PTEN (NM_000314) Human Tagged ORF Clone

CAT#: RC202627L4

  • LentiORF®

Lenti ORF clone of Human phosphatase and tensin homolog (PTEN), mGFP tagged

  View "NM_000314" in other vectors (53)

USD 860.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name PTEN (NM_000314) Human Tagged ORF Clone
Symbol PTEN
Synonyms 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
Vector pLenti-C-mGFP-P2A-Puro
Sequence Data
The ORF insert of this clone is exactly the same as(RC202627).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
Tag mGFP
ACCN NM_000314
ORF Size 1209 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_000314.3, NP_000305
RefSeq Size 3416
RefSeq ORF 1212
Locus ID 5728
Cytogenetics 10q23.31
Domains PTPc_motif
Protein Families Phosphatase, Druggable Genome
Protein Pathways Inositol phosphate metabolism, Phosphatidylinositol signaling system, p53 signaling pathway, Focal adhesion, Tight junction, Pathways in cancer, Endometrial cancer, Glioma, Prostate cancer, Melanoma, Small cell lung cancer
MW 47 kDa
Gene Summary This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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