NUCKS1 (NM_022731) Human Tagged ORF Clone

CAT#: RC201704

NUCKS1 (Myc-DDK-tagged)-Human nuclear casein kinase and cyclin-dependent kinase substrate 1 (NUCKS1)

Product Images

Circular map for RC201704

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  Western blot validation of overexpression lysate (Cat# LY411597) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201704 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NUCKS1 protein (Cat# TP301704). The protein was produced from HEK293T cells transfected with NUCKS1 cDNA clone (Cat# RC201704) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NUCKS1
• Synonyms: JC7; NUCKS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201704 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGCCTGTCAGAAATAGGAAGGTTGTTGATTACTCACAGTTTCAGGAATCTGATGATGCAGATG
  AAGATTATGGAAGAGATTCGGGCCCTCCCACTAAGAAAATTCGATCATCTCCCCGAGAAGCTAAAAATAA
  GAGGCGATCTGGAAAGAATTCACAGGAAGATAGTGAGGACTCAGAAGACAAAGATGTGAAGACCAAGAAG
  GATGATTCTCACTCAGCAGAGGATAGTGAAGATGAAAAAGAAGATCATAAAAATGTGCGCCAACAACGGC
  AGGCGGCATCTAAAGCAGCTTCTAAACAGAGAGAGATGCTCATGGAAGATGTGGGCAGTGAGGAAGAACA
  AGAAGAGGAGGATGAGGCACCATTCCAGGAGAAAGATTCCGGCAGCGATGAAGATTTCCTAATGGAAGAT
  GATGACGATAGTGACTATGGCAGTTCGAAAAAGAAAAACAAAAAGATGGTTAAGAAGTCCAAACCTGAAA
  GAAAAGAAAAGAAAATGCCCAAACCCAGACTAAAGGCTACAGTGACGCCAAGTCCAGTGAAAGGCAAAGG
  GAAAGTGGGTCGCCCCACAGCTTCAAAGGCATCAAAGGAAAAGACTCCTTCTCCCAAAGAAGAAGATGAG
  GAACCGGAAAGCCCGCCAGAAAAGAAAACATCTACAAGCCCCCCACCCGAGAAATCTGGGGATGAAGGGT
  CTGAAGATGAAGCCCCTTCTGGGGAGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201704 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPTKKIRSSPREAKNKRRSGKNSQEDSEDSEDKDVKTKK
  DDSHSAEDSEDEKEDHKNVRQQRQAASKAASKQREMLMEDVGSEEEQEEEDEAPFQEKDSGSDEDFLMED
  DDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKGKVGRPTASKASKEKTPSPKEEDE
  EPESPPEKKTSTSPPPEKSGDEGSEDEAPSGED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022731
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022731.5
• RefSeq Size: 6471 bp
• RefSeq ORF: 732 bp
• Locus ID: 64710
• UniProt ID: Q9H1E3
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• MW: 27.3 kDa
• Gene Summary: This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. [provided by RefSeq, Aug 2010]