RIP140 (NRIP1) (NM_003489) Human Tagged ORF Clone

CAT#: RC202101

NRIP1 (Myc-DDK-tagged)-Human nuclear receptor interacting protein 1 (NRIP1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418644) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202101 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NRIP1
• Synonyms: CAKUT3; RIP140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202101 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCATGGAGAAGAGCTTGGCTCTGATGTGCACCAGGATTCTATTGTTTTAACTTACCTAGAAGGAT
  TACTAATGCATCAGGCAGCAGGGGGATCAGGTACTGCCGTTGACAAAAAGTCTGCTGGGCATAATGAAGA
  GGATCAGAACTTTAACATTTCTGGCAGTGCATTTCCCACCTGTCAAAGTAATGGTCCAGTTCTCAATACA
  CATACATATCAGGGGTCTGGCATGCTGCACCTCAAAAAAGCCAGACTGTTGCAGTCTTCTGAGGACTGGA
  ATGCAGCAAAGCGGAAGAGGCTGTCTGATTCTATCATGAATTTAAACGTAAAGAAGGAAGCTTTGCTAGC
  TGGCATGGTTGACAGTGTGCCTAAAGGCAAACAGGATAGCACATTACTGGCCTCTTTGCTTCAGTCATTC
  AGCTCTAGGCTGCAGACTGTTGCTCTGTCACAACAAATCAGGCAGAGCCTCAAGGAGCAAGGATATGCCC
  TCAGTCATGATTCTTTAAAAGTGGAGAAGGATTTAAGGTGCTATGGTGTTGCATCAAGTCACTTAAAAAC
  TTTGTTGAAGAAAAGTAAAGTTAAAGATCAAAAGCCTGATACGAATCTTCCTGATGTGACTAAAAACCTC
  ATCAGAGATAGGTTTGCAGAGTCTCCTCATCATGTTGGACAAAGTGGAACAAAGGTCATGAGTGAACCGT
  TGTCATGTGCTGCAAGATTACAGGCTGTTGCAAGCATGGTGGAAAAAAGGGCTAGTCCTGCCACCTCACC
  TAAACCTAGTGTTGCTTGTAGCCAGTTAGCATTACTTCTGTCAAGCGAAGCCCATTTGCAGCAGTATTCT
  CGAGAACACGCTTTAAAAACGCAAAATGCAAATCAAGCAGCAAGTGAAAGACTTGCTGCTATGGCCAGAT
  TGCAAGAAAATGGCCAGAAGGATGTTGGCAGTTACCAGCTCCCAAAAGGAATGTCAAGCCATCTTAATGG
  TCAGGCAAGAACATCATCAAGCAAACTGATGGCTAGCAAAAGTAGTGCTACAGTGTTTCAAAATCCAATG
  GGTATCATTCCTTCTTCCCCTAAAAATGCAGGTTATAAGAACTCACTGGAAAGAAACAATATAAAACAAG
  CTGCTAACAATAGTTTGCTTTTACATCTTCTTAAAAGCCAGACTATACCTAAGCCAATGAATGGACACAG
  TCACAGTGAGAGAGGAAGCATTTTTGAGGAAAGTAGTACACCTACAACTATTGATGAATATTCAGATAAC
  AATCCTAGTTTTACAGATGACAGCAGTGGTGATGAAAGTTCTTATTCCAACTGTGTTCCCATAGACTTGT
  CTTGCAAACACCGAACTGAAAAATCAGAATCTGACCAACCTGTTTCCCTGGATAACTTCACTCAATCCTT
  GCTAAACACTTGGGATCCAAAAGTCCCAGATGTAGATATCAAAGAAGATCAAGATACCTCAAAGAATTCT
  AAGCTAAACTCACACCAGAAAGTAACACTTCTTCAATTGCTACTTGGCCATAAGAATGAAGAAAATGTAG
  AAAAAAACACCAGCCCTCAGGGAGTACACAATGATGTGAGCAAGTTCAATACACAAAATTATGCAAGGAC
  TTCTGTGATAGAAAGCCCCAGTACAAATCGGACTACTCCAGTGAGCACTCCACCTTTACTTACATCAAGC
  AAAGCAGGGTCTCCCATCAATCTCTCTCAACACTCTCTGGTCATCAAATGGAATTCCCCACCATATGTCT
  GCAGTACTCAGTCTGAAAAGCTAACAAATACTGCATCTAACCACTCAATGGACCTTACAAAAAGCAAAGA
  CCCACCAGGAGAGAAACCAGCCCAAAATGAAGGTGCACAGAACTCTGCAACGTTTAGTGCCAGTAAGCTG
  TTACAAAATTTAGCACAATGTGGAATGCAGTCATCCATGTCAGTGGAAGAGCAGAGACCCAGCAAACAGC
  TGTTAACTGGAAACACAGATAAACCGATAGGTATGATTGATAGATTAAATAGCCCTTTGCTCTCAAATAA
  AACAAATGCAGTTGAAGAAAATAAAGCATTTAGTAGTCAACCAACAGGTCCTGAACCAGGGCTTTCTGGT
  TCTGAAATAGAAAATCTGCTTGAAAGACGTACTGTCCTCCAGTTGCTCCTGGGGAACCCCAACAAAGGGA
  AGAGTGAAAAAAAAGAGAAAACTCCCTTAAGAGATGAAAGTACTCAGGAACACTCAGAGAGAGCTTTAAG
  TGAACAAATACTGATGGTGAAAATAAAATCTGAGCCTTGTGATGACTTACAAATTCCTAACACAAATGTG
