FANCG (NM_004629) Human Tagged ORF Clone

CAT#: RC202443

FANCG (Myc-DDK-tagged)-Human Fanconi anemia, complementation group G (FANCG)

Product Images

Circular map for RC202443

• 

  Western blot validation of overexpression lysate (Cat# LY401466) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202443 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FANCG
• Synonyms: FAG; XRCC9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202443 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGCCAGACCACCTCTGTGGGCTCCAGCTGCCTGGACCTGTGGAGGGAAAAGAATGACCGGCTCG
  TTCGACAGGCCAAGGTGGCTCAGAACTCCGGTCTGACTCTGAGGCGACAGCAGTTGGCTCAGGATGCACT
  GGAAGGGCTCAGAGGGCTCCTCCATAGTCTGCAAGGGCTCCCTGCAGCTGTTCCTGTTCTTCCCTTGGAG
  CTGACTGTCACCTGCAACTTCATTATCCTGAGGGCAAGCTTGGCCCAGGGTTTCACAGAGGATCAGGCCC
  AGGATATCCAGCGGAGCCTAGAGAGAGTGCTGGAGACACAGGAGCAGCAGGGGCCCAGGTTGGAACAGGG
  GCTCAGGGAGCTGTGGGACTCTGTCCTTCGTGCTTCCTGCCTTCTGCCGGAGCTGCTGTCTGCCCTGCAC
  CGCCTGGTTGGCCTGCAGGCTGCCCTCTGGTTGAGTGCTGACCGTCTTGGGGACCTGGCCTTGTTACTAG
  AGACCCTGAATGGCAGCCAGAGTGGAGCCTCTAAGGATCTGCTGTTACTTCTGAAAACTTGGAGTCCCCC
  AGCTGAGGAATTAGATGCTCCATTGACCCTGCAGGATGCCCAGGGATTGAAGGATGTCCTCCTGACAGCA
  TTTGCCTACCGCCAAGGTCTCCAGGAGCTGATCACAGGGAACCCAGACAAGGCACTAAGCAGCCTTCATG
  AAGCGGCCTCAGGCCTGTGTCCACGGCCTGTGTTGGTCCAGGTGTACACAGCACTGGGGTCCTGTCACCG
  TAAGATGGGAAATCCACAGAGAGCACTGTTGTACTTGGTTGCAGCCCTGAAAGAGGGATCAGCCTGGGGT
  CCTCCACTTCTGGAGGCCTCTAGGCTCTATCAGCAACTGGGGGACACAACAGCAGAGCTGGAGAGTCTGG
  AGCTGCTAGTTGAGGCCTTGAATGTCCCATGCAGTTCCAAAGCCCCGCAGTTTCTCATTGAGGTAGAATT
  ACTACTGCCACCACCTGACCTAGCCTCACCCCTTCATTGTGGCACTCAGAGCCAGACCAAGCACATACTA
  GCAAGCAGGTGCCTACAGACGGGGAGGGCAGGAGACGCTGCAGAGCATTACTTGGACCTGCTGGCCCTGT
  TGCTGGATAGCTCGGAGCCAAGGTTCTCCCCACCCCCCTCCCCTCCAGGGCCCTGTATGCCTGAGGTGTT
  TTTGGAGGCAGCGGTAGCACTGATCCAGGCAGGCAGAGCCCAAGATGCCTTGACTCTATGTGAGGAGTTG
  CTCAGCCGCACATCATCTCTGCTACCCAAGATGTCCCGGCTGTGGGAAGATGCCAGAAAAGGAACCAAGG
  AACTGCCATACTGCCCACTCTGGGTCTCTGCCACCCACCTGCTTCAGGGCCAGGCCTGGGTTCAACTGGG
  TGCCCAAAAAGTGGCAATTAGTGAATTTAGCAGGTGCCTCGAGCTGCTCTTCCGGGCCACACCTGAGGAA
  AAAGAACAAGGGGCAGCTTTCAACTGTGAGCAGGGATGTAAGTCAGATGCGGCACTGCAGCAGCTTCGGG
  CAGCCGCCCTAATTAGTCGTGGACTGGAATGGGTAGCCAGCGGCCAGGATACCAAAGCCTTACAGGACTT
  CCTCCTCAGTGTGCAGATGTGCCCAGGTAATCGAGACACTTACTTTCACCTGCTTCAGACTCTGAAGAGG
  CTAGATCGGAGGGATGAGGCCACTGCACTCTGGTGGAGGCTGGAGGCCCAAACTAAGGGGTCACATGAAG
  ATGCTCTGTGGTCTCTCCCCCTGTACCTAGAAAGCTATTTGAGCTGGATCCGTCCCTCTGATCGTGACGC
  CTTCCTTGAAGAATTTCGGACATCTCTGCCAAAGTCTTGTGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202443 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLE
  LTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALH
  RLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTA
  FAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWG
  PPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHIL
  ASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEEL
  LSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEE
  KEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKR
  LDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004629
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004629.2
• RefSeq Size: 2649 bp
• RefSeq ORF: 1869 bp
• Locus ID: 2189
• UniProt ID: O15287
• Cytogenetics: 9p13.3
• Domains: TPR
• Protein Families: Druggable Genome
• MW: 68.6 kDa
• Gene Summary: The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq, Jul 2008]