Creatine kinase M type (CKM) (NM_001824) Human Tagged ORF Clone

CAT#: RC202721

CKM (Myc-DDK-tagged)-Human creatine kinase, muscle (CKM)

Product Images

Circular map for RC202721

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  Western blot validation of overexpression lysate (Cat# LY400690) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202721 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CKM protein (Cat# TP302721). The protein was produced from HEK293T cells transfected with CKM cDNA clone (Cat# RC202721) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Creatine kinase M type
• Synonyms: CKMM; CPK-M; M-CK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202721 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATTCGGTAACACCCACAACAAGTTCAAGCTGAATTACAAGCCTGAGGAGGAGTACCCCGACCTCA
  GCAAACATAACAACCACATGGCCAAGGTACTGACCCTTGAACTCTACAAGAAGCTGCGGGACAAGGAGAC
  TCCATCTGGCTTCACTGTAGACGATGTCATCCAGACAGGAGTGGACAACCCAGGTCACCCCTTCATCATG
  ACCGTGGGCTGCGTGGCTGGTGATGAGGAGTCCTACGAAGTTTTCAAGGAACTCTTTGACCCCATCATCT
  CGGATCGCCACGGGGGCTACAAACCCACTGACAAGCACAAGACTGACCTCAACCATGAAAACCTCAAGGG
  TGGAGACGACCTGGACCCCAACTACGTGCTCAGCAGCCGCGTCCGCACTGGCCGCAGCATCAAGGGCTAC
  ACGTTGCCCCCACACTGCTCCCGTGGCGAGCGCCGGGCGGTGGAGAAGCTCTCTGTGGAAGCTCTCAACA
  GCCTGACGGGCGAGTTCAAAGGGAAGTACTACCCTCTGAAGAGCATGACGGAGAAGGAGCAGCAGCAGCT
  CATCGATGACCACTTCCTGTTCGACAAGCCCGTGTCCCCGCTGCTGCTGGCCTCAGGCATGGCCCGCGAC
  TGGCCCGACGCCCGTGGCATCTGGCACAATGACAACAAGAGCCTCCTGGTGTGGGTGAACGAGGAGGATC
  ACCTCCGGGTCATCTCCATGGAGAAGGGGGGCAACATGAAGGAGGTTTTCCGCCGCTTCTGCGTAGGGCT
  GCAGAAGATTGAGGAGATCTTTAAGAAAGCTGGCCACCCCTTCATGTGGAACCAGCACCTGGGCTACGTG
  CTCACCTGCCCATCCAACCTGGGCACTGGGCTGCGTGGAGGCGTGCATGTGAAGCTGGCGCACCTGAGCA
  AGCACCCCAAGTTCGAGGAGATCCTCACCCGCCTGCGTCTGCAGAAGAGGGGTACAGGTGGCGTGGACAC
  AGCTGCCGTGGGCTCAGTATTTGACGTGTCCAACGCTGATCGGCTGGGCTCGTCCGAAGTAGAACAGGTG
  CAGCTGGTGGTGGATGGTGTGAAGCTCATGGTGGAAATGGAGAAGAAGTTGGAGAAAGGCCAGTCCATCG
  ACGACATGATCCCCGCCCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202721 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIM
  TVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGY
  TLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARD
  WPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYV
  LTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQV
  QLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001824
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001824.5
• RefSeq Size: 1666 bp
• RefSeq ORF: 1146 bp
• Locus ID: 1158
• UniProt ID: P06732
• Cytogenetics: 19q13.32
• Domains: ATP-gua_Ptrans
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 43.1 kDa
• Gene Summary: The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]