Selenium Binding Protein 1 (SELENBP1) (NM_003944) Human Tagged ORF Clone

CAT#: RC202108

SELENBP1 (Myc-DDK-tagged)-Human selenium binding protein 1 (SELENBP1)

Product Images

Circular map for RC202108

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SELENBP1 (Cat# RC202108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SELENBP1 (Cat# TA504700). Positive lysates LY401295 (100ug) and LC401295 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401295) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202108 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SELENBP1 protein (Cat# TP302108). The protein was produced from HEK293T cells transfected with SELENBP1 cDNA clone (Cat# RC202108) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Selenium Binding Protein 1
• Synonyms: EHMTO; HEL-S-134P; hSBP; LPSB; MTO; SBP56; SP56
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202108 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACGAAATGTGGGAATTGTGGACCCGGCTACTCCACCCCTCTGGAGGCCATGAAAGGACCCAGGG
  AAGAGATCGTCTACCTGCCCTGCATTTACCGAAACACAGGCACTGAGGCCCCAGATTATCTGGCCACTGT
  GGATGTTGACCCCAAGTCTCCCCAGTATTGCCAGGTCATCCACCGGCTGCCCATGCCCAACCTGAAGGAC
  GAGCTGCATCACTCAGGATGGAACACCTGCAGCAGCTGCTTCGGTGATAGCACCAAGTCGCGCACCAAGC
  TGGTGCTGCCCAGTCTCATCTCCTCTCGCATCTATGTGGTGGACGTGGGCTCTGAGCCCCGGGCCCCAAA
  GCTGCACAAGGTCATTGAGCCCAAGGACATCCATGCCAAGTGCGAACTGGCCTTTCTCCACACCAGCCAC
  TGCCTGGCCAGCGGGGAAGTGATGATCAGCTCCCTGGGAGACGTCAAGGGCAATGGCAAAGGGGGTTTTG
  TGCTGCTGGATGGGGAGACGTTCGAGGTGAAGGGGACATGGGAGAGACCTGGGGGTGCTGCACCGTTGGG
  CTATGACTTCTGGTACCAGCCTCGACACAATGTCATGATCAGCACTGAGTGGGCAGCTCCCAATGTCTTA
  CGAGATGGCTTCAACCCCGCTGATGTGGAGGCTGGACTGTACGGGAGCCACTTATATGTATGGGACTGGC
  AGCGCCATGAGATTGTGCAGACCCTGTCTCTAAAAGATGGGCTTATTCCCTTGGAGATCCGCTTCCTGCA
  CAACCCAGACGCTGCCCAAGGCTTTGTGGGCTGCGCACTCAGCTCCACCATCCAGCGCTTCTACAAGAAC
  GAGGGAGGTACATGGTCAGTGGAGAAGGTGATCCAGGTGCCCCCCAAGAAAGTGAAGGGCTGGCTGCTGC
  CCGAAATGCCAGGCCTGATCACCGACATCCTGCTCTCCCTGGACGACCGCTTCCTCTACTTCAGCAACTG
  GCTGCATGGGGACCTGAGGCAGTATGACATCTCTGACCCACAGAGACCCCGCCTCACAGGACAGCTCTTC
  CTCGGAGGCAGCATTGTTAAGGGAGGCCCTGTGCAAGTGCTGGAGGACGAGGAACTAAAGTCCCAGCCAG
  AGCCCCTAGTGGTCAAGGGAAAACGGGTGGCTGGAGGCCCTCAGATGATCCAGCTCAGCCTGGATGGGAA
  GCGCCTCTACATCACCACGTCGCTGTACAGTGCCTGGGACAAGCAGTTTTACCCTGATCTCATCAGGGAA
  GGCTCTGTGATGCTGCAGGTTGATGTAGACACAGTAAAAGGAGGGCTGAAGTTGAACCCCAACTTCCTGG
  TGGACTTCGGGAAGGAGCCCCTTGGCCCAGCCCTTGCCCATGAGCTCCGCTACCCTGGGGGCGATTGTAG
  CTCTGACATCTGGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202108 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATKCGNCGPGYSTPLEAMKGPREEIVYLPCIYRNTGTEAPDYLATVDVDPKSPQYCQVIHRLPMPNLKD
  ELHHSGWNTCSSCFGDSTKSRTKLVLPSLISSRIYVVDVGSEPRAPKLHKVIEPKDIHAKCELAFLHTSH
  CLASGEVMISSLGDVKGNGKGGFVLLDGETFEVKGTWERPGGAAPLGYDFWYQPRHNVMISTEWAAPNVL
  RDGFNPADVEAGLYGSHLYVWDWQRHEIVQTLSLKDGLIPLEIRFLHNPDAAQGFVGCALSSTIQRFYKN
  EGGTWSVEKVIQVPPKKVKGWLLPEMPGLITDILLSLDDRFLYFSNWLHGDLRQYDISDPQRPRLTGQLF
  LGGSIVKGGPVQVLEDEELKSQPEPLVVKGKRVAGGPQMIQLSLDGKRLYITTSLYSAWDKQFYPDLIRE
  GSVMLQVDVDTVKGGLKLNPNFLVDFGKEPLGPALAHELRYPGGDCSSDIWI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003944
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003944.4
• RefSeq Size: 1768 bp
• RefSeq ORF: 1419 bp
• Locus ID: 8991
• UniProt ID: Q13228
• Cytogenetics: 1q21.3
• MW: 52.4 kDa
• Gene Summary: This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]