G protein alpha 16 (GNA15) (NM_002068) Human Tagged ORF Clone

CAT#: RC202243

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GNA15 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha 15 (Gq class) (GNA15)



  "NM_002068" in other vectors (6)

Reconstitution Protocol

USD 457.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GNA15
Synonyms GNA16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202243 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGCTCGCTGACCTGGCGCTGCTGCCCCTGGTGCCTGACGGAGGATGAGAAGGCCGCCGCCCGGG
TGGACCAGGAGATCAACAGGATCCTCTTGGAGCAGAAGAAGCAGGACCGCGGGGAGCTGAAGCTGCTGCT
TTTGGGCCCAGGCGAGAGCGGGAAGAGCACCTTCATCAAGCAGATGCGGATCATCCACGGCGCCGGCTAC
TCGGAGGAGGAGCGCAAGGGCTTCCGGCCCCTGGTCTACCAGAACATCTTCGTGTCCATGCGGGCCATGA
TCGAGGCCATGGAGCGGCTGCAGATTCCATTCAGCAGGCCCGAGAGCAAGCACCACGCTAGCCTGGTCAT
GAGCCAGGACCCCTATAAAGTGACCACGTTTGAGAAGCGCTACGCTGCGGCCATGCAGTGGCTGTGGAGG
GATGCCGGCATCCGGGCCTGCTATGAGCGTCGGCGGGAATTCCACCTGCTCGATTCAGCCGTGTACTACC
TGTCCCACCTGGAGCGCATCACCGAGGAGGGCTACGTCCCCACAGCTCAGGACGTGCTCCGCAGCCGCAT
GCCCACCACTGGCATCAACGAGTACTGCTTCTCCGTGCAGAAAACCAACCTGCGGATCGTGGACGTCGGG
GGCCAGAAGTCAGAGCGTAAGAAATGGATCCATTGTTTCGAGAACGTGATCGCCCTCATCTACCTGGCCT
CACTGAGTGAATACGACCAGTGCCTGGAGGAGAACAACCAGGAGAACCGCATGAAGGAGAGCCTCGCATT
GTTTGGGACTATCCTGGAACTACCCTGGTTCAAAAGCACATCCGTCATCCTCTTTCTCAACAAAACCGAC
ATCCTGGAGGAGAAAATCCCCACCTCCCACCTGGCTACCTATTTCCCCAGTTTCCAGGGCCCTAAGCAGG
ATGCTGAGGCAGCCAAGAGGTTCATCCTGGACATGTACACGAGGATGTACACCGGGTGCGTGGACGGCCC
CGAGGGCAGCAAGAAGGGCGCACGATCCCGACGCCTCTTCAGCCACTACACATGTGCCACAGACACACAG
AACATCCGCAAGGTCTTCAAGGACGTGCGGGACTCGGTGCTCGCCCGCTACCTGGACGAGATCAACCTGC
TG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202243 protein sequence
Red=Cloning site Green=Tags(s)

MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGY
SEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWR
DAGIRACYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVG
GQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTD
ILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQ
NIRKVFKDVRDSVLARYLDEINLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002068
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002068.4
RefSeq Size 2311 bp
RefSeq ORF 1125 bp
Locus ID 2769
UniProt ID P30679
Cytogenetics 19p13.3
Domains G-alpha
Protein Families Druggable Genome
Protein Pathways Calcium signaling pathway
MW 43.5 kDa
Gene Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.[UniProtKB/Swiss-Prot Function]

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