MED30 (NM_080651) Human Tagged ORF Clone
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MED30 |
Synonyms | MED30S; THRAP6; TRAP25 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202305 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAGGCTCAGCAGG CCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACAGTGCAGGACATCGTGTACCG CACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTGCCAAATGGTGTCACTTACCACACTGGAACA TATCAAGACCGGTTAACAAAGCTACAGGATAATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGAT TGGTATATGACAAATGCAATGAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATA TGTGGAAGAAGATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATGGATCAATTAC GAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202305 protein sequence
Red=Cloning site Green=Tags(s) MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGT YQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERR EIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080651 |
ORF Size | 534 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080651.4 |
RefSeq Size | 994 bp |
RefSeq ORF | 537 bp |
Locus ID | 90390 |
UniProt ID | Q96HR3 |
Cytogenetics | 8q24.11 |
Protein Families | Druggable Genome, Transcription Factors |
MW | 20.3 kDa |
Gene Summary | The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202305L3 | Lenti ORF clone of Human mediator complex subunit 30 (MED30), Myc-DDK-tagged |
USD 600.00 |
|
RC202305L4 | Lenti ORF clone of Human mediator complex subunit 30 (MED30), mGFP tagged |
USD 600.00 |
|
RG202305 | MED30 (tGFP-tagged) - Human mediator complex subunit 30 (MED30) |
USD 500.00 |
|
SC322247 | MED30 (untagged)-Human mediator complex subunit 30 (MED30) |
USD 300.00 |