DHX16 (NM_003587) Human Tagged ORF Clone

CAT#: RC202912

DHX16 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 16 (DHX16), transcript variant 1

Product Images

Circular map for RC202912

• 

  Western blot validation of overexpression lysate (Cat# LY418557) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202912 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DHX16 protein (Cat# TP302912). The protein was produced from HEK293T cells transfected with DHX16 cDNA clone (Cat# RC202912) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DHX16
• Synonyms: DBP2; DDX16; NMOAS; PRO2014; Prp2; PRP8; PRPF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202912 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGCCGGCGGGTCTGGAGCGCTGGGTTCAGGACGAGCTGCACTCGGTGTTGGGGCTGAGCGAGC
  GGCACGTCGCCCAGTTTCTGATCGGTACCGCACAGCGCTGCACCTCTGCCGAGGAGTTCGTGCAGCGCCT
  ACGAGACACTGATACCTTGGATCTCAGTGGGCCGGCCCGGGACTTCGCCCTGAGACTCTGGAACAAGGTA
  CCACGAAAGGCAGTGGTAGAAAAGCCAGCTCGGGCAGCAGAGCGAGAGGCCCGGGCCCTGCTGGAGAAGA
  ACCGATCTTATAGGTTACTGGAAGACAGTGAAGAGAGCAGTGAGGAGACTGTGAGTAGGGCTGGAAGCAG
  CCTCCAGAAGAAACGTAAAAAGCGGAAACACCTCAGGAAGAAGCGTGAGGAAGAAGAGGAGGAAGAGGCT
  TCTGAGAAAGGGAAGAAGAAAACAGGGGGGAGTAAACAGCAGACAGAGAAGCCAGAGTCGGAAGATGAGT
  GGGAACGGACAGAGCGTGAACGCCTTCAGGACCTGGAGGAGCGTGATGCCTTTGCTGAGCGGGTTCGACA
  GCGGGACAAGGATCGGACTCGAAATGTCCTGGAACGGTCAGACAAGAAGGCTTATGAAGAGGCTCAGAAG
  CGCCTCAAGATGGCCGAGGAAGACCGGAAGGCCATGGTCCCTGAGCTGCGGAAGAAATCTCGCCGAGAGT
  ACCTGGCTAAGCGGGAGCGAGAGAAGCTTGAGGACCTGGAGGCGGAGCTGGCTGATGAGGAGTTCCTTTT
  TGGGGACGTGGAGCTGAGCCGGCACGAGCGGCAGGAGCTCAAATATAAGCGGCGAGTGCGGGATCTCGCC
  CGGGAGTACCGGGCAGCTGGGGAGCAGGAGAAGCTGGAGGCCACCAATCGCTACCACATGCCCAAGGAAA
  CCCGAGGACAGCCAGCCCGAGCTGTGGATCTAGTGGAGGAGGAATCAGGAGCCCCTGGGGAGGAGCAGCG
  GCGCTGGGAGGAGGCGCGGCTTGGGGCAGCGTCCCTGAAGTTTGGGGCCCGAGATGCTGCCTCTCAGGAG
  CCCAAGTATCAACTGGTGCTGGAGGAGGAGGAGACCATTGAGTTTGTCCGGGCCACTCAGCTCCAGGGTG
  ATGAGGAGCCGTCAGCTCCACCCACTTCAACTCAGGCCCAGCAGAAAGAGTCCATCCAGGCCGTCCGCCG
  CAGCCTCCCGGTGTTCCCATTTCGAGAGGAGCTCCTGGCTGCTATTGCAAATCACCAAGTCCTCATCATT
  GAAGGCGAGACAGGCTCAGGGAAGACCACCCAGATCCCGCAGTATCTCTTTGAGGAGGGTTATACAAACA
  AGGGTATGAAGATTGCCTGCACCCAACCCCGGAGAGTGGCTGCCATGAGTGTGGCCGCCCGAGTGGCCCG
  GGAGATGGGTGTGAAGCTTGGGAATGAGGTTGGCTACAGCATCCGCTTTGAGGACTGCACATCAGAGCGA
  ACTGTCCTCCGCTACATGACAGATGGGATGCTTCTCCGGGAGTTCCTCTCTGAGCCTGACCTGGCGAGTT
  ACAGCGTGGTGATGGTGGATGAGGCACACGAAAGGACCCTACACACAGACATTCTCTTTGGATTGATCAA
  GGATGTTGCTCGCTTCCGACCTGAGCTCAAGGTCCTGGTGGCTTCAGCCACAATGGACACTGCCCGTTTT
  TCCACCTTCTTTGATGACGCCCCTGTGTTTCGAATCCCCGGACGCAGGTTTCCTGTGGACATCTTCTACA
  CCAAGGCTCCAGAGGCTGACTACTTGGAAGCTTGTGTAGTATCTGTGTTGCAGATCCATGTGACCCAGCC
  CCCTGGGGATATCCTGGTGTTCCTGACAGGACAGGAGGAGATTGAGGCTGCCTGTGAGATGCTCCAGGAT
  CGCTGCCGCCGCCTGGGCTCCAAAATCCGGGAGCTCCTGGTGCTGCCCATTTATGCCAATCTGCCCTCTG
  ACATGCAGGCCCGTATCTTCCAGCCCACACCACCTGGGGCACGAAAGGTGGTTGTGGCAACGAACATTGC
