SKA1 (NM_145060) Human Tagged ORF Clone

CAT#: RC202370

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SKA1 (Myc-DDK-tagged)-Human spindle and kinetochore associated complex subunit 1 (SKA1), transcript variant 2



  "NM_145060" in other vectors (5)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SKA1
Synonyms C18orf24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202370 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGTCAGATCTGGAACAATTATGCTCTCATGTTAATGAAAAGATTGGCAATATTAAGAAAACCT
TATCATTAAGAAACTGTGGCCAGGAACCTACCTTGAAAACTGTATTAAATAAAATAGGAGATGAGATCAT
TGTAATAAATGAACTTCTAAATAAATTGGAATTGGAAATTCAGTATCAAGAACAAACCAACAATTCACTC
AAGGAACTCTGTGAATCTCTTGAAGAAGATTACAAAGACATAGAACATCTTAAAGAAAACGTTCCTTCCC
ATTTGCCTCAAGTAACAGTAACCCAGAGCTGTGTTAAGGGATCAGATCTTGATCCTGAAGAACCAATCAA
AGTTGAAGAACCTGAACCCGTAAAGAAGCCTCCCAAAGAGCAAAGAAGTATTAAGGAAATGCCATTTATA
ACTTGTGATGAGTTCAATGGTGTTCCTTCGTACATGAAATCCCGCTTAACCTATAATCAAATTAATGATG
TTATTAAAGAAATCAACAAGGCAGTAATTAGTAAATATAAAATCCTACATCAGCCAAAAAAGTCTATGAA
TTCTGTGACCAGAAATCTCTATCACAGATTTATTGATGAAGAAACGAAGGATACCAAAGGTCGTTATTTT
ATAGTGGAAGCTGACATAAAGGAGTTCACAACTTTGAAAGCTGACAAGAAGTTTCACGTGTTACTGAATA
TTTTACGACACTGCCGGAGGCTATCAGAGGTCCGAGGGGGAGGACTTACTCGTTATGTTATAACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202370 protein sequence
Red=Cloning site Green=Tags(s)

MASSDLEQLCSHVNEKIGNIKKTLSLRNCGQEPTLKTVLNKIGDEIIVINELLNKLELEIQYQEQTNNSL
KELCESLEEDYKDIEHLKENVPSHLPQVTVTQSCVKGSDLDPEEPIKVEEPEPVKKPPKEQRSIKEMPFI
TCDEFNGVPSYMKSRLTYNQINDVIKEINKAVISKYKILHQPKKSMNSVTRNLYHRFIDEETKDTKGRYF
IVEADIKEFTTLKADKKFHVLLNILRHCRRLSEVRGGGLTRYVIT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145060
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145060.4
RefSeq Size 2893 bp
RefSeq ORF 768 bp
Locus ID 220134
UniProt ID Q96BD8
Cytogenetics 18q21.1
MW 29.5 kDa
Gene Summary Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020). In the complex, it mediates the interaction with microtubules (PubMed:19289083, PubMed:23085020).[UniProtKB/Swiss-Prot Function]

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