Ephrin B1 (EFNB1) (NM_004429) Human Tagged ORF Clone

CAT#: RC201886

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EFNB1 (Myc-DDK-tagged)-Human ephrin-B1 (EFNB1)



  "NM_004429" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol EFNB1
Synonyms CFND; CFNS; EFB1; EFL3; Elk-L; EPLG2; LERK2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201886 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCGGCCTGGGCAGCGTTGGCTCGGCAAGTGGCTTGTGGCGATGGTCGTGTGGGCGCTGTGCCGGC
TCGCCACACCGCTGGCCAAGAACCTGGAGCCCGTATCCTGGAGCTCCCTCAACCCCAAGTTCCTGAGTGG
GAAGGGCTTGGTGATCTATCCGAAAATTGGAGACAAGCTGGACATCATCTGCCCCCGAGCAGAAGCAGGG
CGGCCCTATGAGTACTACAAGCTGTACCTGGTGCGGCCTGAGCAGGCAGCTGCCTGTAGCACAGTTCTCG
ACCCCAACGTGTTGGTCACCTGCAATAGGCCAGAGCAGGAAATACGCTTTACCATCAAGTTCCAGGAGTT
CAGCCCCAACTACATGGGCCTGGAGTTCAAGAAGCACCATGATTACTACATTACCTCAACATCCAATGGA
AGCCTGGAGGGGCTGGAAAACCGGGAGGGCGGTGTGTGCCGCACACGCACCATGAAGATCATCATGAAGG
TTGGGCAAGATCCCAATGCTGTGACGCCTGAGCAGCTGACTACCAGCAGGCCCAGCAAGGAGGCAGACAA
CACTGTCAAGATGGCCACACAGGCCCCTGGTAGTCGGGGCTCCCTGGGTGACTCTGATGGCAAGCATGAG
ACTGTGAACCAGGAAGAGAAGAGTGGCCCAGGTGCAAGTGGGGGCAGCAGCGGGGACCCTGATGGCTTCT
TCAACTCCAAGGTGGCATTGTTCGCGGCTGTCGGTGCCGGTTGCGTCATCTTCCTGCTCATCATCATCTT
CCTGACGGTCCTACTACTGAAGCTACGCAAGCGGCACCGCAAGCACACACAGCAGCGGGCGGCTGCCCTC
TCGCTCAGTACCCTGGCCAGTCCCAAGGGGGGCAGTGGCACAGCGGGCACCGAGCCCAGCGACATCATCA
TTCCCTTACGGACTACAGAGAACAACTACTGCCCCCACTATGAGAAGGTGAGTGGGGACTACGGGCACCC
TGTCTACATCGTCCAAGAGATGCCGCCCCAGAGCCCGGCGAACATCTACTACAAGGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201886 protein sequence
Red=Cloning site Green=Tags(s)

MARPGQRWLGKWLVAMVVWALCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAG
RPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNRPEQEIRFTIKFQEFSPNYMGLEFKKHHDYYITSTSNG
SLEGLENREGGVCRTRTMKIIMKVGQDPNAVTPEQLTTSRPSKEADNTVKMATQAPGSRGSLGDSDGKHE
TVNQEEKSGPGASGGSSGDPDGFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAAL
SLSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004429
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004429.5
RefSeq Size 3344 bp
RefSeq ORF 1041 bp
Locus ID 1947
UniProt ID P98172
Cytogenetics Xq13.1
Domains Ephrin
Protein Families Druggable Genome, Transmembrane
Protein Pathways Axon guidance
MW 38 kDa
Gene Summary The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008]

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