NUCKS1 (NM_022731) Human Tagged ORF Clone

CAT#: RC201704

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NUCKS1 (Myc-DDK-tagged)-Human nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1)



  "NM_022731" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


Availability*
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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NUCKS1
Synonyms JC7; NUCKS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201704 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGGCCTGTCAGAAATAGGAAGGTTGTTGATTACTCACAGTTTCAGGAATCTGATGATGCAGATG
AAGATTATGGAAGAGATTCGGGCCCTCCCACTAAGAAAATTCGATCATCTCCCCGAGAAGCTAAAAATAA
GAGGCGATCTGGAAAGAATTCACAGGAAGATAGTGAGGACTCAGAAGACAAAGATGTGAAGACCAAGAAG
GATGATTCTCACTCAGCAGAGGATAGTGAAGATGAAAAAGAAGATCATAAAAATGTGCGCCAACAACGGC
AGGCGGCATCTAAAGCAGCTTCTAAACAGAGAGAGATGCTCATGGAAGATGTGGGCAGTGAGGAAGAACA
AGAAGAGGAGGATGAGGCACCATTCCAGGAGAAAGATTCCGGCAGCGATGAAGATTTCCTAATGGAAGAT
GATGACGATAGTGACTATGGCAGTTCGAAAAAGAAAAACAAAAAGATGGTTAAGAAGTCCAAACCTGAAA
GAAAAGAAAAGAAAATGCCCAAACCCAGACTAAAGGCTACAGTGACGCCAAGTCCAGTGAAAGGCAAAGG
GAAAGTGGGTCGCCCCACAGCTTCAAAGGCATCAAAGGAAAAGACTCCTTCTCCCAAAGAAGAAGATGAG
GAACCGGAAAGCCCGCCAGAAAAGAAAACATCTACAAGCCCCCCACCCGAGAAATCTGGGGATGAAGGGT
CTGAAGATGAAGCCCCTTCTGGGGAGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201704 protein sequence
Red=Cloning site Green=Tags(s)

MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKK
DDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMED
DDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDE
EPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022731
ORF Size 729 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022731.5
RefSeq Size 6471 bp
RefSeq ORF 732 bp
Locus ID 64710
UniProt ID Q9H1E3
Cytogenetics 1q32.1
Protein Families Druggable Genome
MW 27.3 kDa
Gene Summary This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. [provided by RefSeq, Aug 2010]

Other Versions

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