THUMPD1 (NM_017736) Human Tagged ORF Clone

CAT#: RC201773

THUMPD1 (Myc-DDK-tagged)-Human THUMP domain containing 1 (THUMPD1)

Product Images

Circular map for RC201773

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY THUMPD1 (Cat# RC201773, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-THUMPD1(Cat# TA505871). Positive lysates LY413587 (100ug) and LC413587 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413587) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201773 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THUMPD1
• Synonyms: Tan1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201773 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCCTGCCCAGCAGACTACTCAGCCTGGCGGCGGGAAGCGCAAAGGCAAGGCTCAGTATGTGC
  TGGCCAAGCGCGCTCGGCGCTGCGACGCTGGCGGGCCCCGTCAGCTAGAGCCCGGGCTACAGGGCATCCT
  CATCACCTGCAATATGAACGAGCGCAAGTGCGTGGAGGAGGCCTACAGCCTCCTCAACGAATACGGCGAC
  GACATGTATGGGCCAGAAAAGTTTACAGACAAGGATCAGCAGCCCTCTGGAAGTGAGGGAGAGGATGATG
  ATGCGGAGGCTGCCTTGAAGAAAGAAGTTGGTGACATTAAGGCATCTACAGAGATGAGGTTAAGAAGATT
  CCAGTCAGTGGAAAGTGGAGCAAATAACGTTGTCTTCATCAGGACACTTGGGATAGAGCCTGAGAAATTG
  GTGCATCATATTCTCCAGGATATGTACAAAACCAAGAAAAAGAAGACTCGAGTTATTTTGCGAATGTTAC
  CCATCTCAGGCACATGCAAGGCTTTTTTAGAAGATATGAAAAAATATGCAGAAACATTTTTGGAACCCTG
  GTTTAAAGCTCCAAACAAAGGGACATTTCAGATTGTGTACAAATCTCGAAATAACAGTCATGTGAATAGA
  GAAGAAGTTATCAGAGAATTGGCAGGAATAGTGTGCACCCTCAATTCAGAAAATAAAGTGGATCTCACCA
  ATCCACAGTACACAGTGGTAGTAGAAATCATCAAAGCTGTCTGTTGCCTGAGTGTTGTGAAAGATTACAT
  GTTGTTTAGAAAATACAATCTCCAGGAGGTGGTGAAGAGCCCTAAGGATCCGTCACAGCTTAACTCAAAG
  CAGGGAAATGGGAAAGAAGCTAAACTGGAATCTGCGGACAAATCAGACCAAAACAACACAGCAGAAGGAA
  AAAATAACCAGCAGGTACCAGAGAATACTGAGGAGCTGGGGCAGACAAAACCAACGTCTAATCCACAGGT
  GGTAAATGAGGGAGGAGCCAAACCTGAACTTGCAAGTCAAGCCACAGAAGGATCCAAGTCAAATGAAAAT
  GACTTCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201773 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPAQQTTQPGGGKRKGKAQYVLAKRARRCDAGGPRQLEPGLQGILITCNMNERKCVEEAYSLLNEYGD
  DMYGPEKFTDKDQQPSGSEGEDDDAEAALKKEVGDIKASTEMRLRRFQSVESGANNVVFIRTLGIEPEKL
  VHHILQDMYKTKKKKTRVILRMLPISGTCKAFLEDMKKYAETFLEPWFKAPNKGTFQIVYKSRNNSHVNR
  EEVIRELAGIVCTLNSENKVDLTNPQYTVVVEIIKAVCCLSVVKDYMLFRKYNLQEVVKSPKDPSQLNSK
  QGNGKEAKLESADKSDQNNTAEGKNNQQVPENTEELGQTKPTSNPQVVNEGGAKPELASQATEGSKSNEN
  DFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017736
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017736.5
• RefSeq Size: 4416 bp
• RefSeq ORF: 1062 bp
• Locus ID: 55623
• UniProt ID: Q9NXG2
• Cytogenetics: 16p12.3
• Domains: THUMP
• MW: 39.3 kDa
• Gene Summary: Functions as a tRNA-binding adapter to mediate NAT10-dependent tRNA acetylation (PubMed:25653167).[UniProtKB/Swiss-Prot Function]