  CACTTGAGCCATGATGCTAAGAGTGCCCCATTCTTGGGTATGGCTCCTGCTGTGCAGAGAAGCGCACCTG
  CCTTACCAGTGTCCGAAGACTTTAAATCGGAGCCTGTTTCACCTCAGGATTTTTCTTTCTCCAAGAATGG
  TCTGCTAAGTCGATTGCTAAGACAAAATCAAGATAGTTACCTGGCAGATGATTCAGACAGGAGTCACAGA
  AATAATGAAATGGCACTTCTAGAATCAAAGAATCTTTGCATGGTCCCTAAGAAAAGGAAGCTTTATACTG
  AGCCATTAGAAAATCCATTTAAAAAGATGAAAAACAACATTGTTGATGCTGCAAACAATCACAGTGCCCC
  AGAAGTACTGTATGGGTCCTTGCTTAACCAGGAAGAGCTGAAATTTAGCAGAAATGATCTTGAATTTAAA
  TATCCTGCTGGTCATGGCTCAGCCAGCGAAAGTGAACACAGGAGTTGGGCCAGAGAGAGCAAAAGCTTTA
  ATGTTCTGAAACAGCTGCTTCTCTCAGAAAACTGTGTGCGAGATTTGTCCCCGCACAGAATTAACTCTGT
  GGCTGACAGTAAAAAGAAAGGACACAAAAATAATGTGACCAACAGCAAACCTGAATTTAGCATTTCTTCT
  TTAAATGGACTGATGTACAGTTCCACTCAGCCCAGCAGTTGCATGGATAACAGGACATTTTCATACCCAG
  GTGTAGTAAAAACTCCTGTGAGTCCTACTTTCCCTGAGCACTTGGGCTGTGCAGGGTCTAGACCAGAATC
  TGGGCTTTTGAATGGGTGTTCCATGCCCAGTGAGAAAGGACCCATTAAGTGGGTTATCACTGATGCGGAG
  AAGAATGAGTATGAAAAAGACTCTCCAAGATTGACCAAAACCAACCCAATACTATATTACATGCTTCAAA
  AAGGAGGCAATTCTGTTACCAGTCGAGAAACACAAGACAAGGACATTTGGAGGGAGGCTTCATCTGCTGA
  AAGTGTCTCACAGGTCACAGCCAAAGAAGAGTTACTTCCTACTGCAGAAACGAAAGCTTCTTTCTTTAAT
  TTAAGAAGCCCTTACAATAGCCATATGGGAAATAATGCTTCTCGCCCACACAGCGCAAATGGAGAAGTTT
  ATGGACTTCTGGGAAGCGTGCTAACGATAAAGAAAGAATCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202101 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAVDKKSAGHNEEDQNFNISGSAFPTCQSNGPVLNT
  HTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIMNLNVKKEALLAGMVDSVPKGKQDSTLLASLLQSF
  SSRLQTVALSQQIRQSLKEQGYALSHDSLKVEKDLRCYGVASSHLKTLLKKSKVKDQKPDTNLPDVTKNL
  IRDRFAESPHHVGQSGTKVMSEPLSCAARLQAVASMVEKRASPATSPKPSVACSQLALLLSSEAHLQQYS
  REHALKTQNANQAASERLAAMARLQENGQKDVGSYQLPKGMSSHLNGQARTSSSKLMASKSSATVFQNPM
  GIIPSSPKNAGYKNSLERNNIKQAANNSLLLHLLKSQTIPKPMNGHSHSERGSIFEESSTPTTIDEYSDN
  NPSFTDDSSGDESSYSNCVPIDLSCKHRTEKSESDQPVSLDNFTQSLLNTWDPKVPDVDIKEDQDTSKNS
  KLNSHQKVTLLQLLLGHKNEENVEKNTSPQGVHNDVSKFNTQNYARTSVIESPSTNRTTPVSTPPLLTSS
  KAGSPINLSQHSLVIKWNSPPYVCSTQSEKLTNTASNHSMDLTKSKDPPGEKPAQNEGAQNSATFSASKL
  LQNLAQCGMQSSMSVEEQRPSKQLLTGNTDKPIGMIDRLNSPLLSNKTNAVEENKAFSSQPTGPEPGLSG
  SEIENLLERRTVLQLLLGNPNKGKSEKKEKTPLRDESTQEHSERALSEQILMVKIKSEPCDDLQIPNTNV
  HLSHDAKSAPFLGMAPAVQRSAPALPVSEDFKSEPVSPQDFSFSKNGLLSRLLRQNQDSYLADDSDRSHR
  NNEMALLESKNLCMVPKKRKLYTEPLENPFKKMKNNIVDAANNHSAPEVLYGSLLNQEELKFSRNDLEFK
  YPAGHGSASESEHRSWARESKSFNVLKQLLLSENCVRDLSPHRINSVADSKKKGHKNNVTNSKPEFSISS
  LNGLMYSSTQPSSCMDNRTFSYPGVVKTPVSPTFPEHLGCAGSRPESGLLNGCSMPSEKGPIKWVITDAE
  KNEYEKDSPRLTKTNPILYYMLQKGGNSVTSRETQDKDIWREASSAESVSQVTAKEELLPTAETKASFFN
  LRSPYNSHMGNNASRPHSANGEVYGLLGSVLTIKKESE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003489
• ORF Size: 3474 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003489.4
• RefSeq Size: 7556 bp
• RefSeq ORF: 3477 bp
• Locus ID: 8204
• UniProt ID: P48552
• Cytogenetics: 21q11.2-q21.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 127 kDa
• Gene Summary: Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008]