URP2 (FERMT3) (NM_031471) Human Tagged ORF Clone

CAT#: RC202580

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FERMT3 (Myc-DDK-tagged)-Human fermitin family member 3 (FERMT3), transcript variant URP2SF



  "NM_031471" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FERMT3
Synonyms KIND3; MIG-2; MIG2B; UNC112C; URP2; URP2SF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202580 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGATGAAGACAGCCTCCGGGGACTACATCGACTCGTCATGGGAGCTGCGGGTGTTTGTGGGAG
AGGAGGACCCAGAGGCCGAGTCGGTCACCCTGCGGGTCACTGGGGAGTCGCACATCGGCGGGGTGCTCCT
GAAGATTGTGGAGCAGATCAATCGCAAGCAGGACTGGTCAGACCATGCTATTTGGTGGGAACAGAAGAGG
CAGTGGCTGCTGCAGACCCACTGGACACTGGACAAGTACGGGATCCTGGCCGACGCACGCCTCTTCTTTG
GGCCCCAGCACCGGCCCGTCATCCTTCGGTTGCCCAACCGCCGCGCACTGCGCCTCCGTGCCAGCTTCTC
CCAGCCCCTCTTCCAGGCTGTGGCTGCCATCTGCCGCCTCCTCAGCATCCGGCACCCCGAGGAGCTGTCC
CTGCTCCGGGCTCCTGAGAAGAAGGAGAAGAAGAAGAAAGAGAAGGAGCCAGAGGAAGAGCTCTATGACT
TGAGCAAGGTTGTCTTGGCTGGGGGCGTGGCACCTGCACTGTTCCGGGGGATGCCAGCTCACTTCTCGGA
CAGCGCCCAGACTGAGGCCTGCTACCACATGCTGAGCCGGCCCCAGCCGCCACCCGACCCCCTCCTGCTC
CAGCGTCTGCCACGGCCCAGCTCCCTGTCAGACAAGACCCAGCTCCACAGCAGGTGGCTGGACTCGTCGC
GGTGTCTCATGCAGCAGGGCATCAAGGCTGGGGACGCACTCTGGCTGCGCTTCAAGTACTACAGCTTCTT
CGATTTGGATCCCAAGACAGACCCCGTGCGGCTGACACAGCTGTATGAGCAGGCCCGGTGGGACCTGCTG
CTGGAGGAGATTGACTGCACCGAGGAGGAGATGATGGTGTTTGCCGCCCTGCAGTACCACATCAACAAGC
TGTCCCAGAGCGGGGAGGTGGGGGAGCCGGCTGGCACAGACCCAGGGCTGGACGACCTGGATGTGGCCCT
GAGCAACCTGGAGGTGAAGCTGGAGGGGTCGGCGCCCACAGATGTGCTGGACAGCCTCACCACCATCCCA
GAGCTCAAGGACCATCTCCGAATCTTTCGGCCCCGGAAGCTGACCCTGAAGGGCTACCGCCAACACTGGG
TGGTGTTCAAGGAGACCACACTGTCCTACTACAAGAGCCAGGACGAGGCCCCTGGGGACCCCATTCAGCA
GCTCAACCTCAAGGGCTGTGAGGTGGTTCCCGATGTTAACGTCTCCGGCCAGAAGTTCTGCATTAAACTC
CTAGTGCCCTCCCCTGAGGGCATGAGTGAGATCTACCTGCGGTGCCAGGATGAGCAGCAGTATGCCCGCT
GGATGGCTGGCTGCCGCCTGGCCTCCAAAGGCCGCACCATGGCCGACAGCAGCTACACCAGCGAGGTGCA
GGCCATCCTGGCCTTCCTCAGCCTGCAGCGCACGGGCAGTGGGGGCCCGGGCAACCACCCCCACGGCCCT
GATGCCTCTGCCGAGGGCCTCAACCCCTACGGCCTCGTTGCCCCCCGTTTCCAGCGAAAGTTCAAGGCCA
AGCAGCTCACCCCACGGATCCTGGAAGCCCACCAGAATGTGGCCCAGTTGTCGCTGGCAGAGGCCCAGCT
GCGCTTCATCCAGGCCTGGCAGTCCCTGCCCGACTTCGGCATCTCCTATGTCATGGTCAGGTTCAAGGGC
AGCAGGAAAGACGAGATCCTGGGCATCGCCAACAACCGACTGATCCGCATCGACTTGGCCGTGGGCGACG
TGGTCAAGACCTGGCGTTTCAGCAACATGCGCCAGTGGAATGTCAACTGGGACATCCGGCAGGTGGCCAT
CGAGTTTGATGAACACATCAATGTGGCCTTCAGCTGCGTGTCTGCCAGCTGCCGAATTGTACACGAGTAT
ATCGGGGGCTACATTTTCCTGTCGACGCGGGAGCGGGCCCGTGGGGAGGAGCTGGATGAAGACCTCTTCC
TGCAGCTCACCGGGGGCCATGAGGCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202580 protein sequence
Red=Cloning site Green=Tags(s)

MAGMKTASGDYIDSSWELRVFVGEEDPEAESVTLRVTGESHIGGVLLKIVEQINRKQDWSDHAIWWEQKR
QWLLQTHWTLDKYGILADARLFFGPQHRPVILRLPNRRALRLRASFSQPLFQAVAAICRLLSIRHPEELS
LLRAPEKKEKKKKEKEPEEELYDLSKVVLAGGVAPALFRGMPAHFSDSAQTEACYHMLSRPQPPPDPLLL
QRLPRPSSLSDKTQLHSRWLDSSRCLMQQGIKAGDALWLRFKYYSFFDLDPKTDPVRLTQLYEQARWDLL
LEEIDCTEEEMMVFAALQYHINKLSQSGEVGEPAGTDPGLDDLDVALSNLEVKLEGSAPTDVLDSLTTIP
ELKDHLRIFRPRKLTLKGYRQHWVVFKETTLSYYKSQDEAPGDPIQQLNLKGCEVVPDVNVSGQKFCIKL
LVPSPEGMSEIYLRCQDEQQYARWMAGCRLASKGRTMADSSYTSEVQAILAFLSLQRTGSGGPGNHPHGP
DASAEGLNPYGLVAPRFQRKFKAKQLTPRILEAHQNVAQLSLAEAQLRFIQAWQSLPDFGISYVMVRFKG
SRKDEILGIANNRLIRIDLAVGDVVKTWRFSNMRQWNVNWDIRQVAIEFDEHINVAFSCVSASCRIVHEY
IGGYIFLSTRERARGEELDEDLFLQLTGGHEAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031471
ORF Size 1989 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031471.6
RefSeq Size 2558 bp
RefSeq ORF 1992 bp
Locus ID 83706
UniProt ID Q86UX7
Cytogenetics 11q13.1
Domains B41, PH
MW 75.4 kDa
Gene Summary Kindlins are a small family of proteins that mediate protein-protein interactions involved in integrin activation and thereby have a role in cell adhesion, migration, differentiation, and proliferation. The protein encoded by this gene has a key role in the regulation of hemostasis and thrombosis. This protein may also help maintain the membrane skeleton of erythrocytes. Mutations in this gene cause the autosomal recessive leukocyte adhesion deficiency syndrome-III (LAD-III). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2010]

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