SQOR (NM_021199) Human Tagged ORF Clone

CAT#: RC202335

SQRDL (Myc-DDK-tagged)-Human sulfide quinone reductase-like (yeast) (SQRDL), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC202335

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  Western blot validation of overexpression lysate (Cat# LY412038) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202335 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SQOR protein (Cat# TP302335). The protein was produced from HEK293T cells transfected with SQOR cDNA clone (Cat# RC202335) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SQOR
• Synonyms: CGI-44; PRO1975; SQR; SQRDL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202335 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCACTGGTGGCTGTGGTATCAGGGCCCCGTGCCCAGCTCTTTGCCTGCCTGCTCAGGCTGGGCA
  CTCAGCAGGTCGGCCCCCTTCAGCTGCACACCGGGGCCAGCCATGCGGCCAGGAACCATTATGAGGTGCT
  GGTGCTGGGTGGGGGCAGTGGCGGAATCACCATGGCTGCCCGCATGAAGAGGAAAGTGGGTGCAGAGAAT
  GTGGCCATTGTTGAGCCCAGTGAGAGACATTTCTACCAGCCAATCTGGACACTGGTGGGTGCTGGTGCCA
  AACAATTGTCCTCATCTGGTCGTCCCACGGCAAGTGTGATTCCATCTGGTGTAGAATGGATCAAAGCTAG
  AGTGACTGAGTTGAACCCAGACAAGAACTGCATTCACACAGATGACGACGAGAAGATCTCCTACCGATAT
  CTTATTATTGCTCTCGGAATCCAGCTGGACTATGAGAAGATTAAAGGCCTACCTGAAGGTTTCGCTCATC
  CCAAAATAGGGTCGAATTATTCAGTTAAGACTGTAGAGAAGACATGGAAAGCTCTGCAGGACTTCAAAGA
  GGGCAATGCCATCTTCACCTTCCCAAATACTCCAGTGAAGTGTGCTGGAGCCCCTCAGAAGATCATGTAC
  TTATCAGAAGCCTACTTCAGGAAGACAGGGAAGCGATCCAAGGCCAATATCATTTTCAACACTTCTCTTG
  GAGCCATTTTCGGGGTTAAGAAGTATGCAGATGCCCTGCAGGAGATCATCCAGGAGCGGAACCTCACTGT
  TAACTACAAGAAAAACCTCATTGAAGTCCGAGCCGATAAACAAGAGGCTGTATTTGAGAACCTGGACAAA
  CCAGGAGAGACCCAAGTGATTTCATATGAAATGCTTCATGTCACACCTCCAATGAGCCCACCAGATGTCC
  TCAAGACCAGTCCTGTGGCTGATGCTGCTGGTTGGGTGGATGTGGATAAAGAAACTCTGCAACACAGGAG
  GTACCCAAATGTGTTTGGGATTGGGGACTGCACCAACCTTCCTACGTCAAAGACCGCTGCTGCAGTAGCT
  GCCCAGTCAGGAATACTTGATAGGACAATTTCTGTAATTATGAAGAATCAAACACCAACAAAGAAGTATG
  ATGGCTACACATCATGTCCACTGGTGACCGGCTACAACCGTGTGATTCTTGCTGAGTTTGACTACAAAGC
  AGAGCCGCTAGAAACCTTCCCCTTTGATCAAAGCAAAGAGCGCCTTTCCATGTATCTCATGAAAGCTGAC
  CTGATGCCTTTCCTGTATTGGAATATGATGCTAAGGGGTTACTGGGGAGGACCAGCGTTTCTGCGCAAGT
  TGTTTCATCTAGGTATGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202335 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPLVAVVSGPRAQLFACLLRLGTQQVGPLQLHTGASHAARNHYEVLVLGGGSGGITMAARMKRKVGAEN
  VAIVEPSERHFYQPIWTLVGAGAKQLSSSGRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRY
  LIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMY
  LSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDK
  PGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVA
  AQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKAD
  LMPFLYWNMMLRGYWGGPAFLRKLFHLGMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021199
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021199.4
• RefSeq Size: 2003 bp
• RefSeq ORF: 1353 bp
• Locus ID: 58472
• UniProt ID: Q9Y6N5
• Cytogenetics: 15q21.1
• Protein Families: Druggable Genome
• MW: 50 kDa
• Gene Summary: The protein encoded by this gene may function in mitochondria to catalyze the conversion of sulfide to persulfides, thereby decreasing toxic concencrations of sulfide. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2012]