HYPE (FICD) (NM_007076) Human Tagged ORF Clone

CAT#: RC201725

FICD (Myc-DDK-tagged)-Human FIC domain containing (FICD)

Product Images

Circular map for RC201725

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FICD (Cat# RC201725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD (Cat# TA802841). Positive lysates LY416213 (100ug) and LC416213 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416213) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201725 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FICD protein (Cat# TP301725). The protein was produced from HEK293T cells transfected with FICD cDNA clone (Cat# RC201725) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HYPE
• Synonyms: HIP13; HYPE; UNQ3041
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201725 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTCATACCAATGGCTTCAGTGATGGCGGTGACTGAACCGAAATGGGTCTCGGTCTGGAGCCGCT
  TCCTCTGGGTGACGCTGCTGAGCATGGTGCTGGGGTCCCTGCTGGCCCTGCTGCTGCCGCTGGGGGCTGT
  GGAGGAGCAGTGCTTGGCTGTGCTCAAAGGCCTCTACCTGCTCAGGAGCAAACCGGACAGGGCGCAGCAT
  GCCGCCACCAAGTGCACCAGCCCGTCCACGGAGCTCAGCATCACCTCCAGGGGCGCGACGCTGCTGGTGG
  CCAAGACCAAGGCCTCTCCAGCGGGTAAGTTGGAAGCCAGAGCTGCCCTGAACCAGGCCCTGGAGATGAA
  GCGCCAGGGCAAGCGGGAAAAAGCCCAAAAGCTCTTCATGCACGCCCTCAAGATGGACCCGGACTTCGTG
  GACGCGCTCACCGAGTTTGGCATCTTCTCGGAAGAAGACAAGGACATCATCCAGGCGGACTACTTGTACA
  CCAGAGCATTGACCATCTCACCCTACCATGAGAAAGCACTGGTCAACCGCGATCGGACACTGCCTCTTGT
  GGAAGAGATCGACCAGAGGTATTTCAGCATCATCGACAGCAAAGTGAAGAAGGTCATGTCCATCCCCAAG
  GGGAACTCAGCTCTGCGCAGGGTCATGGAGGAGACCTACTACCATCACATCTACCACACAGTGGCCATCG
  AGGGCAACACCCTCACCCTCTCGGAAATCAGGCACATCCTGGAGACCCGCTACGCCGTGCCCGGGAAGAG
  CCTGGAGGAGCAGAACGAGGTCATAGGCATGCATGCAGCCATGAAGTACATCAACACGACTCTGGTTTCG
  CGCATCGGCTCCGTCACCATCAGCGACGTGCTGGAGATCCACAGGCGGGTGCTGGGCTACGTGGACCCCG
  TGGAAGCCGGCAGGTTTCGGACAACACAGGTCCTGGTCGGACACCACATCCCTCCCCATCCGCAGGATGT
  GGAAAAGCAGATGCAGGAGTTTGTACAGTGGCTCAACTCCGAGGAAGCCATGAACCTGCACCCAGTGGAG
  TTTGCAGCCTTAGCCCATTATAAACTCGTTTACATCCACCCTTTCATTGATGGCAACGGGAGGACCTCCC
  GTCTGCTCATGAACCTCATCCTCATGCAGGCGGGCTACCCGCCCATCACCATCCGCAAGGAGCAGCGGTC
  CGACTACTACCACGTGTTGGAAGCTGCCAACGAGGGCGACGTGAGGCCTTTCATTCGCTTCATCGCCAAG
  TGTACTGAGACCACCCTGGACACCCTGCTTTTTGCCACAACTGAGTACTCGGTGGCACTGCCAGAAGCCC
  AACCCAACCACTCTGGGTTCAAGGAGACGCTTCCTGTGAAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201725 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMLIPMASVMAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQH
  AATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFV
  DALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPK
  GNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVS
  RIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVE
  FAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAK
  CTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007076
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007076.3
• RefSeq Size: 1651 bp
• RefSeq ORF: 1377 bp
• Locus ID: 11153
• UniProt ID: Q9BVA6
• Cytogenetics: 12q23.3
• Protein Families: Transmembrane
• MW: 51.8 kDa
• Gene Summary: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083).[UniProtKB/Swiss-Prot Function]