FA2H (NM_024306) Human Tagged ORF Clone

CAT#: RC202093

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FA2H (Myc-DDK-tagged)-Human fatty acid 2-hydroxylase (FA2H)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FA2H
Synonyms FAAH; FAH1; FAXDC1; SCS7; SPG35
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202093 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCGCTCCGCCCCCCGCCGCCTCCTTCTCGCCCTCCGAGGTCCAGCGGCGCCTGGCGGCCGGCG
CGTGCTGGGTCCGCCGCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGCGA
GCAGCTGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCGCACAGGCACTCG
GCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGCGGGGAGCAGCAGGGCTCCATGG
AGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACAGATCCTGCTATGGAACCACGGTTCAAAGTGGT
GGATTGGGACAAGGACCTGGTGGACTGGCGAAAGCCTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAG
TACGATGAGTGGGTTCACCAGCCGGTGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCC
TCTCTAAGACTGTCTGGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCCTA
CTACCGAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTACACGGTGGCAGTG
CCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGGAGCCTCATCGAGTACCTCATCC
ACCGCTTCCTGTTCCACATGAAGCCCCCCAGCGACAGCTATTACCTCATCATGCTGCACTTCGTCATGCA
CGGCCAGCACCACAAGGCACCCTTCGACGGCTCCCGCCTGGTCTTCCCCCCTGTGCCAGCCTCCCTGGTG
ATCGGCGTCTTCTACTTGTGCATGCAGCTCATCCTGCCCGAGGCAGTAGGGGGCACTGTGTTTGCGGGGG
GCCTCCTGGGCTACGTCCTCTATGACATGACCCATTACTACCTGCACTTTGGCTCGCCGCACAAGGGCTC
CTACCTGTACAGCCTGAAGGCCCACCACGTCAAGCACCACTTTGCACATCAGAAGTCAGGATTTGGTATC
AGCACTAAATTGTGGGATTACTGTTTCCACACCCTCACTCCAGAGAAACCCCACCTGAAGACGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202093 protein sequence
Red=Cloning site Green=Tags(s)

MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHS
ANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEK
YDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAV
PKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLV
IGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGI
STKLWDYCFHTLTPEKPHLKTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024306
ORF Size 1116 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024306.5
RefSeq Size 2450 bp
RefSeq ORF 1119 bp
Locus ID 79152
UniProt ID Q7L5A8
Cytogenetics 16q23.1
Protein Families Transmembrane
MW 42.8 kDa
Gene Summary This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]
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