PEX16 (NM_057174) Human Tagged ORF Clone

CAT#: RC201813

PEX16 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 16 (PEX16), transcript variant 2

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_057174" in other vectors (5)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


PEX16 Antibody - middle region
    • 100 ul

USD 539.00

Other products for "PEX16"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PEX16
Synonyms PBD8A; PBD8B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201813 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGCTGCGGCTCCTGGGCCTCCGCTACCAGGAGTACGTGACTCGTCACCCGGCCGCCACGGCCC
AGCTGGAGACAGCAGTGCGGGGCTTCAGTTACCTGCTGGCAGGTCGATTCGCCGATTCGCACGAGCTGTC
AGAGCTGGTGTACTCTGCCTCTAACCTGCTTGTGCTGCTCAATGACGGGATCCTACGGAAGGAGCTTCGG
AAAAAGTTGCCTGTGTCGCTGTCCCAGCAGAAGCTGCTGACATGGCTGAGCGTGCTGGAGTGCGTGGAGG
TGTTCATGGAGATGGGAGCTGCCAAGGTGTGGGGTGAATTGGGCCGCTGGCTTGTCATCGCCCTCATCCA
GCTGGCCAAGGCTGTACTGCGGATTCTCCTGCTGCTCTGGTTCAAGGCTGGCCTCCAGACTTCACCCCCT
ATCGTTCCACTGGACAGAGAGACCCAGGCACAGCCCCCGGATGGTGACCACAGCCCTGGCAACCATGAGC
AGTCCTACGTGGGGAAGCGGTCAAACCGGGTGGTGCGAACCCTCCAGAACACGCCGTCCCTGCACTCCAG
GCACTGGGGAGCTCCCCAGCAGCGGGAGGGACGGCAGCAGCAGCATCACGAGGAGCTGAGTGCGACCCCC
ACCCCCCTGGGGCTGCAGGAGACCATCGCAGAGTTTTTGTACATTGCCCGGCCGCTGCTGCACTTGCTCA
GCCTGGGCCTGTGGGGTCAGAGGTCGTGGAAACCCTGGCTCTTGGCTGGTGTTGTGGACGTGACCAGCCT
GAGCCTCCTGAGTGACAGAAAGGGCCTGACCCGGAGGGAGCGGCGGGAGCTGCGGCGCCGGACCATCCTG
CTGCTCTACTACCTGCTGCGCTCTCCTTTCTACGACCGCTTCTCCGAGGCCAGGATCCTCTTCCTGCTCC
AGTTGCTGGCCGACCACGTCCCTGGCGTTGGCCTGGTCACAACGTCCCAGCGTGCGGCCTCTCCGTGCCT
ACCGGCCAGGCCCCACACACAGCCCTGGTCGCCACCAGCGTTCCTCCCAGGACACCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201813 protein sequence
Red=Cloning site Green=Tags(s)

MEKLRLLGLRYQEYVTRHPAATAQLETAVRGFSYLLAGRFADSHELSELVYSASNLLVLLNDGILRKELR
KKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGELGRWLVIALIQLAKAVLRILLLLWFKAGLQTSPP
IVPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTPSLHSRHWGAPQQREGRQQQHHEELSATP
TPLGLQETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLSDRKGLTRRERRELRRRTIL
LLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTTSQRAASPCLPARPHTQPWSPPAFLPGHP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_057174
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_057174.3
RefSeq Size 1794 bp
RefSeq ORF 1041 bp
Locus ID 9409
UniProt ID Q9Y5Y5
Cytogenetics 11p11.2
MW 39.3 kDa
Gene Summary The protein encoded by this gene is an integral peroxisomal membrane protein. An inactivating nonsense mutation localized to this gene was observed in a patient with Zellweger syndrome of the complementation group CGD/CG9. Expression of this gene product morphologically and biochemically restores the formation of new peroxisomes, suggesting a role in peroxisome organization and biogenesis. Alternative splicing has been observed for this gene and two variants have been described. [provided by RefSeq, Jul 2008]

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