G protein alpha 16 (GNA15) (NM_002068) Human Tagged ORF Clone

CAT#: RC202243

GNA15 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 15 (Gq class) (GNA15)

Product Images

Circular map for RC202243

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNA15 (Cat# RC202243, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNA15 (Cat# TA808136)(1:2000). Positive lysates LY419560 (100ug) and LC419560 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419560) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202243 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G protein alpha 16
• Synonyms: GNA16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202243 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCTCGCTGACCTGGCGCTGCTGCCCCTGGTGCCTGACGGAGGATGAGAAGGCCGCCGCCCGGG
  TGGACCAGGAGATCAACAGGATCCTCTTGGAGCAGAAGAAGCAGGACCGCGGGGAGCTGAAGCTGCTGCT
  TTTGGGCCCAGGCGAGAGCGGGAAGAGCACCTTCATCAAGCAGATGCGGATCATCCACGGCGCCGGCTAC
  TCGGAGGAGGAGCGCAAGGGCTTCCGGCCCCTGGTCTACCAGAACATCTTCGTGTCCATGCGGGCCATGA
  TCGAGGCCATGGAGCGGCTGCAGATTCCATTCAGCAGGCCCGAGAGCAAGCACCACGCTAGCCTGGTCAT
  GAGCCAGGACCCCTATAAAGTGACCACGTTTGAGAAGCGCTACGCTGCGGCCATGCAGTGGCTGTGGAGG
  GATGCCGGCATCCGGGCCTGCTATGAGCGTCGGCGGGAATTCCACCTGCTCGATTCAGCCGTGTACTACC
  TGTCCCACCTGGAGCGCATCACCGAGGAGGGCTACGTCCCCACAGCTCAGGACGTGCTCCGCAGCCGCAT
  GCCCACCACTGGCATCAACGAGTACTGCTTCTCCGTGCAGAAAACCAACCTGCGGATCGTGGACGTCGGG
  GGCCAGAAGTCAGAGCGTAAGAAATGGATCCATTGTTTCGAGAACGTGATCGCCCTCATCTACCTGGCCT
  CACTGAGTGAATACGACCAGTGCCTGGAGGAGAACAACCAGGAGAACCGCATGAAGGAGAGCCTCGCATT
  GTTTGGGACTATCCTGGAACTACCCTGGTTCAAAAGCACATCCGTCATCCTCTTTCTCAACAAAACCGAC
  ATCCTGGAGGAGAAAATCCCCACCTCCCACCTGGCTACCTATTTCCCCAGTTTCCAGGGCCCTAAGCAGG
  ATGCTGAGGCAGCCAAGAGGTTCATCCTGGACATGTACACGAGGATGTACACCGGGTGCGTGGACGGCCC
  CGAGGGCAGCAAGAAGGGCGCACGATCCCGACGCCTCTTCAGCCACTACACATGTGCCACAGACACACAG
  AACATCCGCAAGGTCTTCAAGGACGTGCGGGACTCGGTGCTCGCCCGCTACCTGGACGAGATCAACCTGC
  TG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202243 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGY
  SEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWR
  DAGIRACYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVG
  GQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTD
  ILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQ
  NIRKVFKDVRDSVLARYLDEINLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002068
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002068.4
• RefSeq Size: 2311 bp
• RefSeq ORF: 1125 bp
• Locus ID: 2769
• UniProt ID: P30679
• Cytogenetics: 19p13.3
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway
• MW: 43.5 kDa
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.[UniProtKB/Swiss-Prot Function]