LXN (NM_020169) Human Tagged ORF Clone

CAT#: RC202769

LXN (Myc-DDK-tagged)-Human latexin (LXN)

Product Images

Circular map for RC202769

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LXN (Cat# RC202769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN(Cat# TA503779). Positive lysates LY412596 (100ug) and LC412596 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202769 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LXN protein (Cat# TP302769). The protein was produced from HEK293T cells transfected with LXN cDNA clone (Cat# RC202769) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LXN
• Synonyms: ECI; TCI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202769 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCCCGCCGACCAACTACCCAGCCTCCAGGGCGGCCTTGGTGGCACAGAACTACATCAACTACC
  AGCAGGGGACCCCGCACAGGGTGTTTGAGGTGCAGAAGGTCAAACAAGCCAGCATGGAGGATATTCCAGG
  AAGAGGACATAAGTATCGCCTTAAATTTGCTGTTGAAGAAATTATACAAAAACAAGTTAAGGTGAACTGC
  ACAGCTGAAGTACTTTACCCTTCAACGGGACAAGAAACTGCACCAGAAGTCAACTTCACATTTGAAGGAG
  AAACTGGAAAGAATCCAGATGAAGAAGACAACACATTTTATCAAAGACTTAAGTCCATGAAGGAACCGCT
  AGAAGCACAAAATATTCCAGACAATTTTGGAAATGTATCTCCAGAAATGACGCTCGTTCTACATTTAGCC
  TGGGTTGCCTGTGGTTATATAATATGGCAAAATTCTACTGAAGACACATGGTATAAAATGGTAAAAATTC
  AAACTGTCAAGCAAGTGCAAAGAAATGATGACTTTATTGAATTAGACTACACCATTCTACTTCATAATAT
  AGCATCTCAGGAGATTATTCCCTGGCAAATGCAAGTTCTCTGGCATCCACAATACGGCACTAAAGTAAAA
  CATAATAGCCGTCTGCCAAAGGAAGTACAACTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202769 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEIPPTNYPASRAALVAQNYINYQQGTPHRVFEVQKVKQASMEDIPGRGHKYRLKFAVEEIIQKQVKVNC
  TAEVLYPSTGQETAPEVNFTFEGETGKNPDEEDNTFYQRLKSMKEPLEAQNIPDNFGNVSPEMTLVLHLA
  WVACGYIIWQNSTEDTWYKMVKIQTVKQVQRNDDFIELDYTILLHNIASQEIIPWQMQVLWHPQYGTKVK
  HNSRLPKEVQLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020169
• ORF Size: 666 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020169.2, NP_064554.2
• RefSeq Size: 1132 bp
• RefSeq ORF: 669 bp
• Locus ID: 56925
• UniProt ID: Q9BS40
• Cytogenetics: 3q25.32
• MW: 25.8 kDa
• Gene Summary: This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. The encoded protein, latexin, downregulates the population size of hematopoietic stem cells. This protein is found to be downregulated in cancer cells because of promoter hypermethylation. [provided by RefSeq, Jul 2020]