SNRP70 (SNRNP70) (NM_003089) Human Tagged ORF Clone

CAT#: RC201713

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SNRNP70 (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein 70kDa (U1) (SNRNP70)



  "NM_003089" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNRNP70
Synonyms RNPU1Z; RPU1; Snp1; SNRP70; U1-70K; U1AP; U1RNP; U170K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201713 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCAGTTCCTGCCGCCCAACCTTCTGGCCCTCTTTGCCCCCCGTGACCCTATTCCATACCTGCCAC
CCCTGGAGAAACTGCCACATGAAAAACACCACAATCAACCTTATTGTGGCATTGCGCCGTACATTCGAGA
GTTTGAGGACCCTCGAGATGCCCCTCCTCCAACTCGTGCTGAAACCCGAGAGGAGCGCATGGAGAGGAAA
AGACGGGAAAAGATTGAGCGGCGACAGCAAGAAGTGGAGACAGAGCTTAAAATGTGGGACCCTCACAATG
ATCCCAATGCTCAGGGGGATGCCTTCAAGACTCTCTTCGTGGCGAGAGTGAATTATGACACAACAGAATC
CAAGCTCCGGAGAGAGTTTGAGGTGTACGGACCTATCAAAAGAATACACATGGTCTACAGTAAGCGGTCA
GGAAAGCCCCGTGGCTATGCCTTCATCGAGTACGAACACGAGCGAGACATGCACTCCGCTTACAAACACG
CAGATGGCAAGAAGATTGATGGCAGGAGGGTCCTTGTGGACGTGGAGAGGGGCCGAACCGTGAAGGGCTG
GAGGCCCCGGCGGCTAGGAGGAGGCCTCGGTGGTACCAGAAGAGGAGGGGCTGATGTGAACATCCGGCAT
TCAGGCCGCGATGACACCTCCCGCTACGATGAGAGGCCCGGCCCCTCCCCGCTTCCGCACAGGGACCGGG
ACCGGGACCGTGAGCGGGAGCGCAGAGAGCGGAGCCGGGAGCGAGACAAGGAGCGAGAACGGCGACGCTC
CCGCTCCCGGGACCGGCGGAGGCGCTCACGGAGTCGCGACAAGGAGGAGCGGAGGCGCTCCAGGGAGCGG
AGCAAGGACAAGGACCGGGACCGGAAGCGGCGAAGCAGCCGGAGTCGGGAGCGGGCCCGGCGGGAGCGGG
AGCGCAAGGAGGAGCTGCGTGGTGGCGGTGGCGACATGGCGGAGCCCTCCGAGGCGGGTGACGCGCCCCC
TGATGATGGGCCTCCAGGGGAGCTCGGGCCTGACGGCCCTGACGGTCCAGAGGAAAAGGGCCGGGATCGT
GACCGGGAGCGACGGCGGAGCCACCGGAGCGAGCGCGAGCGGCGCCGGGACCGGGATCGTGACCGTGACC
GTGACCGCGAGCACAAACGGGGGGAGCGGGGCAGTGAGCGGGGCAGGGATGAGGCCCGAGGTGGGGGCGG
TGGCCAGGACAACGGGCTGGAGGGTCTGGGCAACGACAGCCGAGACATGTACATGGAGTCTGAGGGCGGC
GACGGCTACCTGGCTCCGGAGAATGGGTATTTGATGGAGGCTGCGCCGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201713 protein sequence
Red=Cloning site Green=Tags(s)

MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERK
RREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRS
GKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRH
SGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRER
SKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDR
DRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGG
DGYLAPENGYLMEAAPE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003089
ORF Size 1311 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003089.3
RefSeq Size 2008 bp
RefSeq ORF 1314 bp
Locus ID 6625
UniProt ID P08621
Cytogenetics 19q13.33
Protein Families Druggable Genome
Protein Pathways Spliceosome
MW 51.6 kDa
Gene Summary Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome (PubMed:19325628, PubMed:25555158). SNRNP70 binds to the loop I region of U1-snRNA (PubMed:2467746, PubMed:19325628, PubMed:25555158).[UniProtKB/Swiss-Prot Function]

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