MAP126 (SPAG5) (NM_006461) Human Tagged ORF Clone

CAT#: RC201783

SPAG5 (Myc-DDK-tagged)-Human sperm associated antigen 5 (SPAG5)

Product Images

Circular map for RC201783

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SPAG5 (Cat# RC201783, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPAG5 (Cat# TA810452)(1:2000). Positive lysates LY401941 (100ug) and LC401941 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401941) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201783 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SPAG5 protein (Cat# TP301783). The protein was produced from HEK293T cells transfected with SPAG5 cDNA clone (Cat# RC201783) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP126
• Synonyms: DEEPEST; hMAP126; MAP126
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201783 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGAGTGAAAAAACTGAGCCTCAGCCTGTCGCCTTCGCCCCAGACGGGAAAACCATCTATGAGAA
  CTCCTCTCCGTGAACTTACCCTGCAGCCCGGTGCCCTCACCAACTCTGGAAAAAGATCCCCCGCTTGCTC
  CTCGCTGACCCCATCACTGTGCAAGCTGGGGCTGCAGGAAGGCAGCAACAACTCATCTCCAGTGGATTTT
  GTAAATAACAAGAGGACAGACTTATCTTCAGAACATTTCAGTCATTCCTCAAAGTGGCTAGAAACTTGTC
  AGCATGAATCAGATGAGCAGCCTCTAGATCCAATTCCCCAAATTAGCTCTACTCCTAAAACGTCTGAGGA
  AGCAGTAGACCCACTGGGCAATTATATGGTTAAAACCATCGTCCTTGTACCATCTCCACTGGGGCAGCAA
  CAAGACATGATATTTGAGGCCCGTTTAGATACCATGGCAGAGACAAACAGCATATCTTTAAATGGACCTT
  TGAGAACAGACGATCTGGTGAGAGAGGAGGTGGCACCCTGCATGGGAGACAGGTTTTCAGAAGTTGCTGC
  TGTATCTGAGAAACCTATCTTTCAGGAATCTCCGTCCCATCTCTTAGAGGAGTCTCCACCAAATCCCTGT
  TCTGAACAACTACATTGCTCCAAGGAAAGCCTGAGCAGTAGAACTGAGGCTGTGCGTGAGGACTTAGTAC
  CTTCTGAAAGTAACGCCTTCTTGCCTTCCTCTGTTCTCTGGCTTTCCCCTTCAACTGCCTTGGCAGCAGA
  TTTCCGTGTCAATCATGTGGACCCAGAGGAGGAAATTGTAGAGCATGGAGCTATGGAGGAAAGAGAAATG
  AGGTTTCCCACACATCCTAAGGAGTCTGAAACAGAAGATCAAGCACTTGTCTCAAGTGTGGAAGATATTC
  TGTCCACATGCCTGACACCAAATCTAGTAGAAATGGAATCCCAAGAAGCTCCAGGCCCAGCAGTAGAAGA
  TGTTGGTAGGATTCTTGGCTCTGATACAGAGTCTTGGATGTCCCCACTGGCCTGGCTGGAAAAAGGTGTA
  AATACCTCCGTCATGCTGGAAAATCTCCGCCAAAGCTTATCCCTTCCCTCGATGCTTCGGGATGCTGCAA
  TTGGCACTACCCCTTTCTCTACTTGCTCGGTGGGGACTTGGTTTACTCCTTCAGCACCACAGGAAAAGAG
