DNAJA2 (NM_005880) Human Tagged ORF Clone

CAT#: RC202204

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DNAJA2 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2)



  "NM_005880" in other vectors (7)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DNAJA2
Synonyms CPR3; DJ3; DJA2; DNAJ; DNJ3; HIRIP4; PRO3015; RDJ2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202204 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAACGTGGCTGACACGAAGCTGTACGACATCCTGGGCGTCCCGCCCGGCGCCAGCGAGAACGAGC
TGAAGAAGGCATACAGAAAGTTAGCCAAGGAATATCATCCTGATAAGAATCCAAATGCAGGAGACAAATT
TAAAGAAATAAGTTTTGCATATGAAGTACTATCAAATCCTGAGAAGCGTGAGTTATATGACAGATACGGA
GAGCAAGGTCTTCGGGAAGGCAGCGGCGGAGGTGGTGGCATGGATGATATTTTCTCTCACATTTTTGGTG
GGGGATTGTTCGGCTTCATGGGCAATCAGAGTAGAAGTCGAAATGGCAGAAGAAGAGGAGAGGACATGAT
GCATCCACTCAAAGTATCTTTAGAAGATCTGTATAATGGCAAGACAACCAAACTACAACTTAGCAAGAAT
GTGCTCTGTAGTGCATGCAGTGGCCAAGGCGGAAAGTCTGGAGCTGTCCAAAAGTGTAGTGCTTGTCGAG
GTCGAGGTGTGCGCATCATGATCAGACAGCTGGCTCCAGGGATGGTACAACAGATGCAGTCTGTGTGCTC
TGATTGTAATGGAGAAGGAGAGGTAATTAATGAAAAAGACCGCTGTAAAAAATGTGAAGGGAAGAAGGTG
ATTAAAGAAGTCAAGATTCTTGAAGTCCACGTAGACAAAGGCATGAAACATGGACAGAGAATTACATTCA
CTGGGGAAGCAGACCAGGCCCCAGGAGTGGAACCCGGAGACATTGTTCTTTTGCTACAGGAGAAAGAACA
TGAGGTATTTCAGAGAGATGGGAATGATTTGCACATGACATATAAAATAGGACTTGTTGAAGCTCTATGT
GGATTTCAGTTCACATTTAAGCACCTTGATGGACGTCAGATTGTGGTGAAATACCCCCCTGGCAAAGTAA
TTGAACCAGGGTGTGTTCGTGTAGTTCGAGGTGAAGGGATGCCGCAGTATCGTAATCCCTTTGAAAAAGG
TGATCTTTACATAAAGTTTGATGTGCAGTTTCCTGAAAACAACTGGATCAACCCAGACAAGCTTTCTGAA
CTAGAAGATCTTCTGCCATCTAGACCGGAAGTTCCTAACATAATTGGAGAAACAGAGGAGGTAGAGCTTC
AGGAATTTGATAGCACTCGAGGCTCAGGAGGTGGTCAGAGGCGTGAAGCCTATAATGATAGCTCTGATGA
AGAAAGCAGCAGCCATCATGGACCTGGAGTGCAGTGTGCCCATCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202204 protein sequence
Red=Cloning site Green=Tags(s)

MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYG
EQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKN
VLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKV
IKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALC
GFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPENNWINPDKLSE
LEDLLPSRPEVPNIIGETEEVELQEFDSTRGSGGGQRREAYNDSSDEESSSHHGPGVQCAHQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005880
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005880.4
RefSeq Size 3066 bp
RefSeq ORF 1239 bp
Locus ID 10294
UniProt ID O60884
Cytogenetics 16q11.2
Domains DnaJ_CXXCXGXG, DnaJ, DnaJ_C
MW 45.7 kDa
Gene Summary The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro. [provided by RefSeq, Jul 2008]

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