  TGAGACATCACTCACCATTGAGGGCATCATTTATGTGCTGGATCCAGGGTTCTGTAAGCAGAAGAGCTAC
  AACCCCCGCACAGGCATGGAATCGCTCACTGTCACACCCTGCAGCAAGGCCTCAGCCAATCAGCGAGCTG
  GCAGGGCAGGTCGGGTGGCTGCAGGGAAGTGCTTCCGCCTGTATACCGCCTGGGCCTATCAGCACGAGCT
  TGAGGAAACCACAGTGCCTGAGATCCAGAGGACCAGCTTGGGCAATGTCGTGTTGCTGCTCAAGAGCTTA
  GGGATCCATGACCTAATGCACTTTGATTTCCTGGACCCTCCACCATATGAGACACTGCTGCTGGCTTTGG
  AGCAGCTGTATGCTCTGGGAGCCCTCAACCACCTTGGGGAGCTCACCACGTCTGGTCGAAAGATGGCAGA
  GCTGCCGGTGGACCCCATGCTGTCCAAAATGATCTTAGCCTCTGAGAAGTACAGCTGTTCAGAGGAGATC
  CTGACAGTGGCTGCCATGCTCTCTGTCAACAACTCCATCTTCTACCGACCAAAGGACAAGGTCGTCCATG
  CTGACAATGCCCGTGTCAACTTCTTTCTCCCTGGCGGTGACCACCTGGTTCTGCTAAATGTTTACACACA
  GTGGGCTGAGAGTGGTTACTCTTCCCAGTGGTGCTATGAGAACTTTGTACAGTTCAGATCGATGCGCCGA
  GCCCGGGATGTGCGGGAACAGCTGGAAGGGCTCTTGGAACGTGTGGAAGTTGGTCTCAGTTCCTGCCAGG
  GGGACTATATCCGTGTACGCAAGGCCATCACTGCTGGTTACTTTTACCACACGGCACGGTTGACTCGGAG
  TGGCTACCGCACAGTGAAACAGCAGCAGACAGTCTTCATTCATCCCAACTCCTCCCTCTTTGAGCAACAG
  CCACGCTGGCTGCTCTACCACGAACTTGTCTTGACCACCAAAGAGTTCATGAGACAGGTACTGGAGATTG
  AGAGCAGTTGGCTTCTGGAGGTGGCTCCCCATTATTATAAGGCCAAGGAGCTAGAAGATCCCCATGCTAA
  GAAAATGCCCAAAAAAAAAATAGGCAAAACACGAGAAGAGCTAGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202912 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPAGLERWVQDELHSVLGLSERHVAQFLIGTAQRCTSAEEFVQRLRDTDTLDLSGPARDFALRLWNKV
  PRKAVVEKPARAAEREARALLEKNRSYRLLEDSEESSEETVSRAGSSLQKKRKKRKHLRKKREEEEEEEA
  SEKGKKKTGGSKQQTEKPESEDEWERTERERLQDLEERDAFAERVRQRDKDRTRNVLERSDKKAYEEAQK
  RLKMAEEDRKAMVPELRKKSRREYLAKREREKLEDLEAELADEEFLFGDVELSRHERQELKYKRRVRDLA
  REYRAAGEQEKLEATNRYHMPKETRGQPARAVDLVEEESGAPGEEQRRWEEARLGAASLKFGARDAASQE
  PKYQLVLEEEETIEFVRATQLQGDEEPSAPPTSTQAQQKESIQAVRRSLPVFPFREELLAAIANHQVLII
  EGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSER
  TVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARF
  STFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHVTQPPGDILVFLTGQEEIEAACEMLQD
  RCRRLGSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGIIYVLDPGFCKQKSY
  NPRTGMESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSL
  GIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEI
  LTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRR
  ARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSGYRTVKQQQTVFIHPNSSLFEQQ
  PRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKELEDPHAKKMPKKKIGKTREELG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003587
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003587.5
• RefSeq Size: 3477 bp
• RefSeq ORF: 3126 bp
• Locus ID: 8449
• UniProt ID: O60231
• Cytogenetics: 6p21.33
• Domains: DEAD, helicase_C, HA2
• Protein Pathways: Spliceosome
• MW: 119.4 kDa
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. This gene is mapped to the MHC region on chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2018]