ZNRD2 (NM_006396) Human Tagged ORF Clone

CAT#: RC201946

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SSSCA1 (Myc-DDK-tagged)-Human Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1)



  "NM_006396" in other vectors (6)

Reconstitution Protocol

USD 300.00

USD 450.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ZNRD2
Synonyms p27; SSSCA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201946 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGAACGGAGCTGAAGTCGACGACTTCTCCTGGGAGCCCCCGACTGAGGCGGAGACGAAGGTGC
TGCAGGCGCGACGGGAGCGGCAAGATCGCATCTCCCGGCTCATGGGCGACTATCTGCTGCGCGGTTACCG
CATGCTGGGCGAGACGTGTGCGGACTGCGGGACGATCCTCCTCCAAGACAAACAGCGGAAAATCTACTGC
GTGGCTTGTCAGGAACTCGACTCAGACGTGGATAAAGATAATCCCGCTCTGAATGCCCAGGCTGCCCTCT
CCCAAGCTCGGGAGCACCAGCTGGCCTCAGCCTCAGAGCTCCCCCTGGGCTCTCGACCTGCGCCCCAGCC
CCCAGTACCTCGTCCGGAGCACTGTGAGGGAGCTGCAGCAGGACTCAAGGCAGCCCAGGGGCCACCTGCT
CCTGCTGTGCCTCCAAATACAGATGTCATGGCCTGCACACAGACAGCCCTCTTGCAGAAGCTGACCTGGG
CCTCTGCTGAACTGGGCTCTAGCACCTCCCTGGAGACTAGCATCCAGCTGTGTGGCCTTATCCGCGCATG
TGCGGAGGCCCTGCGCAGCCTGCAGCAGCTACAGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201946 protein sequence
Red=Cloning site Green=Tags(s)

MALNGAEVDDFSWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYC
VACQELDSDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEGAAAGLKAAQGPPA
PAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETSIQLCGLIRACAEALRSLQQLQH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006396
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006396.3
RefSeq Size 661 bp
RefSeq ORF 600 bp
Locus ID 10534
UniProt ID O60232
Cytogenetics 11q13.1
MW 21.5 kDa
Gene Summary This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq, Jul 2008]

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