GTF2IRD1 (NM_016328) Human Tagged ORF Clone

CAT#: RC202128

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GTF2IRD1 (Myc-DDK-tagged)-Human GTF2I repeat domain containing 1 (GTF2IRD1), transcript variant 1



  "NM_016328" in other vectors (7)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 851.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GTF2IRD1
Synonyms BEN; CREAM1; GTF3; hMusTRD1alpha1; MUSTRD1; RBAP2; WBS; WBSCR11; WBSCR12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202128 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTGCTGGGTAAGCGCTGTGACGTCCCCACCAACGGCTGCGGACCCGACCGCTGGAACTCCGCGT
TCACCCGCAAAGACGAGATCATCACCAGCCTCGTGTCTGCCTTAGACTCCATGTGCTCAGCGCTGTCCAA
ACTGAACGCCGAGGTGGCCTGTGTCGCCGTGCACGATGAGAGCGCCTTTGTGGTGGGCACAGAGAAGGGG
AGAATGTTCCTGAATGCCCGGAAGGAGCTACAGTCAGACTTCCTCAGGTTCTGCCGAGGGCCCCCGTGGA
AGGATCCGGAGGCAGAGCACCCCAAGAAGGTGCAGCGGGGCGAGGGTGGAGGCCGTAGCCTCCCTCGGTC
CTCCCTGGAACATGGCTCAGATGTGTACCTTCTGCGGAAGATGGTAGAGGAGGTGTTTGATGTTCTTTAT
AGCGAGGCCCTGGGAAGGGCCAGTGTGGTGCCACTGCCCTATGAGAGGCTGCTCAGGGAGCCAGGGCTGC
TGGCCGTGCAGGGGCTGCCCGAGGGCCTGGCCTTCCGAAGGCCAGCCGAGTATGACCCCAAGGCCCTCAT
GGCCATCCTGGAACACAGCCACCGCATCCGCTTCAAGCTCAAGAGGCCACTTGAGGATGGCGGGCGGGAC
TCGAAGGCCCTGGTGGAGCTGAACGGTGTCTCCCTGATTCCCAAGGGGTCACGGGACTGTGGCCTGCATG
GCCAGGCCCCCAAGGTGCCACCCCAGGACCTGCCCCCAACCGCCACCTCCTCCTCCATGGCCAGCTTCCT
GTACAGCACGGCGCTCCCCAACCACGCCATCCGAGAGCTCAAGCAGGAAGCACCTTCCTGCCCCCTTGCC
CCCAGCGACCTGGGCCTGAGTCGGCCCATGCCAGAGCCCAAGGCCACCGGTGCCCAAGACTTCTCCGACT
GTTGTGGACAGAAGCCCACTGGGCCTGGTGGGCCTCTCATCCAGAACGTCCATGCCTCCAAGCGCATTCT
CTTCTCCATCGTCCATGACAAGTCAGAGAAGTGGGACGCCTTCATAAAGGAAACCGAGGACATCAACACG
CTCCGGGAGTGTGTGCAGATCCTGTTTAACAGCAGATATGCGGAAGCCCTGGGCCTGGACCACATGGTCC
CCGTGCCCTACCGGAAGATTGCCTGTGACCCGGAGGCTGTGGAGATCGTGGGCATCCCGGACAAGATCCC
CTTCAAGCGCCCCTGCACTTACGGAGTCCCCAAGCTGAAGCGGATCCTGGAGGAGCGCCATAGTATCCAC
TTCATCATTAAGAGGATGTTTGATGAGCGAATTTTCACAGGGAACAAGTTTACCAAAGACACCACGAAGC
TGGAGCCAGCCAGCCCGCCAGAGGACACCTCTGCAGAGGTCTCTAGGGCCACCGTCCTTGACCTTGCTGG
GAATGCTCGGTCAGACAAGGGCAGCATGTCTGAAGACTGTGGGCCAGGAACCTCCGGGGAGCTGGGCGGG
CTGAGGCCGATCAAAATTGAGCCAGAGGATCTGGACATCATTCAGGTCACCGTCCCAGACCCCTCGCCAA
CCTCTGAGGAAATGACAGACTCGATGCCTGGGCACCTGCCATCGGAGGATTCTGGTTATGGGATGGAGAT
GCTGACAGACAAAGGTCTGAGTGAGGACGCGCGGCCCGAGGAGAGGCCCGTGGAGGACAGCCACGGTGAC
GTGATCCGGCCCCTGCGGAAGCAGGTGGAGCTGCTCTTCAACACACGATACGCCAAGGCCATTGGCATCT
CGGAGCCCGTCAAGGTGCCGTACTCCAAGTTTCTGATGCACCCGGAGGAGCTGTTTGTGGTGGGACTGCC
TGAAGGCATCTCCCTCCGCAGGCCCAACTGCTTCGGGATCGCCAAGCTCCGGAAGATTCTGGAGGCCAGC
AACAGCATCCAGTTTGTCATCAAGAGGCCCGAGCTGCTCACTGAGGGAGTCAAAGAGCCCATCGTGGATA
GTCAAGGAACTGCCTCCTCACTTGGCTTCTCTCCCCCTGCCCTGCCCCCAGAGAGGGATTCCGGGGACCC
TCTGGTGGACGAGAGCCTGAAGAGACAGGGCTTTCAAGAAAATTATGACGCGAGGCTCTCACGGATCGAC
ATCGCCAACACACTAAGGGAGCAGGTCCAGGACCTTTTCAATAAGAAATACGGGGAAGCCTTGGGCATCA
AGTACCCGGTCCAGGTCCCCTACAAGCGGATCAAGAGTAACCCCGGCTCCGTGATCATCGAGGGGCTGCC
CCCAGGAATCCCGTTCCGAAAGCCCTGTACCTTCGGCTCCCAGAACCTGGAGAGGATTCTTGCTGTGGCT
GACAAGATCAAGTTCACAGTCACCAGGCCTTTCCAAGGACTCATCCCAAAGCCTGATGAAGATGACGCCA
ACAGACTCGGGGAGAAGGTGATCCTGCGGGAGCAGGTGAAGGAACTCTTCAACGAGAAATACGGTGAGGC
CCTGGGCCTGAACCGGCCGGTGCTGGTCCCTTATAAACTAATCCGGGACAGCCCAGACGCCGTGGAGGTC
ACGGGTCTGCCTGATGACATCCCCTTCCGGAACCCCAACACGTACGACATCCACCGGCTGGAGAAGATCC
TGAAGGCCCGAGAGCATGTCCGCATGGTCATCATTAACCAGCTCCAACCCTTTGCAGAAATCTGCAATGA
TGCCAAGGTGCCAGCCAAAGACAGCAGCATTCCCAAGCGCAAGAGAAAGCGGGTCTCGGAAGGAAATTCC
GTCTCCTCTTCCTCCTCGTCTTCCTCTTCCTCGTCCTCTAACCCGGATTCAGTGGCATCGGCCAACCAGA
TCTCACTCGTGCAATGGCCAATGTACATGGTGGACTATGCCGGCCTGAACGTGCAGCTCCCGGGACCTCT
TAATTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202128 protein sequence
Red=Cloning site Green=Tags(s)

