MED30 (NM_080651) Human Tagged ORF Clone

CAT#: RC202305

MED30 (Myc-DDK-tagged)-Human mediator complex subunit 30 (MED30)

Product images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MED30 (Cat# RC202305, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED30 (Cat# TA808041)(1:2000). Positive lysates LY409121 (100ug) and LC409121 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409121) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202305 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MED30 protein (Cat# TP302305). The protein was produced from HEK293T cells transfected with MED30 cDNA clone (Cat# RC202305) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MED30
• Synonyms: MED30S; THRAP6; TRAP25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202305 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCCCTCCGTTGGCCGCGTCGGGGATGGCGCCCGGGCCCTTCGCCGGGCCCCAGGCTCAGCAGG
  CCGCCCGGGAAGTCAACACGGCGTCGCTGTGCCGCATCGGGCAGGAGACAGTGCAGGACATCGTGTACCG
  CACCATGGAGATCTTCCAGCTCCTGAGGAACATGCAGCTGCCAAATGGTGTCACTTACCACACTGGAACA
  TATCAAGACCGGTTAACAAAGCTACAGGATAATCTTCGCCAACTTTCAGTTCTCTTCAGGAAGCTGAGAT
  TGGTATATGACAAATGCAATGAAAACTGTGGTGGGATGGATCCCATTCCAGTCGAGCAACTTATTCCATA
  TGTGGAAGAAGATGGCTCAAAGAATGATGATCGGGCTGGCCCACCTCGTTTTGCTAGTGAAGAGAGGCGA
  GAAATTGCTGAAGTAAATAAAAAACTCAAACAGAAGAATCAACAGCTGAAACAAATTATGGATCAATTAC
  GAAATCTCATCTGGGATATAAATGCCATGTTGGCAATGAGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202305 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQLPNGVTYHTGT
  YQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEEDGSKNDDRAGPPRFASEERR
  EIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080651
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080651.4
• RefSeq Size: 994 bp
• RefSeq ORF: 537 bp
• Locus ID: 90390
• UniProt ID: Q96HR3
• Cytogenetics: 8q24.11
• Protein Families: Druggable Genome, Transcription Factors
• MW: 20.3 kDa
• Gene Summary: The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]