PSMD3 (NM_002809) Human Tagged ORF Clone

CAT#: RC202307

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PSMD3 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)



Reconstitution Protocol

USD 497.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PSMD3
Synonyms P58; RPN3; S3; TSTA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202307 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCAGGAGGGCTCGGCGCGGCGCCGCGGCGCGGACAAGGCGAAACCGCCGCCCGGCGGAGGAGAAC
AAGAACCCCCACCGCCGCCGGCCCCCCAGGATGTGGAGATGAAAGAGGAGGCAGCGACGGGTGGCGGGTC
AACGGGGGAGGCAGACGGCAAGACGGCGGCGGCAGCGGCTGAGCACTCCCAGCGAGAGCTGGACACAGTC
ACCTTGGAGGACATCAAGGAGCACGTGAAACAGCTAGAGAAAGCGGTTTCAGGCAAGGAGCCGAGATTCG
TGCTGCGGGCCCTGCGGATGCTGCCTTCCACATCACGCCGCCTCAACCACTATGTTCTGTATAAGGCTGT
GCAGGGCTTCTTCACTTCAAATAATGCCACTCGAGACTTTTTGCTCCCCTTCCTGGAAGAGCCCATGGAC
ACAGAGGCTGATTTACAGTTCCGTCCCCGCACGGGAAAAGCTGCGTCGAACACCCTCCTGCCTGAAGTGG
AAGCCTATCTCCAACTCCTCGTGGTCATCTTCATGATGAACAGCAAGCGCTACAAAGAGGCACAGAAGAT
CTCTGATGATCTGATGCAGAAGATCAGTACTCAGAACCGCCGGGCCCTAGACCTTGTAGCCGCAAAGTGT
TACTATTATCACGCCCGGGTCTATGAGTTCCTGGACAAGCTGGATGTGGTGCGCAGCTTCTTGCATGCTC
GGCTCCGGACAGCTACGCTTCGGCATGACGCAGACGGGCAGGCCACCCTGTTGAACCTCCTGCTGCGGAA
TTACCTACACTACAGCTTGTACGACCAGGCTGAGAAGCTGGTGTCCAAGTCTGTGTTCCCAGAGCAGGCC
AACAACAATGAGTGGGCCAGGTACCTCTACTACACAGGGCGAATCAAAGCCATCCAGCTGGAGTACTCAG
TGGCCCGGAGAACGATGACCAACGCCCTTCGCAAGGCCCCTCAGCACACAGCTGTCGGCTTCAAACAGAC
GGTGCACAAGCTTCTCATCGTGGTGGAGCTGTTGCTGGGGGAGATCCCTGACCGGCTGCAGTTCCGCCAG
CCCTCCCTCAAGCGCTCACTCATGCCCTATTTCCTTCTGACTCAAGCTGTCAGGACAGGAAACCTAGCCA
AGTTCAACCAGGTCCTGGATCAGTTTGGGGAGAAGTTTCAAGCAGATGGGACCTACACCCTAATTATCCG
GCTGCGGCACAACGTGATTAAGACAGGTGTACGCATGATCAGCCTCTCCTATTCCCGAATCTCCTTGGCT
GACATCGCCCAGAAGCTGCAGTTGGATAGCCCCGAAGATGCAGAGTTCATTGTTGCCAAGGCCATCCGGG
ATGGTGTCATTGAGGCCAGCATCAACCACGAGAAGGGCTATGTCCAATCCAAGGAGATGATTGACATCTA
TTCCACCCGAGAGCCCCAGCTAGCCTTCCACCAGCGCATCTCCTTCTGCCTAGATATCCACAACATGTCT
GTCAAGGCCATGAGGTTTCCTCCCAAATCGTACAACAAGGACTTGGAGTCTGCAGAGGAACGGCGTGAGC
GAGAACAGCAGGACTTGGAGTTTGCCAAGGAGATGGCAGAAGATGATGATGACAGCTTCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202307 protein sequence
Red=Cloning site Green=Tags(s)

MKQEGSARRRGADKAKPPPGGGEQEPPPPPAPQDVEMKEEAATGGGSTGEADGKTAAAAAEHSQRELDTV
TLEDIKEHVKQLEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVQGFFTSNNATRDFLLPFLEEPMD
TEADLQFRPRTGKAASNTLLPEVEAYLQLLVVIFMMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKC
YYYHARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQA
NNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQ
PSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLA
DIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMS
VKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002809
ORF Size 1602 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002809.4
RefSeq Size 2187 bp
RefSeq ORF 1605 bp
Locus ID 5709
UniProt ID O43242
Cytogenetics 17q21.1
Domains PCI
Protein Pathways Proteasome
MW 61 kDa
Gene Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]
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