MALLGKRCDVPTNGCGPDRWNSAFTRKDEIITSLVSALDSMCSALSKLNAEVACVAVHDESAFVVGTEKG
RMFLNARKELQSDFLRFCRGPPWKDPEAEHPKKVQRGEGGGRSLPRSSLEHGSDVYLLRKMVEEVFDVLY
SEALGRASVVPLPYERLLREPGLLAVQGLPEGLAFRRPAEYDPKALMAILEHSHRIRFKLKRPLEDGGRD
SKALVELNGVSLIPKGSRDCGLHGQAPKVPPQDLPPTATSSSMASFLYSTALPNHAIRELKQEAPSCPLA
PSDLGLSRPMPEPKATGAQDFSDCCGQKPTGPGGPLIQNVHASKRILFSIVHDKSEKWDAFIKETEDINT
LRECVQILFNSRYAEALGLDHMVPVPYRKIACDPEAVEIVGIPDKIPFKRPCTYGVPKLKRILEERHSIH
FIIKRMFDERIFTGNKFTKDTTKLEPASPPEDTSAEVSRATVLDLAGNARSDKGSMSEDCGPGTSGELGG
LRPIKIEPEDLDIIQVTVPDPSPTSEEMTDSMPGHLPSEDSGYGMEMLTDKGLSEDARPEERPVEDSHGD
VIRPLRKQVELLFNTRYAKAIGISEPVKVPYSKFLMHPEELFVVGLPEGISLRRPNCFGIAKLRKILEAS
NSIQFVIKRPELLTEGVKEPIVDSQGTASSLGFSPPALPPERDSGDPLVDESLKRQGFQENYDARLSRID
IANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVA
DKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEV
TGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNS
VSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016328
ORF Size 2877 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016328.3
RefSeq Size 3471 bp
RefSeq ORF 2880 bp
Locus ID 9569
UniProt ID Q9UHL9
Cytogenetics 7q11.23
Domains GTF2I
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Basal transcription factors
MW 106 kDa
Gene Summary The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene plays a role in craniofacial and cognitive development and mutations have been associated with Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]

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