MEK2 (MAP2K2) (NM_030662) Human Tagged ORF Clone
Lenti ORF clone of Human mitogen-activated protein kinase kinase 2 (MAP2K2), mGFP tagged
|Product Name||MEK2 (MAP2K2) (NM_030662) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CFC4; MAPKK2; MEK2; MKK2; PRKMK2|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1200 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1759 bp|
|RefSeq ORF||1203 bp|
|Domains||pkinase, TyrKc, S_TKc|
|Protein Families||Druggable Genome, Protein Kinase|
|Protein Pathways||Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chronic myeloid leukemia, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prion diseases, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway|
|Gene Summary||The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|SC110003||MAP2K2 (untagged)-Human mitogen-activated protein kinase kinase 2 (MAP2K2)||
|SC323627||MAP2K2 (untagged)-Kinase deficient mutant (K101M) of Human mitogen-activated protein kinase kinase 2 (MAP2K2)||
|RC201827||MAP2K2 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase 2 (MAP2K2)||
|RG201827||MAP2K2 (GFP-tagged) - Human mitogen-activated protein kinase kinase 2 (MAP2K2)||
|RC201827L1||Lenti ORF clone of Human mitogen-activated protein kinase kinase 2 (MAP2K2), Myc-DDK-tagged||
|RC201827L2||Lenti ORF clone of Human mitogen-activated protein kinase kinase 2 (MAP2K2), mGFP tagged||
|RC201827L3||Lenti ORF clone of Human mitogen-activated protein kinase kinase 2 (MAP2K2), Myc-DDK-tagged||