FBXO8 (NM_012180) Human Tagged ORF Clone

CAT#: RC202154

FBXO8 (Myc-DDK-tagged)-Human F-box protein 8 (FBXO8)

Product Images

Circular map for RC202154

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FBXO8 (Cat# RC202154, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO8 (Cat# TA807551)(1:2000). Positive lysates LY415927 (100ug) and LC415927 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415927) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202154 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXO8
• Synonyms: DC10; FBS; FBX8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202154 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCAAGGGTTGTGGAGAGTGGTCAGAAACCAGCAGCTGCAACAAGAAGGCTACAGTGAGCAAGGCT
  ACCTCACCAGAGAGCAGAGCAGGAGAATGGCTGCGAGCAACATTTCTAACACCAATCATCGTAAACAAGT
  CCAAGGAGGCATTGACATATATCATCTTTTGAAGGCAAGGAAATCGAAAGAACAGGAAGGATTCATTAAT
  TTGGAAATGTTGCCTCCTGAGCTAAGCTTTACCATCTTGTCCTACCTGAATGCAACTGACCTTTGCTTGG
  CTTCATGTGTTTGGCAGGACCTTGCGAATGATGAACTTCTCTGGCAAGGGTTGTGCAAATCCACTTGGGG
  TCACTGTTCCATATACAATAAGAACCCACCTTTAGGATTTTCTTTTAGAAAATTGTATATGCAGCTGGAT
  GAAGGCAGCCTCACCTTTAATGCCAACCCAGATGAGGGAGTGAACTACTTTATGTCCAAGGGTATCCTGG
  ATGATTCGCCAAAGGAAATAGCAAAGTTTATCTTCTGTACAAGAACACTAAATTGGAAAAAACTGAGAAT
  CTATCTTGATGAAAGGAGAGATGTCTTGGATGACCTTGTAACATTGCATAATTTTAGAAATCAGTTCTTG
  CCAAATGCACTGAGAGAATTTTTTCGTCATATCCATGCCCCTGAAGAGCGTGGAGAGTATCTTGAAACTC
  TTATAACAAAGTTCTCACATAGATTCTGTGCTTGCAACCCTGATTTAATGCGAGAACTTGGCCTTAGTCC
  TGATGCTGTCTATGTACTGTGCTACTCTTTGATTCTACTTTCCATTGACCTCACTAGCCCTCATGTGAAG
  AATAAAATGTCAAAAAGGGAATTTATTCGAAATACCCGTCGCGCTGCTCAAAATATTAGTGAAGATTTTG
  TAGGGCATCTTTATGACAATATCTACCTTATTGGCCATGTGGCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202154 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQGLWRVVRNQQLQQEGYSEQGYLTREQSRRMAASNISNTNHRKQVQGGIDIYHLLKARKSKEQEGFIN
  LEMLPPELSFTILSYLNATDLCLASCVWQDLANDELLWQGLCKSTWGHCSIYNKNPPLGFSFRKLYMQLD
  EGSLTFNANPDEGVNYFMSKGILDDSPKEIAKFIFCTRTLNWKKLRIYLDERRDVLDDLVTLHNFRNQFL
  PNALREFFRHIHAPEERGEYLETLITKFSHRFCACNPDLMRELGLSPDAVYVLCYSLILLSIDLTSPHVK
  NKMSKREFIRNTRRAAQNISEDFVGHLYDNIYLIGHVAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012180
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012180.3
• RefSeq Size: 2564 bp
• RefSeq ORF: 960 bp
• Locus ID: 26269
• UniProt ID: Q9NRD0
• Cytogenetics: 4q34.1
• Domains: Sec7, F-box
• Protein Families: Druggable Genome
• MW: 37.1 kDa
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It contains a C-terminal amino acid sequence that bears a significant similarity with a portion of yeast Sec7p, a critical regulator of vesicular protein transport. This human protein may interact with ADP-ribosylation factor(s)(ARFs) and exhibit ARF-GEF (guanine nucleotide exchange factor) activity. [provided by RefSeq, Jul 2008]