ZNRD2 (NM_006396) Human Tagged ORF Clone

CAT#: RC201946

SSSCA1 (Myc-DDK-tagged)-Human Sjogren syndrome/scleroderma autoantigen 1 (SSSCA1)

Product Images

Circular map for RC201946

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSSCA1 (Cat# RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1(Cat# TA501915). Positive lysates LY416665 (100ug) and LC416665 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY416665) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201946 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ZNRD2 protein (Cat# TP301946). The protein was produced from HEK293T cells transfected with ZNRD2 cDNA clone (Cat# RC201946) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNRD2
• Synonyms: p27; SSSCA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201946 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGAACGGAGCTGAAGTCGACGACTTCTCCTGGGAGCCCCCGACTGAGGCGGAGACGAAGGTGC
  TGCAGGCGCGACGGGAGCGGCAAGATCGCATCTCCCGGCTCATGGGCGACTATCTGCTGCGCGGTTACCG
  CATGCTGGGCGAGACGTGTGCGGACTGCGGGACGATCCTCCTCCAAGACAAACAGCGGAAAATCTACTGC
  GTGGCTTGTCAGGAACTCGACTCAGACGTGGATAAAGATAATCCCGCTCTGAATGCCCAGGCTGCCCTCT
  CCCAAGCTCGGGAGCACCAGCTGGCCTCAGCCTCAGAGCTCCCCCTGGGCTCTCGACCTGCGCCCCAGCC
  CCCAGTACCTCGTCCGGAGCACTGTGAGGGAGCTGCAGCAGGACTCAAGGCAGCCCAGGGGCCACCTGCT
  CCTGCTGTGCCTCCAAATACAGATGTCATGGCCTGCACACAGACAGCCCTCTTGCAGAAGCTGACCTGGG
  CCTCTGCTGAACTGGGCTCTAGCACCTCCCTGGAGACTAGCATCCAGCTGTGTGGCCTTATCCGCGCATG
  TGCGGAGGCCCTGCGCAGCCTGCAGCAGCTACAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201946 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALNGAEVDDFSWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGETCADCGTILLQDKQRKIYC
  VACQELDSDVDKDNPALNAQAALSQAREHQLASASELPLGSRPAPQPPVPRPEHCEGAAAGLKAAQGPPA
  PAVPPNTDVMACTQTALLQKLTWASAELGSSTSLETSIQLCGLIRACAEALRSLQQLQH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006396
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006396.3
• RefSeq Size: 661 bp
• RefSeq ORF: 600 bp
• Locus ID: 10534
• UniProt ID: O60232
• Cytogenetics: 11q13.1
• MW: 21.5 kDa
• Gene Summary: This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq, Jul 2008]