  TACAAACACATCCCAGACAGGCCTGGTTGGCACCAAGCACAGTACTTCTGAGACAGAGCAGCTCCTGTGT
  GGCCGGCCTCCAGATCTGACTGCCTTGTCTCGACATGACTTGGAAGATAACCTGCTGAGCTCTCTTGTCA
  TTCTGGAGGTTCTCTCCCGCCAGCTTCGGGACTGGAAGAGCCAGCTGGCTGTCCCTCACCCAGAAACCCA
  GGACAGTAGCACACAGACTGACACATCTCACAGTGGGATAACTAATAAACTTCAGCATCTTAAGGAGAGC
  CATGAGATGGGACAGGCCCTACAGCAGGCCAGAAATGTCATGCAATCATGGGTGCTTATCTCTAAAGAGC
  TGATATCCTTGCTTCACCTATCCCTGTTGCATTTAGAAGAAGATAAGACTACTGTGAGTCAGGAGTCTCG
  GCGTGCAGAAACATTGGTCTGTTGCTGTTTTGATTTGCTGAAGAAATTGAGGGCAAAGCTCCAGAGCCTC
  AAAGCAGAAAGGGAGGAGGCAAGGCACAGAGAGGAAATGGCTCTCAGAGGCAAGGATGCGGCAGAGATAG
  TGTTGGAGGCTTTCTGTGCACACGCCAGCCAGCGCATCAGCCAGCTGGAACAGGACCTAGCATCCATGCG
  GGAATTCAGAGGCCTTCTGAAGGATGCCCAGACCCAACTGGTAGGGCTTCATGCCAAGCAAGAAGAGCTG
  GTTCAGCAGACAGTGAGTCTTACTTCTACCTTGCAACAAGACTGGAGGTCCATGCAACTGGATTATACAA
  CATGGACAGCTTTGCTGAGTCGGTCCCGACAACTCACAGAGAAACTCACAGTCAAGAGCCAGCAAGCCCT
  GCAGGAACGTGATGTGGCAATTGAGGAAAAGCAGGAGGTTTCTAGGGTGCTGGAACAAGTCTCTGCCCAG
  TTAGAGGAGTGCAAAGGCCAAACAGAACAACTGGAGTTGGAAAACAGTCGTCTAGCAACAGATCTCCGGG
  CTCAGTTGCAGATTCTGGCCAACATGGACAGCCAGCTAAAAGAGCTACAGAGTCAGCATACCCATTGTGC
  CCAGGACCTGGCTATGAAGGATGAGTTACTCTGCCAGCTTACCCAGAGCAATGAGGAGCAGGCTGCTCAA
  TGGCAAAAGGAAGAGATGGCACTAAAACACATGCAGGCAGAACTGCAGCAGCAACAAGCTGTCCTGGCCA
  AAGAGGTGCGGGACCTGAAAGAGACCTTGGAGTTTGCAGACCAGGAGAATCAGGTTGCTCACCTGGAGCT
  GGGTCAGGTTGAGTGTCAATTGAAAACCACACTGGAAGTGCTCCGGGAGCGCAGCTTGCAGTGTGAGAAC
  CTCAAGGACACTGTAGAGAACCTAACGGCTAAACTGGCCAGCACCATAGCAGATAACCAGGAGCAAGATC
  TGGAGAAAACACGGCAGTACTCTCAAAAGCTAGGGCTGCTGACTGAGCAACTACAGAGCCTGACTCTCTT
  TCTACAGACAAAACTAAAGGAGAAGACTGAACAAGAGACCCTTCTGCTGAGTACAGCCTGTCCTCCCACC
  CAGGAACACCCTCTGCCTAATGACAGGACCTTCCTGGGAAGCATCTTGACAGCAGTGGCAGATGAAGAGC
  CAGAATCAACTCCTGTGCCCTTGCTTGGAAGTGACAAGAGTGCTTTCACCCGAGTAGCATCAATGGTTTC
  CCTTCAGCCCGCAGAGACCCCAGGCATGGAGGAGAGCCTGGCAGAAATGAGTATTATGACTACTGAGCTT
  CAGAGTCTTTGTTCCCTGCTACAAGAGTCTAAAGAAGAAGCCATCAGGACTCTGCAGCGAAAAATTTGTG
  AGCTGCAAGCTAGGCTGCAGGCCCAGGAAGAACAGCATCAGGAAGTCCAGAAGGCAAAAGAAGCAGACAT
  AGAGAAGCTGAACCAGGCCTTGTGCTTGCGCTACAAGAATGAAAAGGAGCTCCAGGAAGTGATACAGCAG
  CAGAATGAGAAGATCCTAGAACAGATAGACAAGAGTGGCGAGCTCATAAGCCTTAGAGAGGAGGTGACCC
