PSMA4 (NM_002789) Human Tagged ORF Clone

CAT#: RC202786

PSMA4 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, alpha type, 4 (PSMA4), transcript variant 1

Product Images

Circular map for RC202786

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PSMA4 (Cat# RC202786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4(Cat# TA504059). Positive lysates LY419117 (100ug) and LC419117 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY420186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PSMA4 protein (Cat# TP302786). The protein was produced from HEK293T cells transfected with PSMA4 cDNA clone (Cat# RC202786) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMA4
• Synonyms: HC9; HsT17706; PSC9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202786 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGAAGATATGACTCCAGGACCACTATATTTTCTCCAGAAGGTCGCTTATACCAAGTTGAATATG
  CCATGGAAGCTATTGGACATGCAGGCACCTGTTTGGGAATTTTAGCAAATGATGGTGTTTTGCTTGCAGC
  AGAGAGACGCAACATCCACAAGCTTCTTGATGAAGTCTTTTTTTCTGAAAAAATTTATAAACTCAATGAG
  GACATGGCTTGCAGTGTGGCAGGCATAACTTCTGATGCTAATGTTCTGACTAATGAACTAAGGCTCATTG
  CTCAAAGGTATTTATTACAGTATCAGGAGCCAATACCTTGTGAGCAGTTGGTTACAGCGCTGTGTGATAT
  CAAACAAGCTTATACACAATTTGGAGGAAAACGTCCCTTTGGTGTTTCATTGCTGTACATTGGCTGGGAT
  AAGCACTATGGCTTTCAGCTCTATCAGAGTGACCCTAGTGGAAATTACGGGGGATGGAAGGCCACATGCA
  TTGGAAATAATAGCGCTGCAGCTGTGTCAATGTTGAAACAAGACTATAAAGAAGGAGAAATGACCTTGAA
  GTCAGCACTTGCTTTAGCTATCAAAGTACTAAATAAGACCATGGATGTTAGTAAACTCTCTGCTGAAAAA
  GTGGAAATTGCAACACTAACAAGAGAGAATGGAAAGACAGTAATCAGAGTTCTCAAACAAAAAGAAGTGG
  AGCAGTTGATCAAAAAACACGAGGAAGAAGAAGCCAAAGCTGAGCGTGAGAAGAAAGAAAAAGAACAGAA
  AGAAAAGGATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202786 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNE
  DMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWD
  KHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEK
  VEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002789
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002789.2
• RefSeq Size: 1235 bp
• RefSeq ORF: 786 bp
• Locus ID: 5685
• UniProt ID: P25789
• Cytogenetics: 15q25.1
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• MW: 29.5 kDa
• Gene Summary: This gene encodes a core alpha subunit of the 20S proteosome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. Proteasomes cleave peptides in an ATP- and ubiquitin-dependent manner. [provided by RefSeq, Aug 2016]