SERINC3 (NM_198941) Human Tagged ORF Clone

CAT#: RC202866

SERINC3 (Myc-DDK-tagged)-Human serine incorporator 3 (SERINC3), transcript variant 2

Product Images

Circular map for RC202866

• 

  Western blot validation of overexpression lysate (Cat# LY404656) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202866 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SERINC3
• Synonyms: AIGP1; DIFF33; SBBI99; TDE; TDE1; TMS-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202866 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCTGTGCTGGGTGTCTTCTCCCTCGCCAGCTGGGTTCCATGCCTCTGCAGCGGTGCCTCATGTT
  TGCTGTGTAGTTGCTGTCCTAACAGTAAGAATTCCACGGTGACTCGCCTCATTTATGCTTTCATTCTCCT
  CCTGAGCACTGTCGTATCCTATATCATGCAGAGAAAAGAGATGGAAACTTACTTGAAGAAGATTCCTGGA
  TTTTGTGAAGGGGGATTTAAAATCCATGAGGCTGATATAAATGCAGATAAAGATTGTGATGTGCTGGTTG
  GTTATAAAGCTGTGTATCGGATCAGCTTTGCCATGGCCATCTTTTTCTTTGTCTTTTCTCTGCTCATGTT
  CAAAGTAAAAACAAGTAAAGATCTCCGAGCGGCAGTACACAATGGGTTTTGGTTCTTCAAAATTGCTGCC
  CTTATTGGAATCATGGTTGGCTCTTTCTACATCCCTGGGGGCTATTTCAGCTCAGTCTGGTTTGTTGTTG
  GCATGATAGGGGCCGCCCTCTTCATCCTCATTCAGCTGGTGCTGCTGGTAGATTTTGCTCATTCTTGGAA
  TGAATCATGGGTAAATCGAATGGAAGAAGGAAACCCAAGGTTGTGGTATGCTGCTTTACTGTCTTTCACA
  AGCGCCTTTTATATCCTGTCAATCATCTGTGTCGGGCTGCTCTATACATATTACACCAAACCAGATGGCT
  GCACAGAAAACAAGTTCTTCATCAGTATTAACCTGATCCTTTGCGTTGTGGCTTCTATTATATCGATCCA
  CCCAAAAATTCAGGAACACCAGCCTCGCTCCGGCCTCTTGCAGTCCTCCCTCATCACCCTCTACACTATG
  TACCTCACCTGGTCAGCCATGTCCAATGAACCTGATCGTTCCTGCAATCCCAACCTGATGAGCTTTATTA
  CACGCATAACTGCACCAACCCTGGCTCCTGGAAATTCAACTGCTGTGGTCCCTACCCCTACTCCACCATC
  AAAGAGTGGGTCTTTACTGGATTCAGATAATTTTATTGGACTGTTTGTCTTTGTTCTCTGCCTCTTGTAT
  TCTAGCATCCGCACTTCCACTAATAGCCAAGTAGACAAGCTGACCCTGTCAGGGAGTGACAGCGTCATCC
  TTGGTGATACAACTACCAGTGGTGCCAGTGATGAAGAAGATGGACAGCCTCGGCGGGCTGTGGACAACGA
  GAAAGAGGGAGTGCAGTATAGCTACTCCTTATTCCACCTCATGCTCTGCTTGGCTTCCTTGTACATCATG
  ATGACCCTGACCAGCTGGTACAGCCCTGATGCAAAGTTTCAGAGCATGACCAGCAAGTGGCCAGCTGTGT
  GGGTCAAGATCAGCTCCAGCTGGGTCTGCCTCCTGCTTTACGTCTGGACCCTTGTGGCTCCACTTGTCCT
  CACCAGTCGGGACTTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202866 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAVLGVFSLASWVPCLCSGASCLLCSCCPNSKNSTVTRLIYAFILLLSTVVSYIMQRKEMETYLKKIPG
  FCEGGFKIHEADINADKDCDVLVGYKAVYRISFAMAIFFFVFSLLMFKVKTSKDLRAAVHNGFWFFKIAA
  LIGIMVGSFYIPGGYFSSVWFVVGMIGAALFILIQLVLLVDFAHSWNESWVNRMEEGNPRLWYAALLSFT
  SAFYILSIICVGLLYTYYTKPDGCTENKFFISINLILCVVASIISIHPKIQEHQPRSGLLQSSLITLYTM
  YLTWSAMSNEPDRSCNPNLMSFITRITAPTLAPGNSTAVVPTPTPPSKSGSLLDSDNFIGLFVFVLCLLY
  SSIRTSTNSQVDKLTLSGSDSVILGDTTTSGASDEEDGQPRRAVDNEKEGVQYSYSLFHLMLCLASLYIM
  MTLTSWYSPDAKFQSMTSKWPAVWVKISSSWVCLLLYVWTLVAPLVLTSRDFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198941
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198941.2
• RefSeq Size: 1744 bp
• RefSeq ORF: 1422 bp
• Locus ID: 10955
• UniProt ID: Q13530
• Cytogenetics: 20q13.12
• Protein Families: Transmembrane
• MW: 52.6 kDa
• Gene Summary: Restriction factor required to restrict infectivity of lentiviruses, such as HIV-1: acts by inhibiting an early step of viral infection. Impairs the penetration of the viral particle into the cytoplasm (PubMed:26416733, PubMed:26416734).[UniProtKB/Swiss-Prot Function]