PEX16 (NM_057174) Human Tagged ORF Clone

CAT#: RC201813

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PEX16 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 16 (PEX16), transcript variant 2



  "NM_057174" in other vectors (5)

Get anti-DDK tag antibody at $68 with code “DDK”. View details.

USD 433.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name PEX16 (NM_057174) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PEX16
Synonyms PBD8A; PBD8B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC201813 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGCTGCGGCTCCTGGGCCTCCGCTACCAGGAGTACGTGACTCGTCACCCGGCCGCCACGGCCC
AGCTGGAGACAGCAGTGCGGGGCTTCAGTTACCTGCTGGCAGGTCGATTCGCCGATTCGCACGAGCTGTC
AGAGCTGGTGTACTCTGCCTCTAACCTGCTTGTGCTGCTCAATGACGGGATCCTACGGAAGGAGCTTCGG
AAAAAGTTGCCTGTGTCGCTGTCCCAGCAGAAGCTGCTGACATGGCTGAGCGTGCTGGAGTGCGTGGAGG
TGTTCATGGAGATGGGAGCTGCCAAGGTGTGGGGTGAATTGGGCCGCTGGCTTGTCATCGCCCTCATCCA
GCTGGCCAAGGCTGTACTGCGGATTCTCCTGCTGCTCTGGTTCAAGGCTGGCCTCCAGACTTCACCCCCT
ATCGTTCCACTGGACAGAGAGACCCAGGCACAGCCCCCGGATGGTGACCACAGCCCTGGCAACCATGAGC
AGTCCTACGTGGGGAAGCGGTCAAACCGGGTGGTGCGAACCCTCCAGAACACGCCGTCCCTGCACTCCAG
GCACTGGGGAGCTCCCCAGCAGCGGGAGGGACGGCAGCAGCAGCATCACGAGGAGCTGAGTGCGACCCCC
ACCCCCCTGGGGCTGCAGGAGACCATCGCAGAGTTTTTGTACATTGCCCGGCCGCTGCTGCACTTGCTCA
GCCTGGGCCTGTGGGGTCAGAGGTCGTGGAAACCCTGGCTCTTGGCTGGTGTTGTGGACGTGACCAGCCT
GAGCCTCCTGAGTGACAGAAAGGGCCTGACCCGGAGGGAGCGGCGGGAGCTGCGGCGCCGGACCATCCTG
CTGCTCTACTACCTGCTGCGCTCTCCTTTCTACGACCGCTTCTCCGAGGCCAGGATCCTCTTCCTGCTCC
AGTTGCTGGCCGACCACGTCCCTGGCGTTGGCCTGGTCACAACGTCCCAGCGTGCGGCCTCTCCGTGCCT
ACCGGCCAGGCCCCACACACAGCCCTGGTCGCCACCAGCGTTCCTCCCAGGACACCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201813 protein sequence
Red=Cloning site Green=Tags(s)

MEKLRLLGLRYQEYVTRHPAATAQLETAVRGFSYLLAGRFADSHELSELVYSASNLLVLLNDGILRKELR
KKLPVSLSQQKLLTWLSVLECVEVFMEMGAAKVWGELGRWLVIALIQLAKAVLRILLLLWFKAGLQTSPP
IVPLDRETQAQPPDGDHSPGNHEQSYVGKRSNRVVRTLQNTPSLHSRHWGAPQQREGRQQQHHEELSATP
TPLGLQETIAEFLYIARPLLHLLSLGLWGQRSWKPWLLAGVVDVTSLSLLSDRKGLTRRERRELRRRTIL
LLYYLLRSPFYDRFSEARILFLLQLLADHVPGVGLVTTSQRAASPCLPARPHTQPWSPPAFLPGHP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_057174
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_057174.3
RefSeq Size 1794 bp
RefSeq ORF 1041 bp
Locus ID 9409
Cytogenetics 11p11.2
MW 39.3 kDa
Gene Summary The protein encoded by this gene is an integral peroxisomal membrane protein. An inactivating nonsense mutation localized to this gene was observed in a patient with Zellweger syndrome of the complementation group CGD/CG9. Expression of this gene product morphologically and biochemically restores the formation of new peroxisomes, suggesting a role in peroxisome organization and biogenesis. Alternative splicing has been observed for this gene and two variants have been described. [provided by RefSeq, Jul 2008]

Other Versions

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