LXN (NM_020169) Human Tagged ORF Clone

CAT#: RC202769

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LXN (Myc-DDK-tagged)-Human latexin (LXN)



  "NM_020169" in other vectors (7)

Reconstitution Protocol

USD 300.00

USD 450.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LXN
Synonyms ECI; TCI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202769 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATCCCGCCGACCAACTACCCAGCCTCCAGGGCGGCCTTGGTGGCACAGAACTACATCAACTACC
AGCAGGGGACCCCGCACAGGGTGTTTGAGGTGCAGAAGGTCAAACAAGCCAGCATGGAGGATATTCCAGG
AAGAGGACATAAGTATCGCCTTAAATTTGCTGTTGAAGAAATTATACAAAAACAAGTTAAGGTGAACTGC
ACAGCTGAAGTACTTTACCCTTCAACGGGACAAGAAACTGCACCAGAAGTCAACTTCACATTTGAAGGAG
AAACTGGAAAGAATCCAGATGAAGAAGACAACACATTTTATCAAAGACTTAAGTCCATGAAGGAACCGCT
AGAAGCACAAAATATTCCAGACAATTTTGGAAATGTATCTCCAGAAATGACGCTCGTTCTACATTTAGCC
TGGGTTGCCTGTGGTTATATAATATGGCAAAATTCTACTGAAGACACATGGTATAAAATGGTAAAAATTC
AAACTGTCAAGCAAGTGCAAAGAAATGATGACTTTATTGAATTAGACTACACCATTCTACTTCATAATAT
AGCATCTCAGGAGATTATTCCCTGGCAAATGCAAGTTCTCTGGCATCCACAATACGGCACTAAAGTAAAA
CATAATAGCCGTCTGCCAAAGGAAGTACAACTGGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202769 protein sequence
Red=Cloning site Green=Tags(s)

MEIPPTNYPASRAALVAQNYINYQQGTPHRVFEVQKVKQASMEDIPGRGHKYRLKFAVEEIIQKQVKVNC
TAEVLYPSTGQETAPEVNFTFEGETGKNPDEEDNTFYQRLKSMKEPLEAQNIPDNFGNVSPEMTLVLHLA
WVACGYIIWQNSTEDTWYKMVKIQTVKQVQRNDDFIELDYTILLHNIASQEIIPWQMQVLWHPQYGTKVK
HNSRLPKEVQLE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020169
ORF Size 666 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020169.2, NP_064554.2
RefSeq Size 1132 bp
RefSeq ORF 669 bp
Locus ID 56925
UniProt ID Q9BS40
Cytogenetics 3q25.32
MW 25.8 kDa
Gene Summary This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases. The encoded protein, latexin, downregulates the population size of hematopoietic stem cells. This protein is found to be downregulated in cancer cells because of promoter hypermethylation. [provided by RefSeq, Jul 2020]

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