BCL2A1 (NM_004049) Human Tagged ORF Clone
BCL2A1 (Myc-DDK-tagged)-Human BCL2-related protein A1 (BCL2A1), transcript variant 1
|Product Name||BCL2A1 (NM_004049) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ACC-1; ACC-2; ACC1; ACC2; BCL2L5; BFL1; GRS; HBPA1|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC201965 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201965 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||525 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_004049.1, NM_004049.2, NM_004049.3, NP_004040.1|
|RefSeq Size||899 bp|
|RefSeq ORF||528 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Metabolic pathways|
|Gene Summary||This gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Intrinsic Resistance to 5-Fluorouracil in a Brain Metastatic Variant of Human Breast Cancer Cell Line, MDA-MB-231BR
,Sagara, A;Igarashi, K;Otsuka, M;Karasawa, T;Gotoh, N;Narita, M;Kuzumaki, N;Narita, M;Kato, Y;,
PLoS ONE 2016
,PubMed ID 27723829
|cDNA Clone Resources|
|SC117596||BCL2A1 (untagged)-Human BCL2-related protein A1 (BCL2A1), transcript variant 1||
|RG201965||BCL2A1 (GFP-tagged) - Human BCL2-related protein A1 (BCL2A1), transcript variant 1||
|RC201965L1||Lenti ORF clone of Human BCL2-related protein A1 (BCL2A1), transcript variant 1, Myc-DDK-tagged||
|RC201965L2||Lenti ORF clone of Human BCL2-related protein A1 (BCL2A1), transcript variant 1, mGFP tagged||
|RC201965L3||Lenti ORF clone of Human BCL2-related protein A1 (BCL2A1), transcript variant 1, Myc-DDK-tagged||
|RC201965L4||Lenti ORF clone of Human BCL2-related protein A1 (BCL2A1), transcript variant 1, mGFP tagged||