DNAJC12 (NM_021800) Human Tagged ORF Clone
DNAJC12 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201931 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201931 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||594 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1218 bp|
|RefSeq ORF||597 bp|
|Gene Summary||This gene encodes a member of a subclass of the HSP40/DnaJ protein family. Members of this family of proteins are associated with complex assembly, protein folding, and export. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC201931L3||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1, Myc-DDK-tagged||
|RC201931L4||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1, mGFP tagged||
|RG201931||DNAJC12 (GFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1||
|SC111830||DNAJC12 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1||
|SC322416||DNAJC12 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 12 (DNAJC12), transcript variant 1||