PSMC6 (NM_002806) Human Tagged ORF Clone

CAT#: RC202809

PSMC6 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 6 (PSMC6)

Product Images

Circular map for RC202809

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  Western blot validation of overexpression lysate (Cat# LY400994) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202809 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PSMC6 protein (Cat# TP302809). The protein was produced from HEK293T cells transfected with PSMC6 cDNA clone (Cat# RC202809) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMC6
• Synonyms: p42; RPT5; SUG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202809 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCCTAGAGATAAGGCGCTTCAGGACTACCGCAAGAAGTTGCTTGAACACAAGGAGATCGACG
  GCCGTCTTAAGGAGTTAAGGGAACAATTAAAAGAACTTACCAAGCAGTATGAAAAGTCTGAAAATGATCT
  GAAGGCCCTACAGAGTGTTGGGCAGATCGTGGGTGAAGTGCTTAAACAGTTAACTGAAGAAAAATTCATT
  GTTAAAGCTACCAATGGACCAAGATATGTTGTGGGTTGTCGTCGACAGCTTGACAAAAGTAAGCTGAAGC
  CAGGAACAAGAGTTGCTTTGGATATGACTACACTAACTATCATGAGATATTTGCCGAGAGAGGTGGATCC
  ACTGGTTTATAACATGTCTCATGAGGACCCTGGGAATGTTTCTTATTCTGAGATTGGAGGGCTATCAGAA
  CAGATCCGGGAATTAAGAGAGGTGATAGAATTACCTCTTACAAACCCAGAGTTATTTCAGCGTGTAGGAA
  TAATACCTCCAAAAGGCTGTTTGTTATATGGACCACCAGGTACGGGAAAAACACTCTTGGCACGAGCCGT
  TGCTAGCCAGCTGGACTGCAATTTCTTAAAGGTTGTATCTAGTTCTATTGTAGACAAGTACATTGGTGAA
  AGTGCTCGTTTGATCAGAGAAATGTTTAATTATGCTAGAGATCATCAACCATGCATCATTTTTATGGATG
  AAATAGATGCTATTGGTGGTCGTCGGTTTTCTGAGGGTACTTCAGCTGACAGAGAGATTCAGAGAACGTT
  AATGGAGTTACTGAATCAAATGGATGGATTTGATACTCTGCATAGAGTTAAAATGATCATGGCTACAAAC
  AGACCAGATACACTGGATCCTGCTTTGCTGCGTCCAGGAAGATTAGATAGAAAAATACATATTGATTTGC
  CAAATGAACAAGCAAGATTAGACATACTGAAAATCCATGCAGGTCCCATTACAAAGCATGGTGAAATAGA
  TTATGAAGCAATTGTGAAGCTTTCGGATGGCTTTAATGGAGCAGATCTGAGAAATGTTTGTACTGAAGCA
  GGTATGTTCGCAATTCGTGCTGATCATGATTTTGTAGTACAGGAAGACTTCATGAAAGCAGTCAGAAAAG
  TGGCTGATTCTAAGAAGCTGGAGTCTAAATTGGACTACAAACCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202809 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADPRDKALQDYRKKLLEHKEIDGRLKELREQLKELTKQYEKSENDLKALQSVGQIVGEVLKQLTEEKFI
  VKATNGPRYVVGCRRQLDKSKLKPGTRVALDMTTLTIMRYLPREVDPLVYNMSHEDPGNVSYSEIGGLSE
  QIRELREVIELPLTNPELFQRVGIIPPKGCLLYGPPGTGKTLLARAVASQLDCNFLKVVSSSIVDKYIGE
  SARLIREMFNYARDHQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLHRVKMIMATN
  RPDTLDPALLRPGRLDRKIHIDLPNEQARLDILKIHAGPITKHGEIDYEAIVKLSDGFNGADLRNVCTEA
  GMFAIRADHDFVVQEDFMKAVRKVADSKKLESKLDYKPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002806
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002806.5
• RefSeq Size: 1599 bp
• RefSeq ORF: 1170 bp
• Locus ID: 5706
• UniProt ID: P62333
• Cytogenetics: 14q22.1
• Domains: AAA, AAA
• Protein Pathways: Proteasome
• MW: 44.2 kDa
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008]