SNX1 (NM_003099) Human Tagged ORF Clone

CAT#: RC201844

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SNX1 (Myc-DDK-tagged)-Human sorting nexin 1 (SNX1), transcript variant 1



  "NM_003099" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNX1
Synonyms HsT17379; VPS5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201844 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGCCTGGAGCCGG
AGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGACACCGAGGGGGAGGACATTTT
CACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAGATAACTACATCCCTTCTTCCCATCAACAAT
GGCTCCAAAGAAAATGGGATCCATGAAGAACAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAG
TGGAGCTATCCTTGGACAGCACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCC
TCCTCAGGAAGCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAATGCATATGTAG
CCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAGTTTGCAGTAAAAAGAAGATT
TAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAGCACTCTCAGAATGGCTTCATTGTCCCTCCA
CCCCCGGAGAAGAGCCTCATAGGGATGACAAAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAAT
TTCTTGAAAAACGGAGGGCCGCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACA
GGACCCTGACGTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATCAAGATGAATG
AATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAGCAGCGCTTACGGAAACTGCA
TGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTAGCGCTGAACACAGCCCAGTTTGCAAAGAGT
CTAGCCATGCTTGGGAGCTCTGAGGACAACACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGG
AAGAAAAAATTGAGCAGCTCCACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAG
TGACTACATTCGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCCAACAAGCCTG
ATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTGACTCAATATGAAAGGGACTT
CGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGGTTTGAGAAAGAGAAATCCAAGGACTTCAAG
AACCACGTGATCAAGTACCTTGAGACACTCCTTTACTCACAGCAGCAGCTGGCAAAGTACTGGGAAGCCT
TCCTTCCTGAGGCAAAGGCCATCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201844 protein sequence
Red=Cloning site Green=Tags(s)

MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPKITTSLLPINN
GSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQEATNSSKPQPTYEELEEEEQE
DQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPP
PPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLS
GAGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKS
LAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRW
QDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFK
NHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003099
ORF Size 1566 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003099.5
RefSeq Size 8332 bp
RefSeq ORF 1569 bp
Locus ID 6642
UniProt ID Q13596
Cytogenetics 15q22.31
Domains PX, Sorting_nexin
Protein Families Druggable Genome
MW 59.1 kDa
Gene Summary This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

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