmer, M;Hatada, I;Fukuda, K;Ozaki, T;Lotz, MK;Asahara, H;,
Sci Rep 2017
,PubMed ID 28220902
The Role of TWIST in Angiogenesis and Cell Migration in Giant Cell Tumor of Bone
,Singh, S;Mak, IWY;Handa, D;Ghert, M;,
Advances in Biology May 2014
|cDNA Clone Resources|
|SC321467||TWIST1 (untagged)-Human twist homolog 1 (Drosophila) (TWIST1)||
|RG202920||TWIST1 (GFP-tagged) - Human twist homolog 1 (Drosophila) (TWIST1)||
|RC202920L1||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged||
|RC202920L2||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged||
|RC202920L3||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), Myc-DDK-tagged||
|RC202920L4||Lenti ORF clone of Human twist homolog 1 (Drosophila) (TWIST1), mGFP tagged||