  ACCTTACCCGCTCACTTCGGCGTGCGGAGACAGAGACCAAAGTGCTCCAGGAGGCCCTGGCAGGCCAGCT
  GGACTCCAACTGCCAGCCTATGGCCACCAATTGGATCCAGGAGAAAGTGTGGCTCTCTCAGGAGGTGGAC
  AAACTGAGAGTGATGTTCCTGGAGATGAAAAATGAGAAGGAAAAACTCATGATCAAGTTCCAGAGCCATA
  GAAATATCCTAGAGGAGAACCTTCGGCGCTCTGACAAGGAGTTAGAAAAACTAGATGACATTGTTCAGCA
  TATTTATAAGACCCTGCTCTCTATTCCAGAGGTGGTGAGGGGATGCAAAGAACTACAGGGATTGCTGGAA
  TTTCTGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201783 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWRVKKLSLSLSPSPQTGKPSMRTPLRELTLQPGALTNSGKRSPACSSLTPSLCKLGLQEGSNNSSPVDF
  VNNKRTDLSSEHFSHSSKWLETCQHESDEQPLDPIPQISSTPKTSEEAVDPLGNYMVKTIVLVPSPLGQQ
  QDMIFEARLDTMAETNSISLNGPLRTDDLVREEVAPCMGDRFSEVAAVSEKPIFQESPSHLLEESPPNPC
  SEQLHCSKESLSSRTEAVREDLVPSESNAFLPSSVLWLSPSTALAADFRVNHVDPEEEIVEHGAMEEREM
  RFPTHPKESETEDQALVSSVEDILSTCLTPNLVEMESQEAPGPAVEDVGRILGSDTESWMSPLAWLEKGV
  NTSVMLENLRQSLSLPSMLRDAAIGTTPFSTCSVGTWFTPSAPQEKSTNTSQTGLVGTKHSTSETEQLLC
  GRPPDLTALSRHDLEDNLLSSLVILEVLSRQLRDWKSQLAVPHPETQDSSTQTDTSHSGITNKLQHLKES
  HEMGQALQQARNVMQSWVLISKELISLLHLSLLHLEEDKTTVSQESRRAETLVCCCFDLLKKLRAKLQSL
  KAEREEARHREEMALRGKDAAEIVLEAFCAHASQRISQLEQDLASMREFRGLLKDAQTQLVGLHAKQEEL
  VQQTVSLTSTLQQDWRSMQLDYTTWTALLSRSRQLTEKLTVKSQQALQERDVAIEEKQEVSRVLEQVSAQ
  LEECKGQTEQLELENSRLATDLRAQLQILANMDSQLKELQSQHTHCAQDLAMKDELLCQLTQSNEEQAAQ
  WQKEEMALKHMQAELQQQQAVLAKEVRDLKETLEFADQENQVAHLELGQVECQLKTTLEVLRERSLQCEN
  LKDTVENLTAKLASTIADNQEQDLEKTRQYSQKLGLLTEQLQSLTLFLQTKLKEKTEQETLLLSTACPPT
  QEHPLPNDRTFLGSILTAVADEEPESTPVPLLGSDKSAFTRVASMVSLQPAETPGMEESLAEMSIMTTEL
  QSLCSLLQESKEEAIRTLQRKICELQARLQAQEEQHQEVQKAKEADIEKLNQALCLRYKNEKELQEVIQQ
  QNEKILEQIDKSGELISLREEVTHLTRSLRRAETETKVLQEALAGQLDSNCQPMATNWIQEKVWLSQEVD
  KLRVMFLEMKNEKEKLMIKFQSHRNILEENLRRSDKELEKLDDIVQHIYKTLLSIPEVVRGCKELQGLLE
  FLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006461
• ORF Size: 3579 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006461.4
• RefSeq Size: 3824 bp
• RefSeq ORF: 3582 bp
• Locus ID: 10615
• UniProt ID: Q96R06
• Cytogenetics: 17q11.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• MW: 134.4 kDa
• Gene Summary: This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. [provided by RefSeq, Jul 2008]